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Articles by L. Hassan
Total Records ( 6 ) for L. Hassan
  Soe Soe Wai , A.A. Saleha , Z. Zunita , L. Hassan , A. Jalila and A.H. Shah
  Helicobacter pullorum, an enterohepatic helicobacter with the ability to colonize the intestine and liver of avian species, mice and humans, is increasingly recognized as a food borne zoonotic pathogen. The present study aimed to determine the genetic similarity among H. pullorum using pulsed field gel electrophoresis (PFGE) with SacII and SmaI restriction endonucleases (REs) digestion. The H. pullorum were isolated from broiler chickens in eight farms. Within-farm genetic profiling of isolates showed close relationship (>95%); however genetic diversity was observed between isolates from different farms. Digestion of genome with SacII yielded two clusters comprised 16 pulsotypes, 8-14 DNA bands with a molecular weight ranging from 40 to 400 kb whereas SmaI digested genome produced two clusters with 14 pulsotypes, 7 to 13 DNA bands with molecular weight ranging from 40 to 250 kb. The RE SacII showed a higher discriminatory power compared to SmaI. In conclusion, within-farm genetic similarity of isolates suggested the same source of clonal origin.
  I.M. Ahmed , S. Khairani-Bejo , L. Hassan , A.R. Bahaman and A.R. Omar
  The potential diagnostic ability of Recombinant Outer Membrane Proteins (rOMPs), a combination of equal concentrations of rOMP25, rOMP28 and rOMP31of Brucella melitensis was investigated using Enzyme-Linked Immunosorbent Assay (ELISA) to differentiate the False Positive Serological Reactions (FPSR) in the serological diagnosis of caprine brucellosis. The rOMPs was tested using sera from three groups of goats with known Brucella exposure status which represent, naturally B. melitensis infected goats (infected), Brucella free goats (non-infected) and goats vaccinated with B. melitensis Rev. 1 vaccine strain (vaccinated). Additionally, all the sera were tested using the common serological tests which are Rose Bengal Plate Test (RBPT), BRUCELISA-400SG and Complement Fixation Test (CFT). When testing infected and non infected groups, the rOMPs I-ELISA recorded 94.44% (34/36) sensitivity and 100% (36/36) specificity and this almost agreed with the results obtained from testing the same serum samples using RBPT, BRUCELISA-400SG and CFT. However, when goats vaccinated with B. melitensis Rev. 1 vaccine strain were tested by the common serological tests, RBPT, BRUCELISA-400SG and CFT they wrongly recorded positive results for all the tested serum samples (26/26). While the developed rOMPs I-ELISA was able to differentiate the vaccinated from infected animals with 94.44 sensitivity and 84.62% specificity. The potential diagnostic ability of rOMPs would be of great importance as serologic marker to minimize the FPSR in eradication programs of caprine brucellosis.
  P.H. Bamaiyi , L. Hassan , S. Khairani-Bejo , A. Adzhar and M. Ramlan
  A study was conducted to detect Brucellosis in pigs using the Brucella abortus Complement Fixation Test (CFT). Out of a total sample of 552 pigs 3 (0.54%) were positive for Brucellosis. This result has epidemiological implications for the transmission of brucellosis in Malaysia and beyond due to the interaction of pigs with other animals and the human population.
  L. Hassan and S.D. Ahmad
  Colchicine at various concentration was given to the apical buds of maize (Zea mays L.) Plants, in order to induce tetraploidy for meiotic studies. Highly irregular meiosis in the C1 generation was revealed. These meiotic abnormalities increased pollen sterility which lead to a lower seed setting. In the C2 generation the occurrence of bivalents has increased and that of multivalents has decreased. Restoration of meiotic regularities, pollen fertility.
  L. Hassan and S.D. Ahmad
  The effect of chemical mutagens on the induction of mitotic chromosomal aberrations in diploid Allium cepa and tetraploid Allium vineale has been studied. In inducing chromosomal aberrations in case of both diploid and tetraploid Allium all the mutagens were found to be effective . Mitotic irregularities were higher in tetraploid species as compared with the diploid species. All types of chemical mutagens were found to be effective for the induction of chromosomal aberrations but chromosomal aberrations are increased due to the effect of Ethyl Methyl Sulphonate (EMS) in comparison to the other chemical mutagens. In explaining the concentration of EMS application of 0.20 % of the chemical may be the highest dose whereas 0.05 % may be considered as a lower dose.
  L. Hassan , S.D. Ahmad , A. Okumus and M. Wazuddin
  A 3 hr. period treatment with a 0.2% aqueous solution of colchicine was given to one week old seedlings of seven inbred lines of wheat (Triticum aestivum L) plants. Comparisons were made in the CT1 generation between the untreated controls (2x) and colchicine treated plants (C2x) for variation of leaf mesophyll cell size and chloroplast number. Differences were found in that the cell section area and the number of chloroplasts per cell in all the seven lines investigated were significantly greater in the C2x selection compared with the 2x controls using coded determination. Heritable differences were found such that the cell selection areas and the numbers of chloroplasts per cell were significantly greater in the C2x treatment in all the lines. In each case there was an enhancement of chloroplast number independent of the variation in cell size.
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