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Articles by Krishna Dutta Rawat
Total Records ( 2 ) for Krishna Dutta Rawat
  Kundan Kumar Chaubey , Shoor Vir Singh , Saurabh Gupta , Sujata Jayaraman , Manju Singh , Bjorn John Stephan , Krishna Dutta Rawat , Anjali Pachoori , Kuldeep Dhama and Ashok Kumar Bhatia
  Cross reactivity of three antigens of Mycobacterium avium subspecies paratuberculosis with sera of sheep endemic for Johne’s disease was evaluated. Out of 40 sheep tested by fecal microscopy, 72.5% were shedding MAP. Using protoplasmic antigens (PPA) from three MAP strains isolated from different livestock species and geographical regions, 90, 77.5 and 2.5% sheep were positive in goat (Indigenous g-ELISA) and cattle (b-ELISA) based ELISA kits and ELISA kit for small ruminant (sr-ELISA), respectively. Only 2.5 and 10% sheep were positive and negative in all the four tests. Native species specific (goat origin novel ‘Indian Bison Type’ MAP) semi-purified whole cell PPA based ELISA (Indigenous g-ELISA) was superior in reacting with sera of native sheep than the commercial PPA of bovine origin (Allied Monitor Inc., USA) and commercial ELISA kit for small ruminants (ID Vet, France). Lower cross reactivity of antigens originated from US and France emphasized the need to develop tests based on local strain of MAP than strains from different livestock species and geographical regions. This is an important finding against the use of ‘Global kits’ without validating in local conditions. Study showed that kits developed from local strains of MAP were not only superior but also cost effective and will significantly contribute in programs for the control of JD in native sheep population.
  Shoor Vir Singh , Saurabh Gupta , Kundan Kumar Chaubey , Krishna Dutta Rawat , Naveen Kumar , Jagdip Singh Sohal , Sarjeet Singh , Ruchi Tiwari , Sandip Chakraborty and Kuldeep Dhama
  Bovine Johne’s Disease (BJD) is a chronic granulomatous enteritis that affects ruminants worldwide and is having significant impact on the world economy and has been frequently reported from farm and farmer’s herds. An attack of Johne’s disease in a newly established cattle dairy farm consisting of high yielding Holstein Friesian (HF) cows in the Alwar district of Rajasthan was investigated for the first time in India. Since slaughter of cows is prohibited in India therefore management of bovine JD is critical for the success of dairy industry in the country and in this aspect the research paper is significant. Out of a total of 35 fecal samples screened by microscopy, 24 (68.5%) were positive for Mycobacterium avium subspecies paratuberculosis (MAP). Screening of 26 serum and 23 milk samples by ‘Indigenous ELISA kit’ employing semi-purified antigen of native strain (‘S 5’) of MAP, 24 (92.3%) and 14 (60.8%) were positive, respectively. Sensitivity of ‘Indigenous serum ELISA’ with reference to fecal microscopy and milk ELISA was 88.2 and 90.0%, respectively. Screening of blood samples of 14 cows, by specific PCR (IS900), 5 (35.7%) were positive. Genotyping of PCR positive HF crossbred cows using IS1311 PCR-REA showed presence of highly pathogenic ‘Indian Bison type’ genotype. Comparison of 3 tests (milk ELISA, fecal microscopy and IS900 PCR) with ‘Indigenous serum ELISA’ revealed substantial agreement between tests. Study also reported serious economic losses in terms of productivity (reduced quality and quantity of milk), reduced fertility and conception, decreased body weight and growth rate which left the farmer economy devastated due to attack of Johne’s disease in high yielding cattle herd of HF crossbred cows.
 
 
 
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