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Articles by Kiran Shahzadi
Total Records ( 2 ) for Kiran Shahzadi
  Ikram-ul-Haq , Uzma Hameed , Kiran Shahzadi , M. Mohsin Javed , Sikander Ali and M.A. Qadeer
  The present study deals with the optimization of cultural conditions for Cotton Saccharifying Activity (CSA) of cellulases by Trichoderma harzianum UM-11 using shake flask technique in 250 mL Erlenmeyer flasks. Among the different fermentation media tested, Carboxymethyl Cellulose (CMC) with Mineral Salts Solution (MSS) gave the best CSA. Cotton saccharification was optimal when 25 mL of the medium was incubated at 28°C for 96 h. The maximum CSA (0.528 U/mL/h) was found at initial pH 6.0. The level of inoculum was optimized at 5.0x107 conidia/25 mL medium.
  Ikram-ul- Haq , Kiran Shahzadi , Uzma Hameed , Muhammad Mohsin Javed and M.A. Qadeer
  The present study deals with isolation, screening and optimization of cultural conditions for biosynthesis of cellulases by Trichoderma harzianum using agricultural byproduct. Twenty different strains of Trichoderma harzianum were isolated from different soil samples by serial dilution method. Of all the strains tested, KM07 gave maximum production of cellulases by solid-state fermentation. Different agricultural byproducts such as wheat bran, wheat straw, rice bran, rice husk and soybean meal were tested for the production of cellulases and wheat bran was found to be the best substrate. The production of enzyme was significantly improved as the wheat bran was moistened with mineral salts CMC solution (ratio 1:1). The cultural conditions such as temperature 28°C and pH 6.5 were also optimized. The production of cellulases was maximum 72 h after the inoculation.
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