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Articles by Khalida Khan
Total Records ( 4 ) for Khalida Khan
  Khalida Khan and Muhammad Naeem
  The present study was conducted to investigate the quality of hen`s egg and to elaborate the contamination of toxic metals and their distribution between feed and egg. For this purpose, Toxic elements (Pb, Cd, Zn, Cu, Co, Ni, Cr and Fe) have been investigated their quality, quantity, suitability and possible health hazards. Lead, chromium and cadmium were found higher concentration and cobalt was detected below the detection limit. Atomic absorption spectroscopy technique was used for performing these tests and data of different local batches in Pakistan were compared.
  Khalida Khan , M. Naeem , M. Javed Arshed and M. Asif
  The present study was conducted to determine the isolation, identification and characterization of efficient oil degrading bacterial strains and to study the effect of different concentration of crude oil on the growth of bacterial strain. Two samples soil and water were collected from the crude oil contaminated areas for the isolation and characterization of efficient crude oil degrading strains. Sub culturing technique was employed to isolate 43 numerically dominant bacteria that had the ability to grow on 1.0% crude oil on nutrient agar plates. The isolates were then subjected to different concentrations of crude oil on nutrient agar, mineral salt agar media containing Phosphorous, Nitrogen and trace elements with glucose (PNTG) and without glucose (PER). These isolates showed rich growth on nutrient agar media along with crude oil. Out of 43 isolates 7 were able to grow up to 2.0% crude oil and were named as AA-1 to AA-7. These strains were also able to grow on mineral salt agar media with and without glucose but with different susceptibility to different concentrations of crude oil. Finally 3 prospective strains AA-1, AA-2 and AA-3 were selected for further studies. These strains exhibited good growth in PNTG containing 1.0% crude oil as evident by increase in Optical Density (OD) after every 24 h for five days. These isolated strains were identified by morphological and biochemical tests and were found to belong to genus Bacillus. These strains were subjected to shake flask transformation of crude oil in mineral salt media (PNTG) with glucose for 15 days. Marked change in crude oil colour was observed for these isolates, indicating their biodegradative ability. These isolated strains were able to use crude oil as the sole source of carbon and energy even under stressed environmental conditions. Thus these strains have bright potential for biodegradation of crude oil resulting in clean up of oil spills.
  Muhammad Naeem , Khalida Khan , Salma Rehman and Javad Iqbal
  The ground water quality of Lahore (Pakistan) has been assessed to see the suitability of ground water for domestic applications. Sixty ground water samples form shallow and deep wells were collected each during pre and post-monsoon seasons in the month of May and November 2004, respectively. Various water quality constituents pH, conductance, total dissolved solids, alkalinity, hardness, sodium, potassium, calcium, magnesium, chloride, sulphate, nitrate, phosphate and fluoride have been determined. The data was analyzed with reference to NEQS and WHO standards, ionic relationships were studied and hydro chemical facies were determined. Distribution of various constituents indicates that about 10-20% samples of the study area crosses the maximum permissible limit for TDS, hardness, calcium, magnesium, sulphate and fluoride while 20-30% samples crosses the limit for nitrate. The ground water of the study area has also been classified to study various hydro chemical processes.
  Khalida Khan , Muhammad Naeem , Muhammad Javad Arshad and Muhammad Asif
  High Pressure Liquid Chromatographic technique was employed to study caffeine contents in beverages. The samples were collected from local markets of Pakistan and determined its quality, quantity and suitability. A certified testing method for caffeine extraction was developed. Two extraction methods were used throughout this work solvent extraction and column extraction. The resulted extracts were concentrated then injected in HPLC equipped with ultra-violet detector. The quantitative results when compared with reference standard proved that column extraction is more selective then solvent extraction.
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