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Articles by Khalaf Alhussaen
Total Records ( 3 ) for Khalaf Alhussaen
  Khalaf Alhussaen , Emad I. Hussein , Khalid M. Al-Batayneh , Mahmoud Al-Khatib , Wesam Al Khateeb , Jacob H. Jacob , Mohamad A. Shatnawi , Ashraf Khashroum and Mohamed I. Hegazy
  Garlic extract is well known for its antibacterial and antifungal activity and is used to treat several plant pathogens. Pythium sp. was isolated from infected tomato seedlings grown in Jordan Valley (Jordan) and the species was identified as Pythium ultimum using morphological and molecular methods. The fungicidal activity of garlic extract with different concentrations in controlling the growth of the isolated Pythium sp. was determined in vitro. The control activity was highly dependent on Garlic extract concentration. For instance, undiluted garlic extract showed the highest control activity with no growth as compared to the biotic control without the extract whereas diluted garlic extracts 10 and 5% reduced the fungal growth to 15.5 and 41%, respectively. The results of this study show that garlic extract could successfully control Pythium ultimum on tomato seedlings and is considered as an environmentally friendly product.
  Mahmoud AL-Khatib , Mohammad Brake , Muien Qaryouti , Khalaf Alhussaen and Hussein Migdadi
  This study was conducted under green house conditions during summer growing seasons 2010 and 2011, to evaluate the response of 21 Jordanian tomato land races (accessions) against the vascular wilt fungus Fusarium oxysporum f.sp. lycopersici. Accessions were provided by the National Center for Agricultural Research and Extension (NCARE). Inoculation with the fungus was carried out by using the root dip method. Parameters considered in this study were discoloration, yellowing and fresh weight. Both discoloration and yellowing were measured, recorded according to 1-5 scale and their results ranged from 1-3.5, while fresh weight ranged from 33.1-76.5 g for treatments compared with 41.0-98.8 g for controls. Nine out of 21 studied tomato accessions were significantly different from other accessions and appeared to be resistant to the fungus under experimental conditions according to the 1-5 scale. Most of the resistant accessions were from the slow growing lines, while most of the fast growing accessions were susceptible. The study concluded that resistant accessions are promising ones to be used as root stocks for cultivated tomato varieties.
  Emad I. Hussein , Ghassan J. M. Kanan , Khalid M. Al- Batayneh , Khalaf Alhussaen , Wesam Al Khateeb , Janti Qar , Jacob H. Jacob , Riyadh Muhaidat and Mohamed I. Hegazy
  The post-harvest moulds Penicillium digitatum and Penicillium italicum are important plant pathogens and spoilage-causing molds especially against citrus fruits. If not treated, post-harvest moulds can cause enormous economic losses during storage and marketing. Therefore, more investigations are needed to examine new antifungal agents against such fungi. In this work, we aimed to evaluate the antifungal activity of some plant extracts (namely, Harmal seeds (Peganum harmala L.), cinnamon bark (Cinnamomum cassia L.) and sticky fleabane leaves (Inula viscosa L.), food preservatives (namely, sodium benzoate, sodium molybdate, ammonium heptamolybdate tetrahydrate, potassium carbonate and sodium bicarbonate) and their mixtures, i.e., plant extracts and food preservatives against P. digitatum and P. italicum. Both disc agar diffusion method and broth dilution methods was used to evaluate the antifungal activity of the plant extracts and food preservatives. Results revealed that methanolic fractions of cinnamons’ bark and sticky fleabane leaves showed the highest efficacy. MIC values of 150 and 37.5 μg mL-1 were obtained with cinnamons’ fraction against P. italicum and P. digitatum, respectively. Sodium benzoate was the most effective against tested fungal species. The obtained MIC values against P. digitatum and P. italicum were 37.5 and 75 μg mL-1, respectively. Mixtures of tested chemicals showed synergistic effects against both fungal species. Mixtures of sodium benzoate and fractions of either cinnamon or sticky fleabane reflected synergistic effects against P. italicum and antagonistic effects against P. digitatum. Inhibition zones against P. italicum ranged between 38-57 mm.
 
 
 
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