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Articles by Kh.A. El-Dougdoug
Total Records ( 5 ) for Kh.A. El-Dougdoug
  Kh.A. El-Dougdoug , A.A. Megahed , B.A. Othman , S.M. Lashin , M.A. Ibrahim and Idress Hamad Attitalla
  Systemic Acquired Resistance (SAR) could be induced in cucumber plants using different individual of seven microbial isolates against Cucumber mosaic Cucumovirus (CMV). These isolates were Bacillus subtilis, Bacillus polymyxa, Bacillus circulans, Pseudomonas putida, Pseudomonas fluorescens 2 and Pseudomonas fluorescens 8 as bacterial isolates and Trichoderma harzianum as fungal isolate. The occurrence of SAR was found by seed treatment with microbial liquid culture based on virus infectivity and the level of free and endogenous salicylic acid (SA), 14 days from CMV inoculation. Seven biotic inducers reduced CMV infectivity at range 16.6 to 39% and T. harzianum gave the highest percentage of reduction 39%. In addition, the level of total SA has been increased in treated plants, B. subtilis gave the highest level of SA 239.13 μg g-1 fresh weight (fwt) while, B. circulans gave the lowest level 70.1 μg g-1 fwt.
  Kh.A. El-Dougdoug , S.A. Ghazal , A.A. Mousa , H. Fahmy and A.R. Sofy
  This study was aimed to determined PCR product of the CP gene by electrophoresis. Three Egyptian isolates of Citrus psorosis virus (CPsV-EG), namely ARC, TB and TN were obtained from citrus cvs. Grapefruit, Balady and Navel, respectively. These isolates were differed in some of their external symptoms. The CPsV-EG isolates were detected by biological indexing, giving rise to Oak Leaf Pattern (OLP) on Dweet tangor. The three isolates were differentiated using Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay (DAS-ELISA), woody indicator plants, differential hosts, peroxidase isozymes and activity, total RNA content and Reserves Transcription-Polymerase Chain Reaction (RT-PCR). The severe isolate (ARC) gave the highest OD value (2.204) in ELISA, followed by the mild isolate (TB) (1.958) and the last latent isolate (TN) (1.669). These isolates differed also in incubation period, intensity of symptoms and response to sensitivity of woody indicator plants and differential hosts. The CPsV-EG isolates showed differences in isozymes fractions, RF value and intensity as compared with healthy plant. Results were confirmed by peroxidase activity where the level of peroxidase activity was considerably higher in ARC leaves than TB and the last TN. The total RNA content in infected leaves gave the highest content in ARC followed by TB isolate while the lowest was recorded in TN isolate. Finally, RT-PCR showed differences between CPsV-EG isolates of PCR products using specific primer (Ps66 and Ps65) where base number of coat protein gene ARC isolate 571 bp; TB isolate 529 bp and TN isolate 546 bp.
  Kh.A. El-Dougdoug , Rehab A. Dawoud , A.A. Rezk and A.R. Sofy
  Direct tissue printing on membranes has been applied on a large scale for an initial detection of CEVd, HSVd and PLMVd in fruit trees in Egypt. CEVd was detected mainly in sweet orange trees and occasionally in grapevine and mango. The principal characteristics of the disease on sweet orange trees. It was incidence with 15.4, 4.5 and 1.5%, respectively. HSVd was detected mainly in sweet orange trees and occasionally in apple, apricot, mandarin, grapevine, mango, peach, pear and plum trees with 25.2, 2.2, 7.2, 10.5, 12.4, 15.7, 65.6, 40.5 and 5.7%, respectively. The principal characteristics of the disease on sweet orange trees. PLMVd was detected mainly in peach and occasionally in apple, apricot, grapevine, mango, pear and plum with 45.0, 5.4, 2.5, 0.5, 13.5, 23.4 and 3.5% incidence. The principal characteristics of the disease on peach trees. The three viroids; CEVd, HSVd and PLMVd were detected frequently in sweet orange and peach occasionally in grapevine, pear, mango, plum and apricot in Egypt.
  A.R. Sofy , A.M. Soliman , A.A. Mousa and Kh.A. El-Dougdoug
  This study reports the characterization of Hop stunt viroid which has been isolated previously in Egypt from sweet orange infected with gummy bark disease namely citrus viroid II. The native structure of mature, circular forms of the gummy bark agent was detected by gel electrophoresis. The expected size of amplified cDNA by RT-PCR was approximately 300 bp. A phylogenetic tree of the Egyptian citrus gummy bark agent (Accession no. FJ984562) revealed 100, 99.7 and 97.8% a moderate degree of similarity to CVd-IIb (USA), CCaVd (Spain) and CCaVd (Egypt), respectively. The minimum free energy of a secondary structure for HSVd-EG-RNA was determined using its primary sequence at 37°C. The sequence appeared to fold into a rod-like structure at -122.1 kcal moL-1 while CCaVd-EG at -120.5 kcal moL-1. The five domains of the rod-like structure were determined. The sequence variations between Egyptian citrus gummy bark isolate and Egyptian citrus cachexia isolate in the pathogenic domain (P) tend to influence the pathogenicity of the HSVd-EG. Finally, the genetic diversity and evaluation of entropy power for the Egyptian citrus gummy bark agent and HSVd-citrus populations registered in GenBank, were viewed against the phylogenetic background of known CVd-II variants including the non-cachexia (CVd-IIa) and the causal agents of severe (CVd-IIb, CVd-IIc), more moderate (Ca903) and mild (Ca909).
  Kh.A. El-Dougdoug , M.F. Ghaly and M.A. Taha
  An antiviral producing Streptomyces species were isolated from soil rhizosphere in Zagazig province of Egypt. In order to identify the Streptomyces strains, morphological, physiological, biochemical and antagonism tests were performed. The Egyptian isolates of Streptomyces were found to be a species of calvus, canarius, vinaceusdrappus, nogalater and viridosporus. The Streptomyces spp. were grown in glycerol asparagine broth medium and the culture supernatants obtained were 0.45-μm filter. These isolates were tested in two experiments for their ability to control a Cucumber Mosaic Virus (CMV). In the 1st experiment, one half of leaves of Chenopodium amaranticolor were treated with Culture Filtrate (CF) followed by CMV inoculation on both halves. In the 2nd experiment, the first pair of Cucumis sativus leaves were treated CF with CMV mechanically inoculated onto one leaf, the other non-treated leaf was CMV inoculated after 7 days of treatment. In 1st experiment, CF treatment was able to considerably reduced the number of local lesion and in 2nd experiment, plants treated with CF showed variable visible viral symptoms compared with the broth media treated control 15 days post inoculation and remained symptom less throughout the study period. Such five Streptomyces species identified were able to produce an antiviral component in the culture filtrate, non phytotoxic and effective in local as well as systematically control of CMV infection.
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