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Articles by Kh.A. El- Dougdoug
Total Records ( 2 ) for Kh.A. El- Dougdoug
  A.A. Megahed , Kh.A. El- Dougdoug , B.A. Othman , S.M. Lashin , M.D. Hassanin , M.A. Ibrahim and A.R. Sofy
  Cucumber mosaic cucumovirus beet Egyptian isolate (CMV-Beet-EG) was detected serologically by DAS-ELISA using polyclonal antibodies in naturally infected sugar beet plants. The virus was isolated by single local lesion method on Chenopodium amaranticolor and then, propagated on sugar beet plants. The virus isolate was confirmed serologically by using dot and tissue printing immunoassay. The coat protein gene of CMV-Beet-EG was successfully amplified using specific primer by RT-PCR and the expected size was 820 bp. Sequence analysis cp gene revealed 97.3 and 96.4% identified similarity with CMV strains sequences published in GeneBank. The highest content of nitrogen bases was for Thymine (T) 164 (26.5%) followed by Cytosine (C) 163 (26.3%), then Adenine (A) 148 (23.9%) and Guanine (G) 144 (23.3%). The ratio of A/T and G/C were 0.902 and 0.883, the percentage of A+T and G+C was found 50.4 and 49.6. The deduced amino acids sequence of CMV-Beet-EG/CP gene was 206 amino acids with a molecular weight of 22.983 kDa and isolelectric point of 9.95. There are total 20 amino acids, the amino acid Serine (S) has the highest content and ferquency of all amino acids 25 and 12.1% while the lowest content and frequency of amino acid was 1 and 0.5% to the each of amino acid Histidine (H) and Trpyptophan (W). The CMV-Beet-EG/CP gene sequence was recorded in GeneBank with Accession No. JX826591.
  A.R. Sofy , A.A. Mousa , A.M. Soliman and Kh.A. El- Dougdoug
  Gummy bark disease is a disorder of sweet orange on sour orange rootstock in Egypt. There is an importance for hot-growing temperatures to symptom development of citrus viroids. The geographical distribution of the gummy bark disease in some world countries depend on high temperatures for viroid-symptom expression. So, correlation between climatic factors and gummy bark disease through GIS is studied. We designed a satellite map for the gummy bark disease distribution all over the world using the previous registered results. Superimposed maps of BIOCLIM annual Min-temperature, Max-Temperature and the points distribution, indicated that gummy bark disease naturally occurs in the low temperature zones range from 8-18°C at winter and from 27-38°C at summer season where the altitude ranged from -351 to 1320 m. A novel method called maximum entropy distribution modeling was used for predicting potential suitable habitat for gummy bark disease in Egypt using occurrence records. The Maxent model’s internal jackknife test of variable importance showed that altitude and mean temperature of driest quarter are the most important predictors of citrus gummy bark disease-habitat distribution.
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