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Articles by Kh. Mirzadeh
Total Records ( 11 ) for Kh. Mirzadeh
  M. Shakeri , H. Roshanfekr , M. Mamoei and Kh. Mirzadeh
  The present research studied the effect of the interval between animal`s death and sperm recovery (0, 24 or 48h) on the quality and freezability of ram spermatozoa from cauda epididymidis. Storage temperature of epididymes (room temperature or 5°C) was also analyzed. Spermatozoa were diluted with Tes-Tris-Fructose solution supplemented with egg yolk (10%) and glycerol (4%) and frozen using a programmable bio-freezer (-20°C min-1). Pre-freeze and post-thaw sperm samples showed viable spermatozoa up to 48h after the animal`s death, although their quality declined significantly as post-mortem storage time increased. Epididymes sperm stored at 5°C showed better motility and a lower percentage of abnormal forms than epididymes stored at room temperature after 24 and 48h. The fertilizing ability of cauda epididymis ram spermatozoa obtained at 0 and 24h after the animal`s death is similar to that of ejaculated spermatozoa. Therefore, a good protocol for post-mortem semen collection in rams when epididymal spermatozoa cannot be collected immediately is to preserve the epididymes at 5°C and process the samples in the first 24h after the animal`s death.
  E. Rahmatnejad , M. Bojarpour , KH. Mirzadeh , M. Chaji and T. Mohammadabadi
  This study was designed to evaluate the effect of Dried Tomato Pomace (DTP) on hematological indices of broiler chickens. Based a randomized completely design, 160 days old Ross 308 broilers were distributed into 16 floor pens and reared for 42 days. A basal diet was formulated according to NRC recommendations for starter (0-21 days) and grower (22-42 days) periods. The basal diet was also supplemented with 8, 16 and 24% dried tomato pomace, resulting 4 dietary treatments were prepared including control group. Each dietary treatment was fed ad-libitum to 4 replicates group of 10 birds at the beginning of rearing period. Inclusion of 24% DTP into the diet significantly decreased concentration of total protein, cholesterol and Low Density Lipoprotein (LDL) in serum (p<0.05). The highest High Density Lipoprotein (HDL) were recorded for birds fed diets supplemented with 16 and 24% DTP (p<0.05).
  Y. Shojaei Tabar , J. Fayazi , H. Roshanfekr , Kh. Mirzadeh and A.S. Sadr
  Prolactin is a polypeptide hormone with multiple functions, secreted mainly by the anterior pituitary gland this experiment conducted to investigate of prolactin polymorphism in buffalo population in Khuzestan of Iran. DNA was isolated from blood samples of 85 buffalos of different areas. In this study, used Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique for amplified a 156 fragment consisting of part of exon 3. The amplified fragment digested with RsaI restriction endonuclease and subjected to electrophoretic separation in ethidium bromide-strained 3% agarose gel. All the samples studied comprised of single fragment of 156 bp indicating the monomorphic nature of the locus, showing AA genotype.
  L. Ghaemnia , M. Bojarpour , Kh. Mirzadeh , M. Chaji and M. Eslami
  In this study effect of different levels of zeolite on digestibility and some blood parameters in Arabic lambs investigated. In this experiment a change-over design with eigth Arabic lambs with an average live weight of 35±2 selected. Foure rations and foure period was employed. The 4 treatments were: control groups, C+ 3%, C+ 6% and C+ 9% zeolite. The rations were fed to lambs as total mixed feed offered and orts and fecal samples were recorded daily. Blood samples were collected on the end of each period. Plasma glucose concentration was not significantly affected by treatment but tended to be lower with added zeolite. Conversely, the dietary inclusion of resulted in a lower plasma urea-N concentration (p<0.05). Intake of DM was higher for lambs receiving zeolites (p>0.05). Digestibility of DM was lowered with added zeolite (p<0.05). Digestibility of CP and NDF were increased by inclusion of 6% zeolite. This difference was significant (p<0.05) but digestibility of ADF was not significantly affected (p>0.05).
  A. Masoudi , M. Bojarpour , M. Chaji , M. Eslami and Kh. Mirzadeh
  Study was conducted to evaluate the economic value of broiler chicken diets containing Date Pits (DP) replaced by maize. About 180 days old commercial broiler chickens randomly were assigned to four experimental diets; treatments 1-4 included dietary containing 0, 10, 20 and 30% of date pits, respectively. All diets were isonitrogenous and isocaloric and formulated according to Nutrient Requirements of poultry (NRC). The results showed that in compared with control group cost (kg) of feed decreased with increasing of date pits in diets. At whole of starter and grower periods, there were no significant differences (p>0.05) in feed intake. Feed conversion ratio of diets containing 20 and 30% of date pits was significantly more than control diet (respectively, 2.44 and 2.53 vs. 1.91) (p<0.05) but there was no significant difference with diet containing 10% date pits (p>0.05). As cost (kg) of meat production there was no significant difference (p>0.05) between diets, although the cost of meat production was higher in diets containing of date pits (p>0.05). Therefore, date pits reduce the cost of diets but not meat.
  Kh. Mirzadeh , A. Masoudi , M. Chaji and M. Bojarpour
  This study was conducted to evaluate the quality of raw milk produced by the 140 dairy cow farms at different locations of Lordegan region. Solid Non Fat (SNF), Crud Protein (CP), fat, density, Dry Matter (DM), water and freezing point of samples were measured. The result showed that milk compositions of dairy farms were containing 87.42±1.13% water, 8.67±0.69% SNF and 12.57±0.69% total solid matters. Solid matters were including: 3.90±0.97% fat, 3.2±0.22% protein and 5.03% lactose and other minerals and vitamins. The data demonstrate that milk composition of Lordegan dairy farms had corresponded to global and Iran average.
  K. Mohammadi , A. Aghaei , M. Bojarpour , Kh. Mirzadeh and Gh. Motaghinia
  The aims of this study were to investigate the importance of maternal effects and to determine the most appropriate model of analyses for early growth traits of Arabi lambs. Records of 2445 lambs from 139 rams and 804 ewes for Birth Weight (BWT) on 2237 lambs from 127 rams and 784 ewes for Weaning Weight (WWT) and on 2098 lambs from 115 rams and 739 ewes for Average Daily Gain from birth to weaning (ADG) were used in this research. The data collected from Animal Science Research Station of Agricultural and Natural Resources Ramin (Khuzestan) University during 2001-2008. Genetic parameters were estimated by derivative free restricted maximum likelihood method. Six different animal models were fitted by including or excluding maternal genetic effect, maternal permanent environmental effect and covariance between direct-maternal genetic effects. On the basis of log likelihood ratio test results, Model 3 which included direct genetic and maternal genetic effects was determined to be the most appropriate model for all traits. The maternal genetic effects contributed about 74, 69 and 64% to the direct genetic effects and 15, 11 and 10% to the phenotypic variance for BWT, WWT and ADG, respectively. Depending on the model, the estimates of maternal heritability ranged from 0.074-0.146 for early growth traits of Arabi lambs. Results showed that maternal genetic effects were important for pre-weaning growth traits and should not be neglected from the model; therefore inclusion of maternal effects into the model for mentioned traits is necessary.
  S. Rahimnahal , J. Fayazi , Kh. Mirzadeh , M.T. Beigi Nassiry and H. Roshanfekr
  The Bovine Lymphocyte Antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. Since different alleles favor the binding of different peptides, DRB3 has been extensively evaluated as a candidate marker for associations with various bovine diseases and immunological traits. Therefore in this study, the genetic diversity of the bovine class II DRB3 locus in the buffalo population in Khouzestan Province of Iran investigated by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method (PCR-RFLP). Buffalo DNA isolated from whole blood. A hemi-nested PCR followed by digestion with restriction endonucleases Hae III conducted on the DNA.
  Kh. Mirzadeh , S. Tabatabaei , M. Bojarpour and M. Mamoei
  The aim of this study was comparison of hematological parameters according strain, physiological status, age, season and sex in cattle. Blood samples from 300 Holstein, Brown-swiss, Semental, Brown-swiss x Semental crossbreed and Brown-swiss x Holstein crossbreed cattle at different physiological status (dairy cows, dried cows, non pregnant cows, pregnant heifers, suckling and non suckling calf), age (0.5-3, 3.5-6, 6.5-9, 9.5-18, 18.5-36, 36.5-60 and >60 months) sex (male and female) and season (spring, summer, autumn and winter) were used. RBC mass, WBC mass, Hemoglobin concentrations (HB), PCV, MCV, MCHC, MCH, platelet mass, percent and concentration of lymphocyte, neutrophil, eosinophil and monocyte were determined with routine methods. Except for PCV, neutrophil, lymphocyte, eosinophil percents and neutrophil-lymphocyte ratio, there was not significant differences for other hematological parameters between strains. Except for Platelet concentration, monocyte percent and count, the other hematological parameters were significantly differ between various physiological status and ages of cattle. Except for Hb, PL, WBC and monocyte concentration, the other hematological parameters were significantly differ between sexes. Except for neutrophil concentration, monocyte percent and concentration, the other hematological parameters were significantly differ between seasons.
  K. Mohammadi , M. Mamouei , M. Bojarpour , Kh. Mirzadeh and A. Aghaei
  The aim of the present study was to estimate variance components and genetic parameters for post-weaning growth traits of 2634 Zandi lambs from 202 sires and 1093 dams for 6 Month Weight (6MW) of 1893 lambs from 188 sires and 816 dams for 9 Month Weight (9MW) and of 1115 lambs from 156 sires and 514 dams for Yearling Weight (YW). Data and pedigree information used in this study were collected from Khojir Research Station (Tehran-Iran) during 1993-2008. Genetic parameters were carried out by Restricted Maximum Likelihood (REML) method, under six different single-trait animal models. Log likelihood ratio test indicated the most appropriate model for 6MW should included direct additive genetic effects as well as maternal permanent environmental effects whilst the most appropriate model for 9MW and YW had only the direct additive genetic effects. The effects of lamb’s sex, dam’s age and birth year were highly significant on all three traits (p<0.01) but birth type was only significant effect on 6 MW (p<0.05) and was no significant effect on 9MW and YW (p>0.05). Average weights were 27.55±0.09, 32.67±0.11 and 34.92±0.16 kg for 6MW, 9MW and YW, respectively. Estimates of direct heritability (h2) were 0.132, 0.134 and 0.133 for 6MW, 9MW and YW, respectively. The estimate of maternal permanent environmental variance as a proportion of phenotypic variance (c2) was 0.056 for 6MW.
  B. Abbasi , J. Fayazi , M.T. Beigi Nasiri , H.A. Roshanfekr , Kh. Mirzadeh and A.S. Sadr
  Caseins are a family of milk proteins that approximately constitute 80% of total milk proteins. They are existing in several molecular forms (alpha S1, alpha S2, beta and kappa) with variant alleles of each. The A and B are the most common in cattle breeds. Allele A has threonine (ACC) and aspartic asid (GAT) amino acid at position 136 and 148, respectively by means of point mutation, isoleucine (ATC) substitutes threonine and aspartic acid is substituted by alanine (GCT) and variant A changes to variant B. In this study, the PCR-RFLP method was used for differentiating buffalo population in Khouzestan Province k-CN allels. In order to determaind the level of polymorphism, blood sample were collected from 86 buffalos in Shadegan, Ahwaz, Sosangerd, Dezfoul and Shoshtar cities. DNA extraction was based on Boom method and exon 4 of the kappa casein gene was amplified by specific set of primers for this gene to produce a 453 bp fragment. The amplified fragment were digested with Hinf I restriction endonuclease and then subjected to electrophoresis separation in ethidium bromide-stained 2.5% agarose gel. The results were revealed all buffalo samples were monomorph and genotyped as BB. In this breed, the B allele is only existed, which is reported to be favorable for milk quality and can use for breeding strategies of dairy animals.
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