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Articles by Kezong Qi
Total Records ( 2 ) for Kezong Qi
  Kezong Qi , Liu Miao , Kaisong Peng , Jian Tu and Xiuheng Xue
  Chicken Beta Defensin-8 (AvBD8) is an antibacterial peptide that plays significant roles in innate immunity. The aim of this study was to investigate the expression of Chicken avian AvBD8 mature peptide gene in Lactococcus lactis (L. lactis) system. In this study, the AvBD8 gene was cloned and sequenced from chicken liver by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Then, the AvBD8 mature peptide gene was amplified and cloned into the expression vector pNZ8048. The result showed that the recombinant vector pNZ8048-AvBD8 that verified by colony PCR, double digestion and DNA sequencing was transformed into the competent cell L. lactis NZ9000 by electroporation. The expression of the recombinant protein AvBD8 was confirmed by tricine SDS-PAGE analysis. The present findings suggest that L. lactis could act as a host for the production of avian beta defensins for further study.
  Jian Tu , Kezong Qi , Xiuheng Xue , Haixia Wang , Xueyan Wang , Kaisong Peng , Lingling Xie and Yanli Wu
  Avian β-Defensin-2 (AvBD2) is an antimicrobial peptide that plays a significant role in the innate immunity of chickens. In this research, the expression vector pPIC9K-AvBD2 was established and expressed in Pichia pastoris. AvBD2 cDNA was cloned from total RNA of chicken lung by Transcriptase-Polymerase Chain Reaction (RT-PCR). The AvBD2 mature peptide gene was amplified from and cloned into a pPIC9K plasmid. Then the recombinant expression vector pPIC9K-AvBD2 was transformed into P. pastoris GS115 to express the fusion protein. The result showed that a recombinant secretion expression vector pPIC9K-AvBD2 was successfully constructed and a fusion protein about 3.9 kDa chicken AvBD2 was expressed. Densitometric scanning analysis demonstrated that the expressed protein exaccounted for about 63.7% of the total secreted bacterial protein. The expression products had antimicrobial activity to Escherichia coli and Staphylococcus aureus in the Assay of Agar Diffusion Method.
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