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This experiment was conducted to evaluate the combined
effect of ethylenediaminetetraacetic acid (EDTA) and microbial phytase
on the concentration of minerals of serum and parameters of mineralization
of tibia in Hy-line commercial layers (W-36) in 53-64 weeks of age. One
hundred and ninty two of laying hens were tested. The experimental design
was completely randomized design with a 3x2 factorial arrangement with
three levels (0, 0.1 and 0.2%) of EDTA and two levels (0.0 and 300 FTU
kg-1) of microbial phytase in low available phosphorus diets
with 6 treatments, 4 replicates and 8 hens in each replicate. The concentration
of zinc, cupper and manganese of serum and ash, calcium and phosphorus
of tibia was evaluated. The results showed that adding of EDTA into low
available phosphorus diets significantly affected the concentration of
serum zinc and tibia ash calcium (p < 0.05). Interaction between EDTA
and microbial phytase significantly affected the concentration of copper
and manganese in serum and tibia ash percentage (p < 0.01). Using 300
FTU kg-1 microbial phytase into low available phosphorus diets
increased tibia ash phosphorus percentage (p < 0.01).
