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Articles by Kek Heng Chua
Total Records ( 3 ) for Kek Heng Chua
  Kek Heng Chua , Suzita Mohd Noor , Ching Hoong Chew and Chin Theam Lim
  Mortality rate in the preterm neonate is believed to be associated with the chronic lung disease (CLD) and CLD is mainly caused by the infections of either Ureaplasma urealyticum or Mycoplasma horminis based on other studies. Therefore in this research, endotracheal aspirates of 57 intubated Malaysian premature neonates were used to study the association between colonization of Ureaplasma urealyticum or Mycoplasma in the respiratory tract of preterm neonates with subsequent development of chronic lung disease using Mycofast® screening kit and nested PCR approaches. Overall, only 1 out of 57 samples was detected to have a mixture of 2 different Mycoplasma species using the later approach. Therefore, we conclude that development of chronic lung disease in Malaysian premature neonates is not due to colonization of Ureaplasma urealyticum or Mycoplasma but other factors.
  Kek Heng Chua , Hwa Chia Chai and S.D. Puthucheary
  This study reportsa sequential biopanning against serum obtained from a patient infected with B. pseudomallei using a phage display peptide library. A peptide bearing the consensus sequence NKNSFDAWLQSF was obtained after 3 rounds of biopanning and it partly resembles the sequence 35FDAWLAAQEF44 of B. pseudomallei site-specific recombinase, which is a phage integrase family protein. Overall, the mimotope obtained in this study could be a potential candidate for the development of a cost effective diagnostic test or vaccine in the future.
  Kek Heng Chua , Kah Heng See and S.D. Puthucheary
  Plug molds used for PFGE can be expensive to purchase and often physically and experimentally inflexible. The use of plastic primer container covers as plug molds for Pulsed-Field Gel Electrophoresis (PFGE) is reported in this study. These plastic covers are cheap, effective and easy-to-use for processing samples for electrophoresis. Besides, the broad surface and flat area in the mold promotes faster cooling and the agar sets quickly at room temperature. Plugs are also easily removed with a spatula. These containers can be easily cleaned for re-use or disposed of as waste. Twenty samples can be prepared in one container. The PFGE results obtained by using these plastic primer-container covers as plug molds are similar to the PFGE results obtained by using commercial plug molds.
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