Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Keith L. Kirkwood
Total Records ( 1 ) for Keith L. Kirkwood
  Wenpu Zhao , Min Liu and Keith L. Kirkwood
  AU-rich elements (AREs) in the 3`-untranslated region (UTR) of unstable mRNA dictate their degradation or mediate translational repression. Cell signaling through p38α MAPK is necessary for post-transcriptional regulation of many pro-inflammatory cytokines. Here, the cis-acting elements of interleukin-6 (IL-6) 3`-UTR mRNA that required p38α signaling for mRNA stability and translation were identified. Using mouse embryonic fibroblasts (MEFs) derived from p38α+/+ and p38α–/– mice, we observed that p38α is obligatory for the IL-1-induced IL-6 biosynthesis. IL-6 mRNA stability is promoted by p38α via 3`-UTR. To understand the mechanism of cis-elements regulated by p38α at post-transcriptional level, truncation of 3`-UTR and the full-length 3`-UTR with individual AUUUA motif mutation placed in gene reporter system was employed. Mutation-based screen performed in p38α+/+ and p38α–/– mouse embryonic fibroblast cells revealed that ARE1, ARE2, and ARE5 in IL-6 3`-UTR were targeted by p38α, and truncation-based screen showed that IL-6 3`-UTR-(56–173) was targeted by p38α to stable mRNA. RNA secondary structure analysis indicated that modulated reporter gene expression was consistent with predicted secondary structure changes.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility