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Articles by Kavindra Nath Tiwari
Total Records ( 5 ) for Kavindra Nath Tiwari
  Janardan Yadav , Jay Prakash Verma and Kavindra Nath Tiwari
  Aspergillus niger strain BHUAS01, Penicillium citrinum strain BHUPC01 and Trichoderma harzianum was tested for phosphate solubilizing potential and plant hormones production (indole acetic acid). The fungal strains were tested for their in-vitro phosphate solubilizing potential using Pikovskaya broth containing tricalcium phosphate (TCP) as the sole phosphorus source. All the cultures were found to solubilize TCP but with varying potential. The Aspergillus niger showed maximum amount of soluble phosphate (328 μg mL-1), followed by P. citrinum (301 μg mL-1) and T. harzianum (287 μg mL-1) after 6 days of incubation at 28±2°C. Indole Acetic Acid (IAA) production was estimated maximum in A. niger (85 μg mL-1) followed by T. harzianum (68 μg mL-1) and P. citrinum (52 μg mL-1) after 3 days of incubation at 28+2°C. T. harzianum is a well known biological controlling agent against several soil borne phytopathogens. It showed antagonistic against Fusarium oxysporum and Rhizoctonia solani. Under laboratory conditions, coinoculation of T. harzianum and A. niger showed significant increase in chickpea (Cicer arietinum) growth parameters including shoot length, root length and dry weight of shoot and root followed by single inoculation of T. harzianum and coinoculation of A. niger and P. citrinum over control. The study explores high P-solubilizing potential of A. niger and T. harzianum, which can be exploited for the solubilization of fixed phosphates present in the soil and produce IAA, thereby enhancing soil fertility and plant growth. Coinoculation of T. harzianum and A. niger could be effective biofertilizer and biocontroling agent for chickpea production.
  J.P. Verma , J. Yadav and Kavindra Nath Tiwari
  Field-based experiments were conducted to evaluate the combined application of Rhizobium sp. BHURC01 and plant growth promoting rhizobacteria on chickpea production in two consecutive years. A positive influence of plant growth promoting rhizobactria and Rhizobium sp. BHURC01 on nodulation, plant biomass, nitrogen and phosphorus in nodule, grain and straw and yield related parameter were recorded in two year of field experiments. The maximum significant increase in nodule number, dry weight of nodule, root and shoot were recorded in co-inoculation of Rhizobium sp. BHURC01 and Pseudomonas fluorescens followed by co-inoculation of Rhizobium sp. BHURC01, Azotobacter chroococcum and Bacillus megaterium over uninoculated control in two year of field study while, nitrogen and phosphorus content increase in nodules, grain and straw. The Rhizobium sp. BHURC01 and P. fluorescens showed significant increase in all parameter due to more available nitrogen by Rhizobium sp. BHURC01 and more available of phosphorus, iron and plant hormones like indole-3-acetic acid (IAA) and antifungal activity by P. fluorescens in comparison of B. megaterium and A. chroococcum. Therefore, co-inoculation of Rhizobium sp. BHURC01 and P. fluorescens could be effective biofertilizer for chickpea (Cicer arietinum L.) production.
  Jay Prakash Verma , Janardan Yadav , Kavindra Nath Tiwari , Lavakush and Vimal Singh
  Rhizospheric soils of crop plants have more flora and fauna due to availability of more organic compound, macronutrient and micronutrient. Rhizobacteria that exert beneficial effects on plant growth and development are referred to as Plant Growth Promoting Rhizobacteria (PGPR). Plant growth promoting rhizobacteria is a group of free living soil bacteria, which have ability to promote growth and yield of crop plant by direct and indirect mechanism. PGPR is generally two type, one is colonies inside plant cells that called intracellular PGPR (iPGPR) and other colonies out side plant in rhizosphere that called extracellular PGPR (ePGPR). This review generally focused on direct and indirect mechanism of PGPR. Direct mechanism of plant growth promotion may involve the synthesis of substances by the bacterium or facilitation of the uptake of nutrients from the environment. The indirect mechanism of plant growth occurs when PGPR lessen or prevent the deleterious effects of plant pathogens on plants by production of inhibitory substances or by increasing the natural resistance of the host. The search for PGPR and investigation of their mode of action are increasing at a rapid use as commercial biofertilizers. The mode of action and practical application of PGPR in crop production are the major focus of this review.
  Sunil Kumar Mishra , Bhoosan Yadav , Prabhat Upadhyay , Pradeep Kumar , Chandrashekhar Singh , Jyoti Dixit and Kavindra Nath Tiwari
  Background and Objective: Around 80% people are suffering from central nervous system (CNS) disorder and epilepsy, where most of the patients do not get adequate treatment. Management of epilepsy through synthetic drugs exhibit some side effects and may cause neurological changes. The aim of present study was to examine the Luffa cylindrica entire fruit alcoholic extract for polyphenol profiling, antioxidant and anti epileptic activity followed by biochemical and oxidative stress study and report a safe natural source for the management of epilepsy. Materials and Methods: The prepared extracts were detected in positive and negative mode of UPLC-MS/MS study followed by antioxidant activity through, 1,1-Diphenyl-2-picryl hydrazyl (DPPH) (227.19±0.84), superoxide dismutase (SOD) (2386.03±0.66) and lipid peroxidation (LPO) (226.14±1.38). The same extract was studied for diazepam induced sleep, pentylenetetrazole induced (PTZ) and maximal electroshock (MES, 70 mA) induced convulsion in wistar rat. Oxidative stress and cholinesterase activity of brain tissue was studied by estimating reduced glutathione and lipid peroxidation, total protein in both convulsive models. The changes at cellular level in rat brain histopathology were examined in microscope. Results: The study revealed the presence of total phenol (195.21±3.42 μg mL–1) and flavonoid (641.25±0.24 μg mL–1) and specifically 16 compounds i.e., hyperoside, kaempferol, acacetin, vitexin, leotiolin etc. were identified through LC-MS. The extract (400 mg kg–1) was found potentially active (p<0.05) in all the anticonvulsant study but less than the standard (phenytoin and diazepam). Minimal changes were observed in the extract (400 mg kg–1) and standard drug. Conclusion: Luffa cylindrica is using regularly in daily life as vegetable may be good source of potential antioxidant and anti epileptic activity. The alcoholic entire fruit extract showing the presence of phenolics and flavonoids suppose to be effective for the anti epileptic activity. However, further studies still needed to be carried on the extract for constituent specifically for the reported activity.
  Janardan Yadav , Jay Prakash Verma and Kavindra Nath Tiwari
  Plant Growth Promoting Fungus (PGPF) isolated from rhizosphere of chickpea and identified as Aspergillus niger strain BHUAS01 was tested for its tricalcium phosphate solubilizing ability at different sources of carbon viz., glucose, sucrose, glycerol and mannitol in Pikovskaya broth. Also, A. niger was analyzed solubilization of tricalcium phosphate (TCP) in broth media at different salinity viz., 1% NaCl, 1% KCl and 1% CaCl2 at varying range of reaction (pH) under in vitro condition. Among the carbon sources, Aspergillus niger was found to solubilize maximum tricalcium phosphate (512 μg mL-1) at glucose as carbon source and minimum activity (348 μg mL-1) of phosphate solubilization at sucrose as carbon. Aspergillus niger showed maximum significant solubilization of tricalcium phosphate in Pikovaskaya broth containing carbon source glucose followed by glycerol, maltose and sucrose at 21 days of incubation. Further the effect of different salinity (1% NaCl, 1% KCl and 1% CaCl2) was tested at different pH (6.0, 7.0 and 8.0) under in vitro condition. A. niger strain BHUAS01 was showed maximum significant solubilization of tricalcium phosphate (495 μg mL-1) in presence of 1% CaCl2 in modified Pikovskaya broth at pH 8.0 than other salt concentration. This finding can provide great benefit in the maintaining the available phosphates for crops in saline and alkaline soils. A large fraction of land arid and semiarid regions is affected by salinity in India.
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