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Articles by Katarzyna Chojnacka
Total Records ( 11 ) for Katarzyna Chojnacka
  Katarzyna Chojnacka
  The paper presents the results of studies on kinetics and equilibrium of bioaccumulation process of Cr(III) ions by blue-green alga Spirulina sp. Bioaccumulation was described as the process that consists of two stages, passive (identical with biosorption) and active (accumulation inside the cells). The passive stage (similarly as biosorption itself) was found to be quick process and the subsequent active stage (accumulation) was much slower and required metabolic activity of cells. The overall equilibrium of bioaccumulation process was reached after ca. 30 hours. The efficiency of bioaccumulation was compared with biosorption performance of cells grown under the same conditions. Two applications of bioaccumulation were discussed: metal ions removal from wastewater and metal ions binding to the biomass to produce biological feed supplement with microelements. The experimental results showed that bioaccumulation capacity was greater than biosorption capacity. Wastewater treated by the process of bioaccumulation contained lower residual metal ions concentration due to equilibrium shift of external (biosorptive) metal ions binding capacity towards lower values, resulting in lower residual metal ions concentration and thus higher treatment efficiencies. If bioaccumulation was considered as the method of biomass enrichment to produce mineral feed supplements for livestock, bioaccumulation enabled to bind more microelements, not only to the surface of cell wall, but also to accumulate inside the cells.
  Zbigniew Dobrzanski , Henryk Gorecki , Katarzyna Chojnacka , Helena Gorecka and Marzena Synowiec
  Mineral-humic preparations Humokarbowit (HKW) and Humobentofet (HBF) were used in feeding of reproductive laying hens Lohmann Brown. The effect of these preparations on microelement composition of eggs was investigated. The content of microelements in hens diet, egg albumen and eggshell was determined by ICP-MS technique and relative bioaccumulation coefficient (RBC) was evaluated. The humic preparations significantly increased selenium and decreased molybdenum concentration in egg albumen. Increase of selenium and iron content in egg yolk and increase of iron concentration in eggshells was observed. The preparations did not influence the content of such elements as Cr, Co, Cu, I, Mn, Zn. The highest levels of RBC were found for selenium and iodine and the lowest for manganese.
  Helena Gorecka , Henryk Gorecki , Katarzyna Chojnacka , Marzanna Baranska , Izabela Michalak and Agnieszka Zielinska
  The paper discusses the possibility of using renewable sources: slaughter wastes and wood ashes in the production of NPK fertilizers. The content of macronutrients, micronutrients and toxic elements in these materials was reported. In the present work, poultry feathers were used as fertilizer source of nitrogen, animal bones as the source of phosphorus and wood ash as the source of potassium and micronutrients. Bioavailability of fertilization components was increased by mineralization with sulfuric acid, which transformed keratinous nitrogen of poultry feathers into ammonia nitrogen, hydroxyapatite phosphorus to bioavailable orthophosphate. Also, mineralization of organic content of the materials was achieved. The method of production and the composition of NPKS fertilizer, the content of which was adjusted to the requirements of oil seed rape was provided. The fertilization properties were checked in germination tests.
  Daniel Korniewicz , Zbigniew Dobrzanski , Katarzyna Chojnacka , Adolf Korniewicz and Roman Kolacz
  Yeasts Saccharomyces cerevisiae containing in 1 kg of dry product, separately 32.0 g Fe (Y-Fe), 23.0 g Cu (Y-Cu), 35.4 g Mn (Y-Mn), were used as the source of these microelements in complete diet for growing pigs. If considering livestock requirements, feed supplementation with this organic form of microelements, covered animals’ requirements in: Fe-27%, Cu-75%, Mn-30%. ZnO was the source of zinc. The experiments were carried out on 25 barrows. Digestibility of the main nutrients, the apparent absorption and balance for Fe, Cu, Mn as well as Zn, Ca, P, Mg, were carried out. The supplemented microelements did not have the adverse effect on digestibility of the main nutrients and the apparent absorption of Ca, P and Mg. Barrows fed with feed containing Y-Fe excreted more iron with urine than the control, which statistically significantly (P<0.05) influenced decrease of retention from 67.4 to 58.0 %. Absorption was similar in both groups. The supplementation of Y-Cu had the influence on increase of retention of Cu up to 25.6 % and absorption up to 28.1 % when compared with the control group (21.3 and 23.7 %), respectively. Supplementation of Y-Mn also significantly (P<0.05) increased Mn retention up to 20.1 % and absorption up to 21.4 % when compared with the control group (12.8 and 13.8 %, respectively). Joint supplementation of Y-Fe, Y-Cu and Y-Mn did not improve retention and absorption of all the microelements. In the group Y-Cu, significant (P<0.05) decrease of retention and absorption of Zn was observed when compared with the remaining groups. It was found that in pigs feeding, yeasts enriched with copper and manganese may find application in practice. However, yeasts enriched with iron, were found to be less efficient than inorganic form of this bioelement.
  Izabela Michalak , Agnieszka Zielinska , Katarzyna Chojnacka and Jan Matula
  Equilibrium experiments of chromium(III) biosorption by microalga (Spirulina sp.) and macroalga (Pithophora varia) were investigated in this paper. Influence of the following process parameters was studied: pH, biomass concentration and temperature. The equilibrium of the process was described with Langmuir equation. The model parameters were determined at various process conditions. The best operation conditions for the process were selected: pH 5, T 20oC, Cs 1g.l-1 for Pithophora varia and the same for Spirulina sp. Under these conditions, the maximum biosorption capacity 60.6 mg.g-1 and 34.6 mg.g-1, and affinity constant 0.182 l.mg-1 and 0.0553 l.mg-1 were reached for Pithophora varia and Spirulina sp., respectively. It was found that Pithophora varia was more efficient in biosorption of Cr(III) ions than Spirulina sp. Biosorptive properties of microalgal and macroalgal biomass can be employed either in the production of biological feed supplements for livestock or in wastewater treatment processes.
  Katarzyna Chojnacka and Piotr M. Wojciechowski
  In the present paper bioaccumulation of Cr(III) ions by blue-green algae Spirulina sp. is discussed. We found that the process consisted of two stages: passive in which Cr(III) ions are bound to the surface of cells, identical with biosorption and active, metabolism-dependent, in which Cr(III) ions are transported into the cellular interior. The passive stage occurs in both living and non-living cells and the active – only in living biomass. Two distinctive mathematical models of the process were proposed. The first was physical model basing on the identified mechanism of the process. In the second model, artificial neural networks were proposed.
  Izabela Michalak , Katarzyna Chojnacka and Peter Glavic
  Problem statement: The idea of the application of biological material as mineral feed additives could constitute an innovative practice that would encourage environmental sustainability. The main idea of this study was to present the advantages of macroalgae enriched with microelements when compared to inorganic salts. Approach: In order to evaluate the potential participation of macroalgae in sustainable animal feeding, it was necessary to consider several concepts, such as: Waste Minimization (WM), Cleaner Production (CP) and Pollution Prevention (PP), which were closely related to sustainable production. Special attention was also paid to the prevention of waste generation during production process of mineral feed additives from macroalgae. Results: This kind of feed additives could contribute to the minimization of nutrient excretion by animals, by optimizing nutrient availability and proportion in the animal diet. Conclusion: The application of macroalgae in animal feeding could be considered as preventive environmental strategy, which would reduce the risks of the excess of microelements in the environment and in the animal diet.
  Katarzyna Chojnacka and Facundo-Joaquin Marquez-Rocha
  Some microalgae can grow metabolizing inorganic and organic carbon sources, which might occur simultaneously and independently, while energy is supplied by light and/or an organic carbon source. In this context, the contribution of each metabolism to total growth can be determined by quantitative analysis. The illumination of microalgal cells growing in the presence of organic substances, might cause an effect which can drive the carbon metabolism in different ways. When analyzing the growth of different strains of microalgae, some differences could be distinguished, between additive or inhibitory effect of light on heterotrophic metabolism in mixotrophic or photoheterotrophic growth. This manuscript proposes, the integration of a kinetic and stoichiometric metabolic model which explains the differences of carbon and energy utilization modes between mixotrophic and photoheterotrophic growth in microalgae. This model presumably discloses relevant independent facts between the mechanisms of photosynthesis and the oxidative metabolism of organic compounds, such as glucose and the importance of these differences on the production of biomass and secondary metabolites.
  Izabela Michalak and Katarzyna Chojnacka
  The aim of this article was to discuss the mineral composition of marine edible macroalga Enteromorpha spp. sampled from the coast of the Gulf of Gdansk and from the open Baltic Sea, with the consideration of its use both as biomonitor and also as a future feed additive for animals and food for humans. The mineral content of Baltic seaweed was determined by the multielemental analysis by ICP-OES Vista-MPX instrument from Varian (Australia). The results indicated geographical and seasonal differences between concentrations of elements. The content of microelements in all examined macroalgae was at a rather steady level. Significant fluctuations were observed in the content of macroelements - generally, Enteromorpha spp. from the southern Baltic contained more alkali (Na, K) and alkaline earth metals (Ba, Mg, Ca) than macroalga from the Gulf of Gdansk. In order to determine the degree of accumulation of each element by the green alga, concentration factors (CFs) with respect to seawater were calculated. This factor for Fe, Al, Cu, Mn and Ti in algae was much higher, than for K, Ca, Mg and Na. The composition of Enteromorpha spp. was also compared with grains (corn, spring wheat, spring triticale, spring barley, oat, rye), which are commonly used as feed material. Macroalgae contained on average 56 times more Na(I), 11 times more Mg(II), 8 times more Ca(II), 5 times more K(I) and 3 times more Cu(II) than the grains mentioned previously. The content of Mn(II) was 20% higher.
  Maciej Janeczek , Katarzyna Chojnacka , Nezir Yasar Toker , Ewa Pecka , Albert Czerski , Zuzanna Witkowska , Aleksander Chroszcz , Wojciech Zawadzki and Mariusz Korczynski
  Trace element additives with Cu (II), Fe (III) and Cr (II) were tested in vitro in a Hen Caecum Model. The levels of Short-Chain Fatty Acid (SCFA) were estimated in the 4th and 6th h of fermentation. Measurements of hydrogen and methane as fermentation end products were conducted. Any negative influence on the fermentation process were noted. The study observed a higher production of SCFA in the experimental group than in the control group. In the experiment an increase in propionate acid was observed except in the control and Cr (II) groups. It seems to be true that fermentation in experimental groups was more intensive and faster than in the control one. A result of intensive fermentation was a pH decrease in experimental groups but this was the within range value. The lack of a correlation was shown between the level of acetate and methane and hydrogen concentration.
  Maciej Janeczek , Katarzyna Chojnacka , Nezir Yasar Toker , Ewa Pecka , Albert Czerski , Zuzanna Witkowska , Aleksander Chroszcz , Wojciech Zawadzki and Sebastian Opalinski
  Soybean meal was enriched with Zn (II) ions via biosorption using inorganic salt (ZnSO4.7H2O). Biosorption was conducted using a column reactor with a bed of a volume of 0.1 dm3. The biosorption process was conducted at a temperature of 20°C to bed saturation, controlling the concentration of solution flowing out of the column. The enriched biomass was air dried for 48 h. The control group (C) and two experimental groups (I and II) were distinguished. The availability of zinc from the preparation obtained via biosorption method was examined in the first group (I) while the availability of zinc in a from organic chelate was assessed in the second group (II). The caecum was collected during dissection and the ingesta was obtained from it. After incubation, the gas contained in the serum bottles was subjected to analysis in order to examine bacteria activity in the ingesta. The analysis of the produced hydrogen and methane were conducted using gas chromatography method on a gas chromatograph. The samples of liquid ingesta were subjected to analysis using a gas chromatograph (Agilent Technologies 7890A GC System) with an FID detector in order to determine total SCFA concentration and the percentage contribution of particular acids: acetic, propionic, isobutyric, butyric, isovaleric and valeric. The results analysis proved no negative influence of soybean meal enriched with zinc using biosorption method on in vitro fermentation process in caecum.
 
 
 
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