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Articles by Kamyar Shameli
Total Records ( 6 ) for Kamyar Shameli
  Mansor Bin Ahmad , Kamyar Shameli , Majid Darroudi , Wan Md Zin Wan Yunus and Nor Azowa Ibrahim
  Problem statement: Silver Nanoparticles (Ag-NPs) have been synthesized by using chemical reduction method into the interlayer space of a Montmorillonite (MMT) as a solid support which is used to antibacterial application and polymer nanocomposites for fabrication of medical devices. Approach: AgNO3 and NaBH4 were used as a silver precursor and reducing agent, respectively. The properties of Ag/MMT nanocomposites were studied as a function of the AgNO3 concentration. The crystalline structure, d-spacing of interlayer of MMT, the size distributions and surface plasmon resonance of synthesized Ag-NPs were characterized using Powder X-Ray Diffraction (PXRD), Transmission Electron Microscopy (TEM) and UV-vis spectroscopy. Results: The results obtained from UV-vis spectroscopy of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks were increased with the increasing in the AgNO3 concentration. The obtained information from UV-vis spectra of Ag-NPs was in an excellent agreement with the obtained microstructures studies performed by Transmission Electron Microscopy (TEM) and their size distributions. The prepared Ag/MMT nanocomposites are very stable over a long period of time in aqueous solution. Conclusion: The synthesized Ag/MMT nanocomposites are very stable in aqueous solution over a long period of time without any sign of precipitation. Silver nanoparticles in MMT suspension could be suitable to use antibacterial applications, since MMT is viewed as ecologically and environmentally inert material and used for biological application such as cosmetics and pharmaceutical usage.
  Mansor Bin Ahmad , Kamyar Shameli , Majid Darroudi , Wan Md Zin Wan Yunus and Nor Azowa Ibrahim
  Problem statement: Silver/Montmorillonite/Chitosan Bionanocomposites (Ag/MMT/Cts BNCs) have been synthesized by UV-irradiation reduction method in the absence of any reducing agent or heat treatment which is used to antibacterial application and medical devices. Approach: MMT, Chitosan and AgNO3 were used as a solid support, stabilizer and silver precursor, respectively. The properties of Ag/MMT/Cts BNCs were studied as a function of UV-irradiation times. The crystalline structure, d-spacing of interlayer of MMT, the size distributions and surface plasmon resonance of synthesized silver nanoparticles (Ag-NPs) were characterized using Powder X-Ray Diffraction (PXRD), Transmission Electron Microscopy (TEM) and UV-vis spectroscopy. The functional groups of prepared BNCs were also determined by Fourier Transform Infrared (FT-IR). Results: The results obtained from UV-vis spectroscopy of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks were increased with the increasing in the UV-irradiation times. Results from UV-visible spectroscopy and Transmission Electron Microscopy (TEM) microphotographs show that particles size of Ag-NPs decrease with the increase of UV-irradiation time. Conclusion: UV-irradiation disintegrated the Ag-NPs into smaller size until a relatively stable size and size distribution were achieved. Ag/MMT/Cts BNCs could be suitable to antimicrobial applications and medical devices.
  Nurulbahiyah Ahmad Khairudin , Kamyar Shameli , Mikio Miyake and Nur Shima Fadhilah Mazlan
  Paenibacillus polymxca β-glucosidase B (BglB) which belongs to GH family 1 is a monomeric enzyme that acts as an exo-β-glucosidase hydrolyzing cellobiose and cello oligosaccharides of higher degree polymerization by cleaving the β-1, 4 glycosidic linkage between glucosyl residues. This study is based on the binding modes of cellopentaose consisting of five glucosyl residues in the active site of BglB. A Molecular Dynamics (MD) simulation was performed at 300 K under periodic boundary condition for 5 ns using the complex structures obtained from previous reported docking study. The residues that are responsible for recognizing the glycone and aglycone subsites include Gln22, Glu167, Glu409, Glu356, Tyr298, Trp410 and Glu225, Tyr169, Asn223, Trp412, His181, Ala360, Arg243, Gln316, respectively. The findings of this study support the fact that the interaction of subsite-1 and OH is the most crucial in the enzyme-substrate complex 2 stabilization.
  Mansor Bin Ahmad , Kamyar Shameli , Majid Darroudi , Wan Md Zin Wan Yunus , Nor Azowa Ibrahim , Azizah Abdul Hamid and Mohsen Zargar
  Silver nanoparticles (Ag-NPs) were successfully synthesized into the interlayer space of Montmorillonite (MMT) by chemical reduction method. AgNO3 and NaBH4 were used as a silver precursor and reducing agent, respectively. The properties of Ag/MMT nanocomposites were studied as a function of the AgNO3 concentration. The UV-vis spectra of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks increased with increasing AgNO3 concentration. The crystalline structure of the Ag-NPs and basal spacing of MMT and Ag/MMT were also studied by Powder X-Ray Diffraction (PXRD). The antibacterial activity of Ag-NPs was investigated against gram-negative bacteria (Escherichia coli, Escherichia coli O157:H7 and K. pneumonia) and gram-positive bacterium (Staphylococcus aureus) by disk diffusion method using Muller-Hinton Agar (MHA) at different sizes of Ag-NPs. The smaller Ag-NPs were found to have significantly higher antibacterial activity. These results showed that Ag-NPs can be used as effective growth inhibitors in different biological systems, making them applicable to medical applications such as in surgical devices.
  Mansor Bin Ahmad , Majid Darroudi , Kamyar Shameli , Abdul Halim Abdullah , Nor Azowa Ibrahim , Azizah Abdul Hamid and Mohsen Zargar
  Silver nanoparticles (Ag-NPs) were successfully synthesized into the interlayer space of Montmorillonite (MMT) by UV-irradiation method. AgNO3 and Montmorillonite (MMT) were used as a silver precursor and solid support, respectively. The properties of Ag/MMT nanocomposites were studied as a function of UV-irradiation time. UV-irradiation disintegrated the Ag NPs into smaller size until a relatively stable size and size distribution were achieved. The UV-vis spectra of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks increased with increasing UV-irradiation time. The synthesized Ag/MMT nanocomposites under longer UV irradiation time are very stable over a long time in aqueous solution without any sign of agglomeration or precipitants. The crystalline structure of the Ag-NPs and basal spacing of MMT and Ag/MMT were also studied by Powder X-Ray Diffraction (PXRD). The antibacterial activity of Ag-NPs was investigated against Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) and Gram-positive bacteria (Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA)) by disk diffusion method using Muller-Hinton Agar (MHA) at different sizes of Ag-NPs. All of synthesized Ag-NPs were found to have high antibacterial activity. These results showed that Ag-NPs can be useful in different biomedical research and applications such as diagnostic and surgical devices, contrast agents, drug delivery vehicles and physical therapy applications.
  Mansor Bin Ahmad , Kamyar Shameli , Majid Darroudi , Wan Md Zin Wan Yunus , Nor Azowa Ibrahim , Azizah Abdul Hamid and Mohsen Zargar
  A simple synthesis of silver/montmorillonite/chitosan (Ag/MMT/Cts) Bionanocomposites (BNCs) with controllable sizes is presented. In this research, reduction process of silver cations by UV-irradiation was performed. AgNO3, MMT and chitosan were used as a silver precursor, solid support and stabilizer, respectively. The properties of Ag/MMT/Cts BNCs were studied as a function of UV-irradiation time. We found that UV-irradiation disintegrated the silver nanoparticles (Ag-NPs) into smaller size until a relatively stable size and size distribution were achieved. The average size, size distribution and crystalline structure of Ag-NPs were determined by Transmission Electron Microscopy (TEM) and Powder X-Ray Diffraction (PXRD). The antibacterial activity of Ag-NPs was investigated against Gram-negative bacterium (Escherichia coli (E. coli)) and Gram-positive bacteria (Staphylococcus aureus (S. aureus) and Methicillin-Resistant Staphylococcus Aureus (MRSA)) by disk diffusion method using Muller-Hinton Agar (MHA) at different sizes of Ag-NPs. All of synthesized Ag/MMT/Cts BNCs were found to have high antibacterial activity. These results showed that Ag/MMT/Cts BNCs can be useful in different biological research and biomedical applications such as surgical devices and drug delivery vehicles.
 
 
 
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