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Articles by Kalpesh Ishnava
Total Records ( 2 ) for Kalpesh Ishnava
  Kalpesh Ishnava and J.S.S. Mohan
  Genetic diversity of 25 accessions of the medicinal climber Tinospora cordifolia was measured by isozymes from Gujarat. The germplasm was reared in a field plot under identical conditions and mature stems were used for the presented study. Analysis by using battery of ten isozymes revealed the presence of 16 gene loci and 33 alleles in 25 accessions. The percentage of polymorphic loci (P) was 45.0% and mean observed number of alleles per locus (A) was 1.57. The average observed heterozygosity (Ho) and expected heterozygosity (He) were 0.443 and 0.270, respectively shows high levels of genetic variation among different accessions. The UPGMA dendrogram clearly depict the spectra of genetic diversity among various accessions. The clustering of accession TC-1 (Kheda) and TC-2 (Songhad) appeared at the top of the dendrogram which are genetically rich. These accessions should be conserved for future breeding programme.
  Daxa Rajput and Kalpesh Ishnava
  Background and Objective: Today, dental caries is still one of the most common diseases in the world. The causes of tooth decay in most cases are the poor oral hygiene and high sugar consumption. Despite several dental caries agents being available in the market, the search for an effective agent still continues. Several undesirable side effects associated with these agents stimulated the search for alternate agents. The objective of this study was than to investigate the inhibitory effect of the crude seed extracts from some traditional medicinal plants on anticariogenic organisms under in vitro condition for their use for controlling dental caries. Attempt was also made to characterize bioactive compound at primary level. Materials and Methods: The anticariogenic activity was assessed by agar well diffusion method. MIC against six dental microorganisms and the radial zone of inhibition was measured. Phytochemical evaluation of all the selected plants showed good MIC values and TLC-Bioautography. The data was statistically analyzed by one way analysis of variance (ANOVA) using SPSS ver. 16.0. Results: The results revealed that the hexane extract of plant seeds has more potent anticariogenic activities against oral microorganisms. Maximum zone of inhibition (18 mm) was found when seed extracts of Mimusops elengi L. tested against Candida albicans. The MIC of hexane extract of Mimusops elengi L. and Punica granatum L. seed against CA and SP was 0.075 and 0.3, respectively. The MIC value of 0.3 was found for SP using Punica granatum L. The result of bioautography showed that the compound was active against Candida albicans and Streptococcus pyogenes. Conclusion: Hence, the study concludes that the very good activity of methanol extract of seed of Mimusops elengi L. and Punica granatum L. will be useful in the future development of effective toothpaste or mouth washer against oral microorganisms.
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