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Articles by Kai-Yu Wang
Total Records ( 3 ) for Kai-Yu Wang
  Kai-Yu Wang , Hai Lian , De-Fang Chen , Jin-Lu Huang , Jun Wang and Qiao-Feng Mou
  The p1 gene of Yersinia ruckeri (yrp1) which was isolated from channel catfish was amplified by PCR with specific primers and inserted into pMD19-T vector. The positive recombinant plasmid was selected and sequenced. Molecular characteristics analysis of the yrp1 gene and the protein which is encoded by it was performed. The results showed that the yrp1 gene is 1434 bp in length with G+C content of 44.35%. The analysis of codon bias indicated that the codon usage frequency of the yrp1 gene is distinctly different and it is preferable to perform in E. coli and yeast. The theoretical relative molecular mass and iso-electric point of the Yrp1 amino acid sequence are about 51.5 kDa and 4.48 separately. The polypeptide has some important sites related to post-translational modification, including 35 potential phosphorylation sites and 4 potential N-glycosylation sites. The polypeptide analyzed in this study contains a ZnMc superfamily conserved domains and does not contain a signal peptide, even though it is a secretory protein.
  Zong-Jun Du , Xiao-Li Huang , De-Fang Chen , Kai-Yu Wang and Yong-Qiang Deng
  Skin Ulcer Disease (SUD) is a new disease in Schizothorax prenati. The purpose of this study was to elucidate the etiology of SUD in recent outbreaks in Sichuan province, China. One dominant bacteria (D060501) was isolated from the diseased Schizothorax prenanti with typical skin ulcer. It was identified as Aeromonas hydrophila by morphological features, physiological and biochemical characteristics and 16S rDNA sequence. The bacterium was very sensitive to chloramphenicol and gentamicin and resistant to tetracycline, SMZ, doxycycline and carbenicillin. The virulence of the bacteria to Schizothorax prenanti was checked by challenged experiment.
  Hai Lian , Kai-Yu Wang , De-Fang Chen , Jun Wang , Ling-Yuan Huang and Cheng-Wei Li
  The p1 gene of Yersinia ruckeri (yrp1) which was isolated from channel catfish was amplified by PCR with specific primers and inserted into pMD19-T vector. The positive recombinant plasmid was selected and sequenced. Then, the yrp1 gene was subcloned into pET-32a (+) vector and transformed into BL21 (DE3) followed by induction with IPTG and detection with SDS-PAGE. Optimization of the induction conditions were conducted. The results showed that the recombinant protein with a molecular mass of about 72 kDa was mostly packaged into inclusion bodies. The optimization of induction process conditions led us to perform the fusion protein induction at 37°C for 4 h with 0.8 mM IPTG.
 
 
 
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