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Articles by K.V. Mohanan
Total Records ( 3 ) for K.V. Mohanan
  E. Abhilash Joseph , V.V. Radhakrishnan and K.V. Mohanan
  Rice plants respond to salinity stress in different ways and accumulation of phenolic compounds is one of them. An experiment was carried out presently to analyze the variation of accumulation of phenolics in some native rice cultivars of North Kerala, India in response to salt stress. Five cultivars namely Orthadian, Orkazhama, Kuthiru, Kuttusan and Chovvarian collected from one of the saline rice tracts of Kerala and two cultivars namely Kunhutty and Veliyan collected from one of the non-saline rice tracts of Kerala were used for the study. The plants were treated with gradually increasing concentrations of NaCl varying from 0-200 mM starting from the 45th day of growth onwards mimicking the gradually increasing salt content of the salinity prone rice farms of the study area and the quantity of total phenolics was investigated in each case. The cultivars collected from both the saline and non-saline rice tracts showed gradual increase in the accumulation of total phenolics in response to increase in salt stress. The cultivars showed differential variation in the accumulation of total phenolics in relation to salt stress showing that the character was cultivar specific. Among the cultivars studied, Kuthiru showed the highest quantity of phenolic compounds and Orkazhama showed the highest percentage of increase of phenolics over the control plants in relation to increase in salt stress. Salt stress tolerant cultivars can be further screened for the production of higher quantity of phenolics in response to salt stress with an objective of improving the antioxidant production potential of such cultivars.
  P.K. Usha , Sailas Benjamin , K.V. Mohanan and A.V. Raghu
  An efficient protocol was established for in vitro shoot multiplication from shoot tip explants of Vitex negundo on Murashige and Skoog (MS) basal medium supplemented with 8.87 μM 6-benzylaminopurine (BA). Inclusion of 8-Naphthalene Acetic Acid (NAA) in the culture medium along with BA promoted higher rates of shoot multiplication than BA alone. The rate shoot multiplication (6.3) after 4 week of culture on MS basal medium supplemented with 8.87 μM BA, 2.69 μM NAA. The elongated shoots rooted in half strength MS basal salts supplemented with 4.90 μM IBA + 2.85 μm IAA and 2% (w/v) sucrose. The presence of Activated Charcoal (AC) with IBA showed positive response to rooting. In vitro propagated plants were transferred to soil with a survival rate of 85% after 1 month.
  A.V. Raghu , S.P. Geetha , Gerald Martin , Indira Balachandran , P.N. Ravindran and K.V. Mohanan
  An efficient and rapid in vitro clonal propagation of the endangered medicinal tree Aegle marmelos (L.) Corr. (Rutaceae) by enhanced axillary shoot proliferation from mature single node was designed. The explants showed marked seasonal variation in their response under in vitro conditions. Explants collected in October (72.8%) and November (78.6%) showed maximum response. Multiple shoots were formed on Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 6-Benzyladenine (BA). An average of 6.2 shoots/explant could be obtained after 45 days of culture. The number of shoots was increased at the third subculture with an average of 16.3 shoots per explant. The effect of subsequent subcultures (upto 20 cycles) on shoot formation was also studied. Subculturing was carried out every 45 days on fresh shoot multiplication medium. Continuous culture in the same medium resulted in distorted and vitrified shoots. Transfer of cultures to half strength MS medium devoid of ammonium ions and cytokinin (BA) for a single cycle before going to the shoot multiplication medium could solve this problem. In vitro rooting was inconsistent in medium with different auxins (Indole 3-butyric acid-IBA, Indole 3-acetic acid-IAA and α-naphthalene acetic acid-NAA) at varying concentration and combinations. But in vitro raised shoots could be rooted ex vitro by pulse treatment with naphthoxy acetic acid (NOA) and IBA and then in chlorogenic acid followed by planting in moist sand. This treatment resulted in 83.9% survival of plantlets. The method standardised could be used for large scale planting material production and conservation of this important endangered medicinal tree.
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