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Articles by K.V. Mallaiah
Total Records ( 5 ) for K.V. Mallaiah
  M. Sridevi and K.V. Mallaiah
  Twenty Rhizobium species were isolated from root nodules of 20 legume hosts and studied for their ability to produce bacteriocins. Among the 20 species, the Rhizobium sp. isolated from root nodules of Crotalaria alata, C. juncea and C. laburnifolia produced bacteriocins after 72 h of growth. It was observed that Rhizobium sp. from C. alata produced small type and highly effective bacteriocin against Rhizobium sp. from Arachis hypogaea, Cajanus cajan, Clitorea ternatea, Dolichos lablab, Indigofera linnaei and Vigna mungo. The bacteriocin was isolated and partially purified by chloroform extraction and ammonium sulphate fractionation. The bacteriocin was sensitive to protease and insensitive to DNase and RNase. It was stable up to temperature 80 °C for 15 min. The bacteriocin fraction consistently migrated as a 29 kDa polypeptide on SDS-PAGE.
  M. Sridevi and K.V. Mallaiah
  Twenty six Rhizobium strains isolated from root nodules of Sesbania sesban (L.) Merr. were screened for their ability to produce siderophores. Among the twenty six strains, twenty strains produced hydroxamate-type of siderophores in Fiss-glucose mineral medium. The maximum amount of siderophore was produced by Rhizobium strain 22. The production of siderophore started at 8 h and reached maximum after 24 h. The addition of limited amount of iron in the media increased growth as well as siderophore production. Fiss-glucose mineral medium supplemented with 1% sucrose and 0.1% (NH4)2SO4 increased siderophore production. UV-spectrophotometric scanning of purified siderophore showed absorption maxima in the range of 500-520 nm and indicating that it belongs to dihydroxamate-type of siderophore. Tryptophan and tyrosine were identified as conjugated amino acids of the purified siderophore. The outer membrane protein profiles of the Rhizobium strain 22 grown in high iron containing medium revealed an unique protein band of molecular mass 45 kDa.
  M. Sridevi , K.V. Mallaiah and N.C.S. Yadav
  Rhizobium species isolated from Crotalaria species (C. junceaC. laburnifoliaC. retusa and C. verrucosa) were tested for their ability to solubilize tricalcium phosphate (TCP). Among these four species, Rhizobium sp. isolated from Crotalaria retusa and C. verrucosa showed zone of TCP solubilization on Pikovskaya`s agar medium. Highest solubilization index (2.70) was observed in Rhizobium sp. from C. retusa. Effect of different carbon and nitrogen sources was also studied. Among the carbon sources, glucose was found to be best carbon source for TCP solubilization. Presence of highest concentration (3%) of glucose enhanced TCP solubilization. Among the nitrogen sources, ammonium sulphate was the best promoter for the process. Addition of low levels (0.5µg mL-1) of EDTA increased TCP solubilization.
  M. Sridevi , N.C.S. Yadav and K.V. Mallaiah
  Cultural and nutritional conditions were optimized for Indole Acetic Acid (IAA) production by Rhizobium spp. isolated from root nodules of Crotalaria juncea, C. retusa, C. laburnifolia, C. verrucosa and C. alata. The isolates produced maximum amount of IAA after 72 h of incubation and at 2.5 mg mL-1 L-tryptophan concentration. The effect of different carbon and nitrogen sources on IAA production were also studied and it revealed that, mannitol and L-glutamic acid were the best promoters for IAA production. Addition of cell wall affecting agents increased the IAA production over controls. Among the five isolates of Crotalaria species, maximum amount of IAA was produced by isolate from C. retusa. The compound from Rhizobium sp. from C. retusa was extracted, purified and structurally confirmed as IAA.
  M. Sridevi , K.G. Kumar and K.V. Mallaiah
  The Rhizobium sp. isolated from stem nodules of Sesbania procumbens (Roxb.) W and A was studied for its ability to produce siderophores on Chrome-Azurol S agar medium. The symbiont was able to produce catechol-type of siderophores in culture after 4 h of incubation. Maximum siderophore production was observed after 24 h. Carbon and nitrogen sources greatly influence the siderophore production. Among the carbon and nitrogen sources, mannitol (2%) and glutamine (0.1%) were found to increase the siderophore production. Thin Layer Chromatography (TLC) of the siderophore extract showed the presence of 2, 3-Dihydroxy Benzoic Acid (DHBA) and 3, 5-DHBA. Arginine, glutamine and proline were identified as conjugated amino acids of siderophore extract.
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