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Articles by K.R. Ravikumar
Total Records ( 3 ) for K.R. Ravikumar
  G. Sibi , Saurabh Awasthi , K. Dhananjaya , H. Mallesha and K.R. Ravikumar
  Development of effective means with low risk to human health and environment is needed to control postharvest pathogens as fruits are vulnerable to attacks of various microorganisms upon harvest due to high moisture and nutrient content. Leaves and flowers of Plumeria alba, P. acutifolia and P. rubra were extracted with different solvents to compare the profile of phytochemicals. C. sinensis (sweet orange) was selected to determine the postharvest fungal pathogens of its fruits. Various methods were followed to isolate the fungal pathogens from oranges and investigated their control by using Plumeria species extracts. Phytochemical analysis has revealed the presence of alkaloids as the major phytoconstituent irrespective of plant species and parts used followed by flavonoids and glycosides. Terpenoids, tannins, phenols were the other major phytochemicals found in the extracts. Six fungi were isolated from sweet oranges with Penicillium digitatum as the predominant one followed by Aspergillus sp. and Rhizopus arrhizus. Antifungal assay revealed the potential fungistatic activity of petroleum ether leaf and flower extracts whereas chloroform, methanol and aqueous extracts were completely failed to control the growth of fungal isolates. Flower extracts of P. rubra had the best antifungal activity against all the isolates but with no activity of leaf extracts. However, a significant fungistatic activity was observed with both leaf and flower extracts of P. acutifolia and P. alba. The extracts exhibited significant activity against the blue green mold, P. digitatum. This study suggests the potential value of using crude flower and leaf extracts of Plumeria species to combat postharvest fungal pathogens of sweet oranges thereby extending their shelf life.
  G. Sibi , Rashmi Wadhavan , Sneha Singh , Abhilasha Shukla , K. Dhananjaya , K.R. Ravikumar and H. Mallesha
  Bioactive compounds from plant latex are potential source of antifungic against post harvest pathogens. Latex from a total of seven plant species was investigated for its phytochemical and antifungal properties. Six fungi namely Aspergillus fumigatus, A. niger, A. terreus, F. solani, P. digitatum and R. arrhizus were isolated from infected fruits and vegetables and tested against various solvent extracts of latex. Analysis of latex extracts with phytochemical tests showed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, steroids, tannins and terpenoids. Antifungal assay revealed the potential inhibitory activity of petroleum ether extracts against the postharvest fungal isolates. Various degree of sensitivity was observed irrespective of plant species studied with A. terreus and P. digitatum as the most susceptible ones. F. solani and A. fumigatus were moderately sensitive to the latex extracts tested. Among the plants, latex of Thevetia peruviana (75.2%) and Artocarpus heterophyllus (64.8%) were having potential antifungal activity against the isolates followed by Manilkara zapota (51.1%). In conclusion, use of plant latex makes interest to control postharvest fungal diseases and is fitting well with the concept of safety for human health and environment.
  G. Sibi , Parul Chatly , Sayak Adhikari and K.R. Ravikumar
  Morinda citrifolia L., also known as noni or Indian mulberry is a small evergreen tree having antimicrobial, antitumor, antidepressant and immune enhancing effects. Various parts of M. citrifolia have been investigated for its phytochemical and antimicrobial properties. In the present study, methanolic extracts of leaf, stem and roots of M. citrifolia has been prepared and analyzed for their phytoconstituents. Qualitative analysis of the extracts revealed the presence of phenols, tannins, saponins, alkaloids, glycosides, flavonoids and steroids at various levels. Further, the extracts were tested against pathogenic bacterial and fungal strains at different concentrations to determine the influence of phytochemicals. The results revealed that root and leaf extracts has significant antimicrobial activities mainly due to phenolics and tannins. Staphylococcus epidermidis and Pseudomonas aeruginosa, major pathogens of nosocomial infections were effectively controlled by the extracts at various concentrations and root extract exhibited significant activity against Penicillium sp. and Fusarium sp. It has been revealed that, the bioactive substances have influenced the antimicrobial properties of M. citrifolia which could be exploited to formulate novel drugs from plant origin against bacterial and fungal infections.
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