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Articles by K.P. Unnikrishnan
Total Records ( 3 ) for K.P. Unnikrishnan
  K.P. Unnikrishnan , Anu Fathima , K.M. Hashim and Indira Balachandran
  The present research reports the results of the phytochemical studies carried out to identify the diagnostic features of the E. alba. A high performance thin layer chromatographic method was standardized to determine the wedelolactone content in whole plant of E. alba. Methanolic extracts of samples from three different sources were used for analysis. The mean assay of wedelolactone was of range 0.481-0.702 mg gG1 of drug powder. Radical scavenging activity of methanolic extract was evaluated by DPPH assay method, superoxide radical scavenging activity in riboflavin/light/NBT system and nitric oxide radical scavenging activity in sodium nitroprusside/Griess reagent system. The assay results indicate that the DPPH, superoxide and nitric oxide scavenging activity were intense (19.25, 39.25 and 58.26 μg mL1, respectively). The phytochemical features identified in the present study can be used as identification markers of this important analgesic agent.
  S. Sudhakar Raja , K.P. Unnikrishnan , Satheesh George , A.B. Remashree , P.S. Udayan , K.V. Tushar and Indira Balachandran
  As part of the gene bank activity of CMPR many collections of Adhatoda zeylanica Medic. were made from varying agroecological regions in South India. These collections could be grouped into four morphotypes based on growth habits and other morphological characters. These four morphotypes were evaluated for their chemical and pharmacognostical characters and significant differences among the morphotypes were noticed. Among quality characters vasicine content, fingerprint profiles, stomatal index, leaf architecture and venation pattern showed significant variation indicating the predominance of additive gene effects. A reverse phase HPLC method for the quantitative determination of vasicine in the morphotypes was developed based on which variation in the vasicine content was observed. Thin layer chromatographic analysis showed variation in the chemical composition of these morphotypes. These studies indicated variability in the chemical composition and pharmacognostic characters among the morphotypes of A. zeylanica. Two of the morphotypes containing significantly higher vasicine indicated the presence of chemical diversity, providing adequate scope for selection of superior chemotypes having high therapeutic value and economic benefit.
  Satheesh George , K.V. Tushar , K.P. Unnikrishnan , K.M. Hashim and Indira Balachandran
  Hemidesmus indicus (L.) R. Br. (Periplocaceae) is being used widely in Ayurvedic medicine. The history of its medicinal importance dates back to ancient times. The present review deals with studies undertaken in various aspects of this plant in the areas of morphology, anatomy, pharmacology, chemistry and ethnobotany along with medicinal uses.
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