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Articles by K.P. Kiran Kumar
Total Records ( 3 ) for K.P. Kiran Kumar
  K.P. Kiran Kumar , A.K. Sinha , S.K. Gupta , K.N. Madhusudhan and B.C. Prasad
  Pebrine disease in tasar silkworm is considered to be most serious because of its chronic pathogenicity. Prophylactic method of mother moth examination proves to be the foolproof technique for pebrine identification. So, the study was conducted to investigate pebrine spores detection competency and evaluation of their quality in exhisting mother moth examinations. Four existing mother moth examination methods namely Prick and See, Conventional, Fuziwara and Delayed Mother Moth Examination (DMME) by Fuziwara were selected for the study. The maximum pebrine and hatching percentages were noticed in 4 days DMME with 23.2 and 93.1%, respectively. While, minimum values were observed in prick and see with 4.3% and 84.3, respectively. No significant variations in Cocoon weight, Shell weight and S.R% was observed in cocoons obtained from the different mother moth testing methods. Least percentage of effective rate of rearing (ERR) was observed in Prick and See method (58.75%) followed by Conventional method (61.25%) and Fuziwara (63.15%). High percentage of ERR was observed in DMME which ranged from 64.53% (1st day after egg laying) to 67.28% (4th day after egg laying). Least percentage of improvement of ERR% over Prick and See was observed in Conventional (2.50%) followed by Fuziwara (4.40%). High percentage of ERR over Prick and See was observed in DMME which ranged from 5.78% (1st day) to 8.53% (4th day). Consequently, among the tested mother moth examinations, DMME by Fuziwara after delaying four days from the egg laying has given best results. This method can be implemented in the research institutions for the maintenance of germplasm and breeders stock.
  K.P. Kiran Kumar , G.P. Singh , A.K. Sinha , K.N. Madhusudhan and B.C. Prasad
  Among the diseases of tasar silkworm, virosis caused by Cytoplasmic Polyhedrosis Virus (CPV) is highly contagious and more prevalent. Thirteen antiviral plants were used to test their efficacy against Antherae mylitta cytoplasmic polyhedrosis virus (AmCPV) in tasar silkworm. The aqueous extracts of these plants in different concentrations were used for containment of virosis in virus-infected silkworm and mortality was recorded. The influence of best three phytoextracts which have shown good results in suppressing virosis were subjected for the study of cellular and biochemical changes. Two percent aqueous extracts of Aloe barbedensis (AKP 3), P. corylifolia (AKP13) and Bougainvillea spectabilis (AKP 9) were found more effective in suppressing the virosis and reduced the mortality due to virus infection of 66.17, 64.47 and 57.19%, respectively. The total hemocyte count increased up to 6th day of post inoculation in phytoextract treated batches while in the inoculated control the increase was within 3 days indicating the positive hemocyte mediated response in silkworm treated with phytoextract. The hemolymph protein in Aloe barbedensis treated silkworm (35.27 mg mL-1) was significantly higher than inoculated control (20.25 mg mL-1). The gradual increase of total hemolymph proteins from 1st day (16.31 mg mL-1) to 8th day (33.73 mg mL-1) was observed in healthy control where as in inoculated control increasing trend was observed from day 1 (16.26 mg mL-1) to day 3 (24.22 mg mL-1) there after decreasing trend was observed and finally reached to 20.25 mg mL-1 (8th day). The plant extract of Aloe barbedensis (AKP 3) is more effective in suppressing virosis based on the results of mortality reduction against virosis cellular and biochemical changes.
  K.P. Kiran Kumar , A.K. Sinha , G.P. Singh and K.N. Madhusudhan
  Penicillium citrinum causes white muscardine in tasar silkworm and significantly damage cocoon production at farmer’s field. Four systemic fungicides Bavistin, a carbandazim fungicide (AK 1), Bayleton, a triazole compound (AK 2), Dithane M-45, a mancozeb fungicide (AK 3) and Thiram, a dimethyl dithiocarbamate (AK 4) were tested for efficacy to control white muscardine in, Antheraea mylitta D. One and two percent of AK 1 and AK 3, 0.15 to 2% of AK 2 and 2% of AK 4 in aqueous solution were found to be effective in in vitro condition for the control of muscardine. These fungicides on feeding through the T. arjuna leaves continuously for two days in 5th instar larvae inoculated topically with conidia of Penicillium citrinum (4x106 conidia mL-1) resulted in reduction in mortality due to muscardine by 49-94% as against 100% mortality in inoculated control (control 6). AK 1 reduced the mortality by 93 and 94% in the final instar silkworm at 1 and 2%, respectively. AK 2 at 0.05 and 0.1% concentration reduced the mortality by 85 and 87%, respectively. In case of AK 3 and AK 4 at 1% concentration reduced the mortality by 88 and 69%, whereas at 2% reduced mortality by 90 and 75%, respectively. No mortality was noticed in the controls 1 to 4 which indicated the non toxicity at the particular concentrations. Bavistin (AK 1) and Dithane M-45 (AKP 3) at 1 and 2% concentrations were more effective among tested systemic fungicides in suppressing muscardine in tasar silkworm.
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