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Articles by K.N. Bhilegaonkar
Total Records ( 3 ) for K.N. Bhilegaonkar
  P. Selvan , S.K. Mendiratta , K. Porteen and K.N. Bhilegaonkar
  A study was carried out to determine the influence of different concentration and contact time combinations of Trisodium Phosphate (TSP) solutions on microbial, sensory and physico-chemical characteristics of buffalo offals viz., head meat, heart, liver and rumen. The following concentration and contact time combinations were used: 6% TSP for 15 min, 8% TSP for 10 min and 10% TSP for 5 min. A total of 80 buffalo offal samples (20 numbers of each kind) were collected from a buffalo offal market and subjected to immersion treatments. Water washed offal pieces were used as controls. Sensory evaluations were conducted using a sensory panel comprising postgraduate students and scientists of Livestock Products Technology division, Indian Veterinary Research Institute (India). The data obtained were subjected to statistical analysis using the Analysis of Variance (ANOVA). Mean log10 reductions (CFU g-1) achieved, based on the different treatments and kinds of buffalo offal were between 0.23 and 1.16 for total viable counts; 0.2 and 1.11 for coliforms counts and 0.17 and 0.95 for staphylococcal counts. Immersion in 10% TSP solution for 5 min gave the best overall reduction effect. Sensory evaluations recorded minimal effects of treatments on buffalo offals. These findings show that immersion in 10% TSP solution for 5 min is suitable for decontamination of buffalo offals.
  P. Selvan , S.K. Mendiratta , K. Porteen and K.N. Bhilegaonkar
  A study was carried out to determine the influence of different concentration and contact time combinations of sodium hypochlorite (SHC) solutions on microbial, sensory and physico-chemical characteristics of buffalo offals viz., head meat, heart, liver and rumen. The following concentration and contact time combinations were used: 0.005% SHC for 15 sec, 0.01% SHC for 10 sec and 0.015% SHC for 5 sec. A total of 80 buffalo offal samples (20 numbers of each kind) were collected from a buffalo offal market and subjected to immersion treatments. Water washed offal pieces were used as controls. Sensory evaluations were conducted using a sensory panel comprising postgraduate students and scientists of Livestock Products Technology division, Indian Veterinary Research Institute (India). The data obtained were subjected to statistical analysis using the analysis of variance (ANOVA). Mean log10 reductions (CFU g-1) achieved, based on the different treatments and kinds of buffalo offal were between 0.24 and 1.23 for total viable counts; 0.15 and 1.07 for coliforms counts and 0.07 and 1.00 for staphylococcal counts. Immersion in 0.015% SHC solution for 5 sec gave the best overall reduction effect. Sensory evaluations recorded minimal effects of treatments on buffalo offals. These findings show that immersion in 0.015% SHC solution for 5 sec is suitable for decontamination of buffalo offals.
  K. Porteen , R.K. Agarwal and K.N. Bhilegaonkar
  A polymerase chain reaction was standardized for the identification of Aeromonas sp. using primers against 16S rRNA gene and aerolysin gene. The primers used were found to be highly specific for Aeromonas sp. and did not give positive result with other bacteria including other Gram positive and Gram-negative bacteria. The minimum detection level of PCR was found to be 102 and 104 cells mL-1 in case of 16S rRNA and aerolysin gene targeted assay, respectively. Suitability of the enrichment broth (Alkaline peptone water-cephalothin, APW-C) when tested to detect Aeromonas from the spiked samples gave good results on direct usage of the broth for template preparation without any subsequent treatment. The kinetics of the spiking study indicated that a minimum of 24 h enrichment was required for the detection of Aeromonas by cultural and PCR method. Among two PCR assays detection limits achieved by PCR targeting16S rRNA gene were better than aerolysin gene PCR assay. The results were comparable to cultural method. A total of 100 samples comprising of 50 each of chicken and fish samples were screened by cultural and PCR methods for the presence of Aeromonas. Two chicken samples and three fish samples turned out to be positive by both cultural method and PCR targeting 16S rRNA. From this study it was concluded that PCR assay targeting 16S rRNA gene can be used for the rapid detection of Aeromonas from chicken and fish samples after one step enrichment in APW-C.
 
 
 
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