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Articles by K.N. Bhilegaonkar
Total Records ( 2 ) for K.N. Bhilegaonkar
  Porteen, K. , R.K. Agarwal and K.N. Bhilegaonkar
  A set of primers targeting 16S rRNA gene and aerolysin gene was employed to standardize PCR assay for detection of Aeromonas from milk samples. The primers used were found to be highly specific for Aeromonas spp and did not give positive result with other Gram positive and Gram-negative bacteria. The minimum detection level of PCR was found to be 102 cells -1 and 104cells -1 in case of 16S rRNA and aerolysin gene targeted assay, respectively. Suitability of the enrichment broth (Alkaline peptone water-cephalothin, APW-C) when tested to detect Aeromonas from the spiked samples gave good results on direct usage of the broth for template preparation without any subsequent treatment. The kinetics of the spiking study indicated that a minimum of 24 hrs enrichment was required for the detection of Aeromonas by cultural and PCR method. Among two PCR assays detection limits achieved by PCR targeting16S rRNA gene were better than aerolysin gene PCR assay. The results were comparable to cultural method. Examination of 50 milk samples(Pooled samples) revealed two samples to be positive by cultural method and PCR targeting 16S r RNA.Thus the standardized single –step enrichment PCR protocol holds promise as a reliable and rapid method for the detection of Aeromonas from milk samples.
  P. Selvan , S.K. Mendiratta , K. Porteen and K.N. Bhilegaonkar
  A study was carried out to determine the influence of different concentration and contact time combinations of lactic acid solutions on microbial, sensory and physico-chemical characteristics of buffalo offals viz., head meat, heart, liver and rumen. The following concentration and contact time combinations were used: 1% lactic acid for 20 sec, 1.5% lactic acid for 15 sec and 2% lactic acid for 10 sec. A total of 80 buffalo offal samples (20 numbers of each kind) were collected from a buffalo offal market and subjected to immersion treatments. Water washed offal pieces were used as controls. Sensory evaluations were conducted using a sensory panel comprising postgraduate students and scientists of Livestock Products Technology division, Indian Veterinary Research Institute (India). The data obtained were subjected to statistical analysis using the Analysis of Variance (ANOVA). Mean log10 reductions (CFU g 1) achieved, based on the different treatments and kinds of buffalo offal were between 0.22 and 1.05 for total viable counts; 0.22 and 1.19 for coliforms counts and 0.25 and 0.98 for staphylococcal counts. Immersion in 2% lactic acid solution for 10 sec gave the best overall reduction effect. Sensory evaluations recorded minimal effects of treatments on buffalo offals. These findings show that immersion in 2% lactic acid for 10 sec is suitable for decontamination of Buffalo Offals.
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