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Articles by K.M.D. Islam
Total Records ( 3 ) for K.M.D. Islam
  T. Ahamed , K.M. Hossain , M.M. Billah , K.M.D. Islam , M.M. Ahasan and M.E. Islam
  Newcastle disease virus (NDV) is the infectious agent of Newcastle disease in poultry. This virus can grow within different animal cells including primary cell culture and established cell line. In order to adapt NDV on African green monkey kidney (Vero) cell line, five consecutive passages were done. Eagle`s minimum essential medium (EMEM) with supplements was used for both culturing Vero cells and maintaining NDV on Vero cells. During first and second passage, wild NDV didn`t produce any clear evidence of cytopathic effect (CPE), but in third passage changes in the characteristics of cell monolayer were observed. During fourth and fifth passages, clear and consistent CPE were observed within 50 to 60 hours of infection. CPE was characterized by formation of syncytium, giant cell, dendritic-shaped cell and finally plaque. The titer of passage 5 (P5) virus was 10-3.9TCID50, whose purity was tested by serum neutralization test (SNT) and the result was 1.6 x 104 units/ml.
  P. Shrestha , M.M. Ahasan , K.M.D. Islam , M.M. Billah , M.E. Islam , M. Mehedi , S. Mitra and M.R. Islam
  In the present experiment, Enzyme linked immunosorbent assay (ELISA) was applied on a total of 49 samples collected from 4 breeds of chicken (BV-300, Broiler Kasile, LBM and Hisex) at different age (day 1, day 5, day 10 and day 15) to determine the level of maternally derived antibody (MDA) against infectious bursal disease (IBD). All the chickens were the progeny from the parentstock that had the history of vaccination. A total number of 10 broilers were used to determine the level of IBDV specific antibody in vaccinated and in non-vaccinated chickens following infection with field virus suspension. As these chickens attained the age of 14 days, 6 chickens were vaccinated with Gumboro D78 live vaccine while remaining 4 chickens were kept without vaccination. All the chickens were infected with field virus suspension on day 19 and blood samples collected on day 29 were subjected to ELISA. Slight variation in the antibody titer was observed among 4 breeds of chickens. An average antibody titer of 5320.79, 5877.15, 3676.24 and 5581.55 was found in day old BV-300, Broiler Kasile, LBM and Hisex respectively. Day old BV-300 contained high level of MDA (average of 5320.79) and the level gradually declined and persisted up to 15-20 days. Five days old, 10 days old and 15 days old BV-300 contained an average antibody titer of 3848.57, 2615.53 and 580.88, respectively. On day 29, there was a significant level of antibody (1489.50), much above minimum protection level, in vaccinated chicken whereas nil antibody level was observed in non-vaccinated chickens. Therefore, the chicks should be vaccinated at around day 14, at which time the antibody level reaches nearly to minimum protection level. Antibody level must be carefully monitored at proper interval of time in order to make the vaccination program more effective, to keep the chickens disease free, to increase the production and to prevent the economic loss.
  M.M. Ahasan , M.M. Billah , M.M. Hasan , K.M.D. Islam and J.A. Shilpi
  Two genetically different related forms of human immunodefficiency virus (HIV), a human lentivirus belonging to the lentivirus family, called HIV-1 and HIV-2, have isolated from patients with AIDS. HIV can be transmitted through contaminated blood and blood products; from a mother to her offspring during pregnancy, childbirth or breast feeding; or through sexual contact. Sexual transmission remains by far the predominant mode of transmission. Vertical and blood borne transmission of HIV are highly predictable and very efficient modes. Sexual transmission of HIV, however, appears to be considerably less efficient and highly variable. During the early period after primary infection with HIV widespread of dissemination of the virus and a sharp decrease in the number of CD4+T cell count occurs. The use of CD4 cell count is an important indicator of disease severity of AIDS. The patients infected with AIDS can be attacked by various opportunistic infections such as pneumonia, Mycobacterium avium complex, cytomegalovirus, microsporidiosis and tuberculosis. The degree of intensity of these diseases can be indicated by measuring the level of enzyme like alkaline phosphatase, alanin aminotransferase and aspartate aminotransferase, lactate dehydrogenase and creatine phosphokinase.
 
 
 
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