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by
K.M. Nasiruddin |
Total Records (
2 ) for
K.M. Nasiruddin |
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S. Yasmin
,
K.M. Nasiruddin
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R. Begum
and
S.K. Talukder
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The experiment was conducted in the USDA Biotechnology Laboratory, Biotechnology Department, Bangladesh Agricultural University, Mymensingh to observe the effect of NAA and BAP on callus formation and regeneration from leaf and internodal segment explants. Five levels of each of NAA (0, 1.25, 2.5, 5 and 10 mg l-1) and BAP (0, 0.5, 1, 2 and 4 mg l-1) were applied to MS media for callus induction as well as plantlet regeneration. Twenty explants were cultured in each combination. Leaf showed better performance in callus induction and plantlet regeneration. The highest percentage of callus (95%) was induced with 2.5 mg l-1 NAA + 2 mg l-1 BAP and also minimum time (8.13 days) was required for callus induction in the same concentrations. The percentage of regeneration showed the highest value (80%) with 2.5 mg l-1 NAA + 2 mg l-1 BAP of all the combinations. It was also observed that callus derived from leaf produced plantlets in a shortest period of time (23.68 days) compared to that from stem (28.96 days). |
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K.M. Nasiruddin
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R. Begum
and
S. Yasmin
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The present study was conducted at the USDA Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh during July 2000-July 2002 to investigate the effect of different concentrations of 2,4-D on callusing and PLB formation; BAP (0, 2, 4 and 6 mg l-1) and NAA (0, 0.5 and 1.0 mg l-1) on formation of Protocorm Like Bodies (PLBs) and plantlet regeneration from Dendrobium formosum leaf callus. PLB formation, shoot proliferation, root formation, leaf number, shoot length and root length were recorded. The fastest callusing (9.32 days) and the amount (0.73 g explant-1) was the greatest with 1 mg l-1 2,4-D at 20 DAE, also the number of PLB was the highest (10.52 explant-1) at 60 DAE. Time required for PLB formation was the longest (32.72 days) with 0.50 mg l-1 2,4-D. Plantlet height, root length and number of shoots, leaves and roots per plantlet showed significant variation due to BAP and NAA addition. BAP at 2.5 mg l-1 showed the best results in combination with 1 and 2 mg l-1 NAA. |
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