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Articles by K.M. Kamaruddin
Total Records ( 2 ) for K.M. Kamaruddin
  Nazneen Naher Islam , Zinat Farzana , A.M. Masudul Azad Chowdhury , Adnan Mannan , K.M. Kamaruddin , A.M.A.M. Zonaed Siddiki and Inkeyas Uddin
  The disease mastitis caused by different microorganisms can lead to significant yield losses of milk and its quality. The detection of disease at subclinical stage is much more effective to prevent the occurrence of the disease than the detection of the disease at later stages. For determining the prevalence of Staphylococcus sp. causing Subclinical Mastitis (SCM) in dairy cows in Chittagong, a Southern district of Bangladesh, milk samples were collected from 4 different dairy farms under Chittagong City Corporation. After initial screening by California mastitis test to identify milk samples infected with SCM pathogens, Staphylococcus sp. were isolated and identified by culturing CMT-positive milk samples on selective Mannitol Salt Agar (MSA) medium which was followed by biochemical characterization. PCR was employed to detect S. aureus from Staphylococcus isolates using species-specific primers. Culture Sensitivity (CS) test was done to determine the antibiotic sensitivity pattern of Staphylococcus isolates against 8 commercially available antibiotic discs (ampicillin, amoxycillin, cephalexin, ciprofloxacin, erythromycin, gentamycin, doxycycline hydrochloride and oxytetracycline). To develop plasmid profiles, further extraction of plasmids from Staphylococcus isolates were performed. The results of this research showed that the prevalence of SCM was 74.49%. The 26.71% of milk samples were infected with Staphylococcus sp., 11.64% were contaminated with coagulase positive Staphylococcus and 15.07% with coagulase negative Staphylococcus. Molecular study using PCR revealed that the prevalence of S. aureus causing SCM was 5.48%. CS test of Staphylococcus isolates showed that a high percentage (88.89%) of Staphylococcus isolates were resistant to oxytetracycline while most (94.44%) of the Staphylococcus isolates were susceptible to ciprofloxacin. Plasmid profile analysis revealed that 72.22% of Staphylococcus isolates carried 1 or more plasmids, while further analyses indicate that the multi drug resistance properties may or may not be associated with their presence.
  Nazneen Naher Islam , Mahmuda Akter , Zinat Farzana , Abdul Jabber Bin Kader , Inkeyas Uddin , A.M.A.M. Zonaed Siddiki and K.M. Kamaruddin
  Staphylococcus aureus are gram positive cocci that can cause sporadic cases and outbreaks of food borne illness. The aim of the present study was to detect and identify this organism in samples of refrigerated chicken rinse obtained from different super stores in Chittagong city. The prevalence of infection and antimicrobial susceptibility of Staphylococcus aureus were also studied. The PCR was performed to detect these microorganisms in a chicken rinse microbial consortium and the traditional cultural techniques were performed based on bacteriological analytical manual. To compare PCR and bacterial culture methods for detection of S. aureus, 150 chicken rinse samples from different supermarkets in the Chittagong city were collected and tested. Samples were cultured on selective mannitol salt agar media and contamination by Staphylococcus was confirmed by gram staining, catalase test and coagulase test. Overall 95.83% of the samples were found to be infected with S. aureus. About 68.53% samples were coagulase positive Staphylococcus and 31.46% were negative. Bacterial counts of 100000 or more CFU cm-2 were found on 16.67% of the frozen chicken samples (p≤0.01). Simultaneously, total DNA obtained by thermal extraction from samples was subjected to PCR using a set of primers designed for specific regions of Staphylococcus nuc gene and PCR products were analyzed by agarose gel electrophoresis. Culture sensitivity test and antibiogram study was done to determine the antibiotic sensitivity pattern of Staphylococcus isolates against eight commercially available antibiotic discs (Ampicillin, Amoxycillin, Cephalexin, Ciprofloxacin, Erythromycin, Gentamycin, Doxycycline hydrochloride and Oxytetracycline). All of the samples were resistant to two or more than two antibiotics. The samples showed 100% resistant to Ampicillin, more than 80% were resistant to Oxytetracyclin, Doxycycline hydrochloride and Amoxicillin. Ciprofloxacin showed 77.5%, Cephalexin 38.33% and Gentamycin showed the least resistance 13.33%. The results of this study indicate that the PCR can permit a rapid and reliable means of assessing the bacteriological safety of food and should provide an alternative methodology than conventional viable culture method. The PCR may permit sufficient sensitivity and specificity for the direct detection of Staphylococcus in food samples.
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