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Articles by K.M. Nasiruddin
Total Records ( 4 ) for K.M. Nasiruddin
  M.Z. Rahman , K.M. Nasiruddin , M.A. Amin and M.N. Islam
  Meristem culture of banana cv. BARI-I was performed for virus eradication and shoot multiplication where different concentrations of BAP (0, 1, 2, 3, 4, 5 and 6 mg L-1) and combinations of different level of BAP (0, 4 and 5 mg L-1) and NAA (0, 0.5, 1.0, 1.5 and 2.0 mg L-1) were used. Among the different levels of BAP, better response was found with 5.0 mg L-1 BAP in respect of survivability (91.67%), single shoot formation rate (62.50%) and formation of hard meristamatic ball like structure (29.17%). Maximum shoot multiplication (4.52/explant) with highest shoot length (3.62 cm) was achieved on MS medium containing 4.0 mg L-1 BAP+1.5 mg L-1 NAA treatment. The clusters of multiple shoots were also rooted on MS medium supplemented with 2.0 mg L-1 NAA.
  S.K. Talukder , K.M. Nasiruddin , S. Yasmin , L. Hassan and R. Begum
  The experiment was carried out in the Biotechnology Laboratories Departments of Biotechnology and Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh during July-November 2002, to investigate the effect of BAP and NAA on orchid shoot regeneration. In this experiment shoot proliferation, root formation, leaf number, increment of shoot length and days required for shoot proliferation of orchid cultured on MS media containing different concentrations of BAP (0, 1, 2.5 and 5 mg l‾1) and NAA (0, 0.1, 0.5 and 1 mg l‾1) were observed. The results revealed that the use of different concentrations of BAP and NAA had a significant effect. Among the concentrations, the best shoot proliferation (1.90/explant), root formation (1.93/explant) leaf number (4.25/plantlet), increment of shoot length (0.472 cm) and the least time requirement for regeneration for regeneration (8.8 days) was obtained from 2.5 mg l‾1 BAP + 0.5 mg l‾1 NAA. At the same time, the lowest shoot proliferation (0.05/explant) was found in control and 0.1 mg l‾1 NAA, Leaf number (0.4/plantelt) from 5 mg l‾1 BAP, increment of shoot length (0.252 cm) from control and highest days requirement (23.8 days) from 5 mg l‾1 BAP +0.1 mg l‾1 NAA was found.
  K.H.M.N.H. Nazir , M.B. Rahman , K.M. Nasiruddin , F. Akhtar , M.F.R. Khan and M.S. Islam
  The aim of the research was to find out the effective antibiotic(s) against Escherichia coli and to observe the relationship between the plasmids to the antibiotic resistant pattern found by antibiotic sensitivity tests. For these forty water samples were collected from different sources including river, pond, tap and drain for isolation and identification of pure E. coli. The overall recovery rate of E. coli from water samples was 45%. The highest recovery rate was found from drain water (70%). The pure cultures were subjected to observe the antibiotic resistant pattern by commonly used ten antibiotic disks. All the isolates were found resistant to Penicillin G (94.45%) but 50% isolates were resistant to Amoxicillin. The isolates were highly sensitive to other antibiotics as Ciprofloxacin (88.89%), Chloramphenicol (72.22%), Norfloxacillin (88.33%) and Tetracycline (61.11%). The isolates exhibited moderate sensitivity to Ampicillin (44.44%), Gentamicin (77.78%), Streptomycin (33.33%). Only 22.22, 27.78, 27.78, 16.67, 11.11 and 16.67% of the isolates were recorded to show moderate sensitivity to Amoxycillin, Tetracycline, Chloramphenicol, Cephradin, Ciprofloxacin and Norfloxacillin, respectively. Plasmid profile analysis of 18 isolates were done by 0.8% agarose gel electrophoresis. A total of 11 different plasmid bands of different size were observed by careful eye estimation with the comparison to reference marker. The size of the bands range from 2.4 to 40 kb and at best 5 plasmid bands were found. There was no plasmid in only one isolate. There was no relation found between the plasmid band pattern in agarose gel and antibiotic resistance of E. coli.
  F. Khatun , M.B. Meah and K.M. Nasiruddin
  The study was conducted to develop a protocol for anther culture of eggplant. Six eggplant cultivars viz., Dohazari, IPM 31, Laffa S, Ishurdi L, ISD-006 and Jessore L were implanted on MS medium with different hormone supplements viz., 2 mg L-1 NAA + 2 mg L-1 BAP, 1 mg L-1 IAA + 2 mg L-1 BAP and 2 mg L-1 2, 4-D + 2 mg L-1 BAP for callus induction. The highest survivability of anthers (35 %) and the highest percentage of callus induction (30%) were recorded in cv. ISD-006 with 2 mg L-1 NAA + 2 mg L-1 BAP supplemented MS medium. The minimum days (27.25) required for callus initiation in the cultivar ISD-006 on MS medium with hormone supplement 2 mg L-1 2-4,D + 2 mg L-1 BAP. Anther derived calli of eggplant cultivars were transferred to MS medium with various combinations and concentrations of auxin and cytokinin for shoot regeneration but the calli did not produce any shoot. Root formation was observed from the cultured calli. For different parameters in root formation, the cultivar ISD-006 showed the best performance followed by Jessore L. Among the media, MS medium with 2 mg L-1 NAA +2 mg L-1 BAP and MS medium with 2.5 mg L-1 NAA +2 mg L-1 BAP showed better performance with insignificant variation.
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