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Articles by K.M. Hossain
Total Records ( 3 ) for K.M. Hossain
  T. Ahamed , K.M. Hossain , M.M. Billah , K.M.D. Islam , M.M. Ahasan and M.E. Islam
  Newcastle disease virus (NDV) is the infectious agent of Newcastle disease in poultry. This virus can grow within different animal cells including primary cell culture and established cell line. In order to adapt NDV on African green monkey kidney (Vero) cell line, five consecutive passages were done. Eagle`s minimum essential medium (EMEM) with supplements was used for both culturing Vero cells and maintaining NDV on Vero cells. During first and second passage, wild NDV didn`t produce any clear evidence of cytopathic effect (CPE), but in third passage changes in the characteristics of cell monolayer were observed. During fourth and fifth passages, clear and consistent CPE were observed within 50 to 60 hours of infection. CPE was characterized by formation of syncytium, giant cell, dendritic-shaped cell and finally plaque. The titer of passage 5 (P5) virus was 10-3.9TCID50, whose purity was tested by serum neutralization test (SNT) and the result was 1.6 x 104 units/ml.
  M.M. Ahasan , K.M. Hossain and M.R. Islam
  This experiment was conducted to study the adaptation of IBDV on vero cell line. Suspected IBDV isolates were collected from the bursa of dead chicken of a particular flock. For adaptation, the anchorage-dependant monolayer of vero cell was first subcultured to form semi-confluent monolayer in eagle`s minimum essential medium (EMEM) with 5% fetal calf serum. This semi-confluent monolayer was then infected by local field IBDV isolates. The passage 1 (P1) viruses were harvested and used for the next passage. In this way, the viruses were given three serial passages on vero cell line where characteristic cytopathic effects (CPEs) were observed. During the first passage, no CPEs were found. The infectivity of IBDV on vero cells was observed by changes in the characteristics of vero cell monolayer. During the third passage, clear and consistent CPEs were observed. Vero cell monolayer was changed to form rounding cells. The P3 adapted viruses were confirmed to be IBDV by agar gel precipitation test. The P3 IBDV virus became well adapted to vero cell line.
  M. Mehedi , K.M. Hossain , M.J.F.A. Taimur , B.K. Sil and M.R. Islam
  The research work was undertaken to prepare haemagglutination antigen of NDV on vero cell line as an alternative to the traditional chicken embryonated egg. Newcastle disease virus can grow within different animal cell line. Vero cell is an established cell line whose source is African green monkey`s kidney. The anchorage-dependent vero cells were first subcultured in Eagle`s minimum essential medium to form semi-confluent monolayer. This monolayer was then infected by collected passage 3 (P3) adapted NDV and maintained up to passage 7 (P7). The antigen was collected from this adapted NDV. Then the NDV antigen was assayed and tested for its purity by tissue culture infective dose (TCID50) assay and haemagglutination test respectively. The titre of NDV was 104.1 TCID50. HA result showed NDV antigen agglutinate chicken red blood cells up to 1600 dilution, which is moderately higher titre than HA titre found for NDV, propagated in chicken embryos.
 
 
 
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