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Articles by K.J.P. Narayana
Total Records ( 5 ) for K.J.P. Narayana
  K.J.P. Narayana and M. Vijayalakshmi
  A streptomycete strain, Streptomyces albidoflavus was isolated from soil and culture conditions were optimized for maximum production of α-amylase under submerged fermentation. The optimum period for maximum amylase production was found to be 84 h. The suitable pH and temperature for amylase activity were 6.5 at 30°C. The levels of α-amylase activity detected in culture filtrate varied greatly with type of carbon source used. Soluble starch stimulated α-amylase yield followed by trehalose and maltose. Nitrogen sources like yeast extract, tryptone, NaNO3, peptone and soybean meal were found to support the amylase production by the strain. The strain produced maximum amylase when medium contained starch and yeast extract at concentration of 1.5% (wt./vol.) and 0.2% (wt./vol.) respectively.
  K.J.P. Narayana and M. Vijayalakshmi
  Production of protease by Streptomyces albidoflavus isolated from laterite soil was studied under submerged fermentation. The strain started protease production after 24 h of incubation and maximum level of enzyme production was found with 72 h old culture. Attempts were made to optimize the cultural conditions for getting high yields of enzyme. The optimum levels of pH and temperature for enzyme production were 7.0 and 35°C respectively. Among carbon sources, maltose (1%) supported maximum production of protease followed by trehalose, glycerol, starch and glucose. High yield of protease was recorded in the medium supplemented with peptone (0.75%) followed by beef extract, casein, yeast extract, tryptone and NaNO3.
  K.J.P. Narayana , M. Srikanth , M. Vijayalakshmi and N. Lakshmi
  To study the toxic spectrum of Aspergillus niger, phytotoxicity, cytotoxicity as well as antimicrobial activity were taken as the test criteria. Culture filtrates of A. niger exhibited phytotoxicity against onion and tomato by reducing seed germination and root elongation. The culture filtrates were also tested for cytotoxicity using onion root tip bioassay. Cytological aberrations such as enucleate cells, transfer of chromatin material between adjacent cells and binucleate cells were found in treated onion root tips. Culture filtrates of the fungus grown on yeast extract sucrose broth for one month was extracted with different solvents. Concentrated solvent extracts were tested for antimicrobial activity using disc diffusion method. Butanol and ethyl acetate extracts of the fungal culture filtrates exhibited antimicrobial activity.
  K.J.P. Narayana , V.V.K.V. Rao and M. Vijayalakshmi
  Cultural, morphological and physiological characteristics of Lechevalieria flava were studied. The strain exhibited sensitivity to different types of antibiotics. It could tolerate salt concentration upto 4%. The isolate had the ability to produce enzymes such as amylase, chitinase, protease and urease. Growth pattern and antimicrobial properties of the strain were studied in different media. Impact of various carbon and nitrogen sources on the production of bioactive metabolites was also investigated. The secondary metabolite from L. flava was found to be active against bacteria like Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and fungi such as Candida albicans.
  M. Anupama , K.J.P. Narayana and M. Vijayalakshmi
  While screening the actinomycetes for bioactive metabolites, Streptomyces purpeofuscus was isolated from laterite soil. An attempt was made to screen S. purpeofuscus for the production of antimicrobial metabolites. The cell growth as well as antimicrobial metabolite production was studied on different culture media. Secondary metabolites from the strain were active against bacteria like Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus as well as fungi including Aspergillus flavus, A. niger and Penicillium citrinum.
 
 
 
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