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Articles by K.H.M.N.H. Nazir
Total Records ( 3 ) for K.H.M.N.H. Nazir
  M.B. Rahman , M.M. Rahman , M. Rahman , S.M.L. Kabir , K.H.M.N.H. Nazir and M.M. Amin
  Thirty day-old chicks of Cobb-100 breed with the history of vaccination of parent stock against Newcastle disease (ND) were equally divided into two groups such as group A and B. At 7 days of age of birds, group A was vaccinated with experimentally prepared V4HR-ND vaccine via eye drop @ 106.0EID50/bird to determine the efficacy of this vaccine while group B was kept as unvaccinated control. The birds were used for the study during the period from October 2003 to December 2003. Each bird of group A was boostered with same vaccine @ same dose at 24 days of age. Both the groups (A and B) were challenged after two weeks of post-booster vaccination with 0.25ml inoculum containing 105.0EID50 of virulent field isolates of ND virus intramuscularly. The results of challenge experiment revealed that six birds (40%) of group A succumbed within 3 to 4 days of post-challenge, whereas 15 (100%) unvaccinated control birds of group B showed clinical illness and ultimately died within 3 to 5 days. Thus, the experimental V4HR-ND vaccine conferred 60% protection of vaccinated birds against challenge infection. The mean values of Haemagglutination inhibition (HI) antibody titres of birds in group A were found to have significantly (P<0.01) increased at two weeks of post-booster vaccination. These results indicated that experimental V4HR-ND vaccine induced sufficient humoral immune response which gives satisfactory level of protection against ND.
  K.H.M.N.H. Nazir , M.B. Rahman , K.M. Nasiruddin , F. Akhtar , M.F.R. Khan and M.S. Islam
  The aim of the research was to find out the effective antibiotic(s) against Escherichia coli and to observe the relationship between the plasmids to the antibiotic resistant pattern found by antibiotic sensitivity tests. For these forty water samples were collected from different sources including river, pond, tap and drain for isolation and identification of pure E. coli. The overall recovery rate of E. coli from water samples was 45%. The highest recovery rate was found from drain water (70%). The pure cultures were subjected to observe the antibiotic resistant pattern by commonly used ten antibiotic disks. All the isolates were found resistant to Penicillin G (94.45%) but 50% isolates were resistant to Amoxicillin. The isolates were highly sensitive to other antibiotics as Ciprofloxacin (88.89%), Chloramphenicol (72.22%), Norfloxacillin (88.33%) and Tetracycline (61.11%). The isolates exhibited moderate sensitivity to Ampicillin (44.44%), Gentamicin (77.78%), Streptomycin (33.33%). Only 22.22, 27.78, 27.78, 16.67, 11.11 and 16.67% of the isolates were recorded to show moderate sensitivity to Amoxycillin, Tetracycline, Chloramphenicol, Cephradin, Ciprofloxacin and Norfloxacillin, respectively. Plasmid profile analysis of 18 isolates were done by 0.8% agarose gel electrophoresis. A total of 11 different plasmid bands of different size were observed by careful eye estimation with the comparison to reference marker. The size of the bands range from 2.4 to 40 kb and at best 5 plasmid bands were found. There was no plasmid in only one isolate. There was no relation found between the plasmid band pattern in agarose gel and antibiotic resistance of E. coli.
  M.F.R. Khan , M.B. Rahman , M.S.R. Khan , K.H.M.N.H. Nazir and M. Rahman
  Antibiogram study and plasmid profile analyses were conducted to find out the correlation of the recently isolated Salmonella organisms of Bangladesh. Antibiogram study revealed that the isolates were highly sensitive to Ciprofloxacin, Cephalexin and Kanamycin. In the plasmid profile analysis it was observed that out of 4 isolates of Mymensingh district (M1 to M4), 3 isolates showed 3 plasmid bands in each and 1 isolate showed 4 plasmid bands. The estimated size of the plasmid bands of M1, M2, M3 and M4 were (33.5, 24.50, 4.0, 3.0); (33.5, 3.0, 1.0); (33.5, 3.0, 1.0) and (5.0, 4.0, 1.8) kb, respectively. On the other hand among the isolates of Feni district (F1 to F9), 6 isolates (F4, F5, F6, F7, F8 and F9) showed 5 number of plasmid bands in each and the estimated plasmid band size were same (33.5, 5.0, 4.0, 2.0, 1.8). The plasmid bands of other 3 isolates (F1, F2 and F 3) were found to be different and the estimated size were recorded as 4 (33.5, 5.0, 1.6, 1.5); 3 (33.5, 1.6, 1.5) and 1 (0.7), respectively. Among the isolates of Dhaka district (D1 to D11); no plasmid band was found to be detected in 3 isolates (D2, D5 and D6). The number of plasmid bands of other isolates of D1, D3, D4, D7, D8, D9, D10 and D11 were 4 and the estimated size were determined as (33.5, 15.0, 2.0, 1.0); 5 (33.5, 15.0, 3.0, 2.0, 1.0); 5 (33.5, 5.0, 4.0, 1.8, 1.3); 3 (33.5, 3.0, 1.0); 3 (33.5, 3.0, 1.0); 1(33.5); 2 (33.5, 3.0) and 2 (33.5, 4.0) kb, respectively. All these isolates were tested for antibiotic sensitivity against 8 commonly used antibiotics belonging to different groups. From the antibiogram study it was revealed that all the isolates of Mymensingh and Dhaka district were 100% resistant to Cloxacillin and among the isolates of Feni district 100% were resistant to Erythromycin and Cloxacillin. Plasmid profile analysis of the isolated Salmonella organisms revealed that the isolates carrying multiple plasmids which might be the cause of various degree of antibiotic resistance.
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