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Articles by K.G. Kumar
Total Records ( 2 ) for K.G. Kumar
  A.V. Babu , R.S.C. Rao , K.G. Kumar , B.H. Babu and P.V.V. Satyanarayana
  The plant Merremia emarginata (Burm. f.) Hallier f., belongs to Convolvulaceae family. In traditional medicinal system, different parts of M. emarginata have been mentioned to be therapeutically used as deobstruent, diuretic, for cough, headache, neuralgia and rheumatism. In the present study, biological activities of different solvent extracts isolated from M. emarginata were tested. Hexane (IA), ethyl acetate (IB), methanol (IC) and aqueous methanol (25%) (ID) extracts of M. emarginata were examined. Antioxidant properties of the extracts were studied by DPPH (1,1-Diphenyl-2-Picrylhydrozyl) radical scavenging activity method and superoxide radical scavenging activity method. Methanol extract exhibited better antioxidant activity than other extracts with IC50 of 8.59 μg mL-1 in DPPH radical scavenging method. Methanol and hexane extracts exhibited α-amylase inhibitory activity with IC50 of 104.5 and 133.4 μg mL-1, respectively. Ethyl acetate extract showed cytotoxicity with ED50 of 34.29 μg mL-1 in brine shrimp lethality assay. The present study revealed that the extracts IB and IC of M. emarginata were found to be showed promising biological activities. Methanol extract of this plant might be use full for antioxidant and antiobesity activities with minimal toxicity.
  M. Sridevi , K.G. Kumar and K.V. Mallaiah
  The Rhizobium sp. isolated from stem nodules of Sesbania procumbens (Roxb.) W and A was studied for its ability to produce siderophores on Chrome-Azurol S agar medium. The symbiont was able to produce catechol-type of siderophores in culture after 4 h of incubation. Maximum siderophore production was observed after 24 h. Carbon and nitrogen sources greatly influence the siderophore production. Among the carbon and nitrogen sources, mannitol (2%) and glutamine (0.1%) were found to increase the siderophore production. Thin Layer Chromatography (TLC) of the siderophore extract showed the presence of 2, 3-Dihydroxy Benzoic Acid (DHBA) and 3, 5-DHBA. Arginine, glutamine and proline were identified as conjugated amino acids of siderophore extract.
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