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Articles by K.D. Adjata
Total Records ( 4 ) for K.D. Adjata
  K.D. Adjata , E. Muller , M. Peterschmitt , O. Traore and Y.M.D. Gumedzoe
  Problem statement: This study was carried out to demonstrate that the severity of Cassava Mosaic Disease (CMD) in Togo, is not only influenced by synergism between cassava Begomoviruses in presence, but essentially by recombination between the different Begomoviruses infecting cassava. Approach: Foliar samples presenting typical biological features of Begomoviruses infection were collected from cassava and wild infected plants from different regions of Togo and analysed by PCR targeting the Coat Protein (CP). The PCR products obtained from different isolates of two major Begomoviruses species infecting cassava in Togo were then sequenced and compared with the sequenced of the African cassava mosaic Begomoviruses identified to date and available in NCBI GenBank database by phylogenetic analysis. Results: The results indicate that not only the two major Begomoviruses could be in synergistic interaction in infected cassava in Togo as it has been shown between African Cassava Mosaic Virus (ACMV) and East African Cassava Mosaic Virus (EACMV) elsewhere, but could also create recombinants which would be highly interfering in the development of symptom severity in the country. Conclusion/Recommendations: The study confirmed the assumption that the symptom severity in cassava fields in Togo is rather caused by recombination between different Begomoviruses in presence than by synergistic interaction. More investigations should be done to give insight to this founding.
  K.D. Adjata , E. Muller , M. Aziadekey , Y.M.D. Gumedzoe and M. Peterschmitt
  Cassava is infected by numerous Begomoviruses in Africa and India that cause devastating losses to poor farmers. In order to identify viruses responsible for the disease and characterize them, surveys were conducted in all cassava production zones in Togo. The symptom severity of these viral diseases was recorded. Foliar samples from cassava and other infected plants were collected and analysed by PCR. The results obtained reveal that the severity of the symptoms varies from one locality to another, some more severely cassava infected plants than others. The percentage of the cassava plants presenting typical mosaic symptoms varies from 55 to 85% and the percentage of cassava plants severely infected (score 4 or 5) varies from 4.9 to 36%. Molecular analyses by PCR of the viral DNA extracted from the diseased samples using specific primers revealed for the first time that African cassava mosaic virus (ACMV) is not the only Begomovirus responsible of cassava mosaic disease in Togo, but two other Begomoviruses, East African cassava mosaic virus (EACMV) and Indian cassava mosaic virus (ICMV) are present in Togo. Their incidence rates are 73.59, 44.62 and 4.01%, respectively for ACMV, EACMV and ICMV. Mixed infections were also identified at the rates of 39.45% in the case of (ACMV + EACMV), 1.72% for ACMV + ICMV and 1.29% when the three viruses were combined (ACMV + EACMV + ICMV).
  K.D. Adjata , E. Muller , M. Peterschmitt , M. Aziadekey and Y.M.D. Gumedzoe
  K.D. Adjata , T. Tchacondo , K. Tchansi , E. Banla and Y.M.D. Gumedzoe
  A study was carried out to determine the effect of planting date on Cassava Mosaic Disease (CMD) transmission by white flies (Bemisia tabaci) and its development in a population of cassava clones selected at Lomé Agricultural Experimental Station (LAES) of the High School of Agriculture, University of Lomé in order to contribute in the search of a strategy of effective control of CMD in Togo. To achieve this goal, cassava clones resulting from seeds of the third generation (F3) were planted at different dates on plots P1, P2 and P3 with the interval of 21 days. Three weeks after the establishment of the planted seedlings, data assessment was initiated weekly during twenty weeks. And the measured parameters were: (i) weekly counting of whitefly population on seedlings per plot of 114 m2 and (ii) weekly visual observation to quantify the diseased seedlings according to an assessing note on the severity of the disease ranging from 1 to 5. The results obtained from the visual observations made during this study, revealed that the propagation of the disease on each plot of the evaluated clones is proportional to the size of the population of vectors in presence. Thus, three months after plantation, it was numbered 53% diseased individuals (p<0.05) among the population of cassava clones; at the same time, the population of whiteflies reached an average 1.49±5.23 to 7.83±12.81 individuals by cassava seedlings under the conditions of this work. It was observed that the most significant number of whiteflies was noted on the plots P2 and P3. It was noticed that the plot P3 which was installed six weeks after plot P1, 41% of the seedlings died (p<0.05) due to the infestations of eggs and the larvae of whiteflies.
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