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Articles by K.A. El-Dougdoug
Total Records ( 3 ) for K.A. El-Dougdoug
  M.A. Nasr-Eldin , K.A. El-Dougdoug , B.A. Othman , S.A. Ahmed and S.H. Abdel-Aziz
  Three viroids: Citrus exocortis viroid (CEVd), Hop stunt viroid (HSVd) and Potato spindle tuber viroid (PSTVd) can be detected in naturally infected grapevine by biological indexing and molecular methods. In the present study, direct tissue-printing and dot-blot on the membranes, procedures has been applied on a large scale for an initial screening of HSVd, CEVd and PSTVd in Egypt. The results showed that the tissue print assays allowed clear discrimination between healthy and viroid-infected grapevine plants than dot-blot hybridization, the number of HSVd-infected grapevine plants were 10 plants, PSTVd were 10 plants. The CEVd was detected with high incidence level in grapevine where 12 out of 100 grapevine trees analyzed were infected. The frequency of HSVd, CEVd and PSTVd naturally infected grapevine trees recorded different percentages as in single (8.33, 8.33 and 11.11%), respectively, double (11.11, 11.11 and 19.44%) for CEVd+HSVd, CEVd+PSTVd and HSVd+PSTVd, respectively and mixed infection (19.44%) with different disease symptoms. The pervious results illustrated that, tissue printing hybridization was more reliable than dot-blot hybridization in viroid detection. HSVd was isolated on Cucumis sativus L. cv. Alpha plants which showed specific symptoms severe mosaic, vein clearing, rugosity and yellowing spots. CEVd was isolated on Gynura aurantiaca plants which showed specific symptoms mild mosaic and mottling and Lycopersicon esculantum L. cv. Castle rock reacted with PSTVd producing leaf curl and epinasty.
  K.A. El-Dougdoug , A.A. Rezk , Dawoud A. Rehab and A.R. Sofy
  Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified by polymerase chain reaction (PCR) using Pospiviroid-CCR specific primers. Purified gel RT-PCR product (~199) was cloned into the PCR® II-TOPO® vector then it was sequenced. Partial sequence 199 bp of CSVd-EG is almost identical to that of the prototype 199 bp Canada and USA isolates of CSVd with 96% homology. The sequence of CSVd-EG can be arranged into viroid specific rod like structure. CSVd-EG differ from the prototype isolates Canada and USA at sites occur in regions corresponding to the conserved, variable and right terminal domains which are believed to control viroid pathogencity. Finally, this constitutes the first isolation and identification of CSVd from diseased Chrysanthemum plants in Egypt.
  K.A. El-Dougdoug , A.R. Sofy , G.A. Hameed and R.A. Dawood
  Cucumber mosaic virus (CMV) was isolated from naturally infected Banana, Geranium and Gladiolus and exhibited different external symptoms. The three CMV isolates were transmitted by sap inoculation with 6, 5 and 5 out of 10 inoculated N. glutinosa plants as well as by aphids with 6, 5 and 4 out of 10 inoculated Cucumber plants, respectively. These isolates exhibited different morphological characters of single local lesion on Chenopodium amaranticolor and had different sap stability (TIP, DEP and LIV) in vitro. The differential host reactions were differed among these isolates in incubation period and external symptoms; the amplified cp-gene RNA of confirm the diversity of CMV isolates. It was proved that CMV could involve variation of infection due to one more than CMV strains. It has been declared that the complementary role of biological diagnosis addition, in addition to molecular diagnosis to have a complete and clear image for viral etiology.
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