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Articles by K. Suthindhiran
Total Records ( 3 ) for K. Suthindhiran
  K. Suthindhiran and K. Kannabiran
  Problem statement: Although the diversity of marine Actinobacteria have been studied and biotechnologically exploited throughout the world, the studies on marine actinobacteria in Indian peninsula are largely unexplored. Of 9 maritime states in India, only 4 states have been extensively studied for the diversity of actinobacteria. Further, the studies on bioactive actinomycetes from saline soil are very scanty. In the present study, we had taken an initiative to isolate culturable halophilic actinomycetes and to screen the bioactive potential. Approach: The marine sediment sample was collected at a depth of 400 cm at Marakkanam. The strain was isolated using ISP No. 2 medium supplemented with 25% sea water. The polyphasic taxonomy of the strain was evaluated by phenotypic, chemotaxonomic and phylogenetic analysis. The 16S rRNA was sequenced and phylogenetic relationship with the closest related species were studied. The growth conditions and the medium had been optimized under shake-flask conditions by measuring the dry weight of the mycelium. The isolate was subjected to fermentation and the crude extract was screened for cytotoxic, hemolytic and antimicrobial activity. The cytotoxicity was evaluated on HeLa cells by MTT assay, hemolytic assay on mouse erythrocytes and the antimicrobial activity was determined by agar diffusion assay. Results: Based on polyphasic taxonomy the species was identified as Saccharopolyspora salina and belongs to the genus Saccharopolyspora. The 16S rRNA sequence of the isolate showed 100% similarity with Saccharopolyspora salina. Based on the phylogenetic and phenotypic evaluation the isolate was designated as Saccharopolyspora salina VITSDK4. The growth was maximal in the designed production medium with the incubation temperature of 28°C and pH of 7.4. It is a moderately halophilic species requires 9% NaCl concentration for optimal growth. The cytotoxicity on HeLa cells showed the IC50 value of 26.2 μg mL-1 by MTT assay. The hemolytic activity on mouse erythrocytes showed the EC50 value of 266 μg mL-1. The crude extract also exhibited significant antagonistic activity against fungal pathogens Aspergillus niger, Aspergillus fumigatus, Candida albicans and the Gram negative bacteria Escherichia coli and Klebsiella pneumoniae. Only moderate activity was seen against Gram positive bacteria Staphylococcus aureus and Bacillus subtilis. Conclusion/Recommendations: Based on the results of our investigation, the isolated strain was identified as Saccharopolyspora salina VITSDK4, which was moderately halophilic, produces an extracellular bioactive metabolite, which inhibits the proliferation of HeLa cells as well as antagonistic to fungal and bacterial pathogens. Further studies on purification and characterization of the pure compound from the strain were ongoing. The results of this study suggested that the marine actinomycetes from the unexplored Indian coast could provide lead compounds of therapeutic value.
  K. Suthindhiran and K. Kannabiran
  In the systematic screening programme for cytotoxic compound from marine actinomycetes, the compound furan-2-yl acetate (F2A) from Streptomyces VITSDK1 spp. The structure of the compound was unequivocally determined by spectral studies. It was previously found that F2A has potential antiviral activity against fish nodavirus. In the present study, the cytotoxicity of F2A was studied using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay which showed the IC50 values were less than 15 μg mL-1 against various tumor cell lines, whereas it was >25 μg mL-1 against non-tumor cell lines. F2A inhibited the cell proliferation in a dose-and time-dependent manner. Furthermore, the cytotoxic mechanism was determined in HeLa cells. The morphological analysis, Hoechst staining and DNA fragmentation studies revealed the apoptosis mediated cell death. The cytosolic protein analysis of F2A treated HeLa cells by immunoblotting showed the mitochondrial cytochrome c release, increased expression of caspase 3 and caspase 9 with PARP cleavage. There was no change in the caspase-8 levels. The Bcl-2 was found to be down regulated and Bax was up regulated in the F2A treated cells. Further, the apoptosis induction and cell death was found to be mediated by Reactive Oxygen Species (ROS) and lipid peroxidation. A molecular docking study of F2A with 28 selected cancer drug target enzymes provides some insight on mode of activity of the compound. The findings showed that the F2A exhibits selective cytotoxicity towards tumor cells at a lower concentration via apoptosis.
  S. Banerjee , A. Das , P. Chakraborty , K. Suthindhiran and M.A. Jayasri
  Araucaria cookie is an ornamental plant, which are evergreen conifer found in India and in many other European countries. Similarly Brassaia actinophylla is also an ornamental plant with its native from Java, Australia and in U.S. Though these plants are used for various purposes, the medicinal properties of the plants were not investigated. In our study, the two ornamental plants were chosen for screening both antioxidant and antimicrobial activity. The Leaves of the plants were used for preparing crude extract and was prepared by Soxhlet extraction method. For the extraction of the leave extracts, different solvents viz., methanol, chloroform and petroleum ether were used based on our preliminary data. The obtained extracts were condensed and stored. For the antioxidant and antimicrobial activity, the extractions were prepared into various concentrations. For the antioxidant activity DPPH was used as scavenger of the free radicals which showed the inhibition of percentage for Araucaria cookie was 63% and the inhibition percentage for Brassaia actinophylla 41%. For the antimicrobial activity the extracts were checked against two bacterial and two fungal pathogens. The phytochemical analysis assists in the study of the antioxidant and antimicrobial activity as to the probable compounds responsible for the activity. The result thus obtained provides a report of Brassaia actinophylla as a possible source of antioxidants and also the use of both extracts as a probable antimicrobial agent.
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