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Articles by K. Nemoto
Total Records ( 3 ) for K. Nemoto
  M. Asaduzzaman , M.A. Bari , M. Rahman , M. Minami , K. Matsushima and K. Nemoto
  Agrobacterium mediated transformation has already been reported in rice but under the present investigation efforts has been made to establish the transformation protocol in Bangladeshi rice variety BRRI dhan-30. High percentage of callus induction at 97.3% was obtained when seeds of rice (Oryza sativa L.) cv. BRRI dhan-30 were cultured on modified N6 medium supplemented with 3% sucrose, 2.0 mg L-1 2, 4-D and 0.8% agar under dark condition. Maximum  88.28%  calli  induced  shoots  and  were  obtained from BRRI dhan-30 in the suitable regeneration medium was MS medium supplemented with 3% (w/v) sucrose, 2.0 mg L-1 BAP+0.05 mg L-1 NAA+3% sorbitol and 0.8% agar. Agrobacterium mediated gene transfer protocol for rice variety BRRI dhan-30 were performed using Agrobacterium tumefaciens strain LBA4404, which harbored the plasmid pCL3 containing genes for β-glucuronidase (GUS) and kanamycin resistance. It was found that kanamycin concentration up 50 μg mL-1 were effective for selection of transformants. Putatives transformants as  indicated  by  kanamycin  test  were  subjected  to GUS assay. GUS activities were found in rice calli after co-cultivation.
  M. Asaduzzaman , M. Minami , K. Matsushima and K. Nemoto
  An interspecific hybrid of the Fagopyrum species F. tataricum cv. CT-1 (2x and 4x) and F. esculentum cv. Botansoba (2x) and GreatRuby (4x) was obtained through in vitro ovule culture. Seven to ten days old ovules were excised and cultured on 1/2 MS and MS media and rescued ovules were subjected to different combinations and concentrations of BA, NAA, IAA and zeatin added individually and in various combinations along with sucrose. Effects of the hormones were used to evaluate their potentialities for direct regenerations, callus induction and callus regeneration through ovule culture in interspecific cross between tartary and common buckwheat. Interspecific hybridization in Fagopyrum used ovule culture two methods development in this study. The result showed that no single medium was adequate to ensure complete development of the fertilized ovules to plantlets, thus necessitating a sequential three step transfer to different media. RAPD-PCR analysis confirmed hybridity of the regenerated plant.
  M. Asaduzzaman , M. Minami , K. Matsushima and K. Nemoto
  Cultivated tartary buckwheat (Fagopyrum tataricum) was successfully hybridized with cultivated common buckwheat (Fagopyrum esculentum). Both diploid (2n = 16) and tetraploid (2n = 32) and hybrids were produced from interspecific crosses using ovule rescue method. The produced hybrid was confirmed using plant morphological characters, cytological characters and DNA analysis. The morphological characteristics of the hybrids and parent species are described along with estimation of genetic variation of the hybrids. Self-compatibility together with homomorphism has been introduced from F. tataricum to F. esculentum. Inheritance of flower color in the hybrids was found to be intermediate, whereas pin-style flowers and round shape seeds were found to be dominant over homomorphism and non-winged seeds. There were no obvious differences between the two species in efficiency of pollination. The meiotic observations were accorded with high seed fertility of F1 hybrids and produced normal seeds. These observations indicated that the genetic resemblance and chromosome affinity in the hybrids and parental chromosome supported the fertility of the hybrids.
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