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Articles by K. Nahar
Total Records ( 3 ) for K. Nahar
  Mohammad Masud Rana Mufti , Mst. Parvin Mostari , Gautam Kumar Deb , K. Nahar and Khan Shahidul Huque
  Problem statement: Red Chittagong Cattle (RCC) is a potential genetic resource in Bangladesh having dairy potentialities, high resistance to disease and survive harse environmental condition while require low input supply. This resource is now disappearing due to indiscriminate crossing with other local and exotic cattle. To conserve this resource, find out the genetic diversity of RCC is prime concern. Approach: To determine the genetic variation of Red Chittagong Cattle and to find out the genetic present status for their future improvement and conservation program. The experiment was conducted in the Molecular Genetics Laboratory under Animal Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 100 RCC blood samples and the extracted DNA was observed by gel electrophoresis. Randomly 16 primers were tested among them three primers were matched and found polymorphic. Random Amplification of Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out using DNA samples of RCC. Only unambiguous, reproducible and scorable polymorphic fragments were taken into consideration for analysis. Data were analyzed by using a computer program POPGENE (Version 1.31). Results: The highest and the lowest level of gene frequency value (0.9590 and 0.0440) were observed in allele 0 and 1 with BMC1222-2 locus and only one monomorphic locus (OPB07-1) was found. The Nei’s gene diversity and Shannon’s information indicated that the highest and the lowest diversity were found in Anwara (0.2925) and Chandonish (0.2147) respectively. The pair-wise mean genetic distance value ranged (below diagonal) from 0.0092-0.0279. The minimum distance was found between Satkania and Chandonish. On the other hand maximum distance was found between RCC BLRI and Potia. The dendrogram segregated the five population of RCC into two clusters: RCC BLRI and Anwara is one cluster whereas Satkania, Chandonish and Potia made another cluster. Conclusion: The genetic diversity of RCC is relatively higher for a prescribed breed and therefore, have an opportunities to improve them using selective breeding like ONBS.
  K. Nahar , S.M. Ullah and N. Islam
  The experiment was conducted in the experimental field of Sher-e- Bangla Agriculture University, Dhaka Bangladesh to study the effect of water stress on fruit quality and osmotic adjustment in five tomato cultivars. The percentage of field capacity levels were 40-50, 53-67, 69-85 and 82-100%. The plants had a tendency to adjust against drop in potential in soil by producing organic solutes such as glucose, fructose, sucrose and proline. The quality of fruits was improved as a result of the synthesis of ascorbic acid, citric acid and malic acid. No physical damage due to stress was observed in fruits, which were over 90% red.
  M. Haque , M. Obayed Ullah and K. Nahar
  Crude extracts from different parts (leaf, bark and seed) of Swietenia mahagony (Family: Meliaceae) were screened for their antibacterial activity against 4 Gram positive and 8 Gram negative bacteria. Disc diffusion technique was used for in vitro screening. Among the crude extracts, chloroform and ethyl acetate extracts of leaf and bark showed good activity against all the tested organisms. The chloroform and ethyl acetate extracts of seed exhibited little or positive effect against most of the tested bacteria. The activities were compared to a standard antibiotic-kanamycin. Cytotoxic activity of crude extracts were determined using brine shrimp lethality bioassay and standard vincristine sulphate was used as positive control. The chloroform extract of seed and ethyl acetate extract of bark showed good cytotoxic activities and the LC50 values were found 13.75 and 11.64 μg mL-1, respectively.
 
 
 
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