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Articles by K. Jeeva
Total Records ( 2 ) for K. Jeeva
  M.A. Ravichandran , M. Saminathan , A. Arun Prince Milton , K. Dhama , C. Suresh , K. Jeeva and S.K. Misra
  Cytogenetic studies in domestic animals are gaining more importance because of their genetic implications to breeding programmes. The present study describes the chromosome profile and morphometric characteristics of Garole and Bonpala sheep and comparison of chromosomes between males, females and between breeds. The Karyotype revealed diploid chromosome number of 54 (2n) in both breeds and sexes. The first three pairs of autosomes were bi-armed, submetacentric and remaining 23 pairs were acrocentric. The X-chromosome was acrocentric and largest and Y-chromosome was the smallest, biarmed and metacentric. The morphometric analysis showed significant variation in mean relative length of 13th chromosome pair of Garole and Bonpala males and significant variation in the arm ratio of 2nd chromosome pair of females and variation also noticed in almost all the pairs of chromosomes but not up to the level of significance. The mean relative length of autosomes of Garole and Bonpala male ranged from 1.39±0.05 to 11.45±0.15 and 1.48±0.06 to 11.69±0.25 percentage, respectively. The mean relative length of X-chromosome of the males was 5.66±0.15 and 5.83±0.17, respectively while the Y-chromosome length was 1.20±0.02 and 1.27±0.06, respectively. The mean relative length of autosomes of the females ranged from 1.43±0.06 to 10.80±0.20 and 1.42±0.04 to 11.42±0.36, respectively. The mean relative length of X-chromosome of Garole and Bonpala female was 5.51±0.13 and 5.61±0.15, respectively. The mean arm ratio of first 2 pairs of autosomes of Garole male was higher than Bonpala male while the 3rd pair was higher in Bonpala males. The mean arm ratio of first 3 pairs of autosomes of Garole female was higher than Bonpala female. The present study for the first time compares the cytogenetic profile between Garole and Bonpala sheep breeds.
  M. Suresh Kumar , A.J.A. Ranjit Singh , G. Alagumuthu , K. Jeeva and N. Selvan
  In this study, with the help of Elongation Factor (EF-1α) sequences reported in Apis family analyzed the relationship of the same family in Tamilnadu, South India, India, through nucleotide amplification, sequencing and Phylogenetic tree analysis. The worker honey bee specimens were collected from the colonies in different locations of Tamilnadu, South India. Total Genomic DNA was extracted from worker bees thorax region by using slight modification of high salt extraction protocol and the extracted Genomic DNA were subjected to PCR amplification done by using nuclear protein gene EF-1α primers. Amplified products were analyzed in 1% analytical agarose gel electrophoresis and the molecular weight of the amplified product size of amplicon is approximately ~1100 bp. The amplified product of Apis cerana indica (MSS2) were sequenced and submitted in Genbank (GU935342). Phylogenetic analysis done with other four species sequence data of EF-1α gene collected from Genbank. The phylogenetic tree was constructed by using tree top software program from GeneBee service was started with a set of aligned sequence clustal W. The tree shows clearly that the strain MSS2 is highly cluster with Apis florea and Apis cerana with 100% bootstrap value. Also strain MSS2 had maximum sequence identity (99%) with Apis niqrocincta and Apis cerana than Apis dorsata (96%) and Apis florea (95%). It is assuring that strain MSS2 had a maximum identity 99% and phylogenetically cluster with Apis cerana indica alone. EF-1α analysis of honey bee is an effective tool to classify the honey bee species.
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