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Articles by K. Ito
Total Records ( 3 ) for K. Ito
  C Fujioka , J Horiguchi , M Kiguchi , H Yamamoto , T Kitagawa and K. Ito

OBJECTIVE. The purpose of this study was to investigate the feasibility of prospective ECG-triggered axial 64-MDCT angiography of the aorta and coronary arteries performed at a tube voltage of 100 kV.

SUBJECTS AND METHODS. Thirty patients with a heart rate less than 75 beats/min who were referred for aortic CT angiography were enrolled. The image quality of the ascending aorta, aortic valve, and coronary arteries was evaluated for motion artifacts. Contrast enhancement (mean attenuation) was measured in the ascending aorta, descending aorta, and bifurcation of the aorta. Aortic valve and coronary artery lesions were surveyed.

RESULTS. Acceptable image quality was achieved in 100% (30/30) of cases for the ascending aorta, 97% (29/30) of cases for the aortic valve, and 98% (442/452) of coronary arterial segments. Contrast enhancement was greater than 200 HU and was satisfactory (ascending aorta, 379 ± 80 HU; descending aorta, 354 ± 72 HU; bifurcation, 355 ± 96 HU). Lesions found in the aortic valve were plaque (n = 16) and bicuspid valve (n = 1) and in the coronary arteries were ≥ 50% luminal stenosis (n = 5), plaque (n = 21), myocardial bridge (n = 12), and anomalous origin (n = 1). The effective radiation dose was estimated to be 7.5 ± 1.7 mSv.

CONCLUSION. For patients with a heart rate less than 75 beats/min, prospective ECG-triggered axial CT angiography at a tube voltage of 100 kV has the potential to provide clinically relevant information about the aorta and coronary arteries with low radiation exposure.

  G. O Osoata , T Hanazawa , C Brindicci , M Ito , P. J Barnes , S Kharitonov and K. Ito

Peroxynitrite (PN) formed by the reaction of nitric oxide and superoxide is a powerful oxidant/nitrosant. Nitrative stress is implicated in COPD pathogenesis, but PN has not been detected due to a short half-life (< 1 s) at physiologic condition. Instead, 3-nitrotyrosine has been measured as a footprint of PN release.


PN was measured using oxidation of 2',7'-dichlorofluorescein (DCDHF) in exhaled breath condensate (EBC) collected in high pH and sputum cells. The PN scavenging effect was also evaluated by the same system as PN-induced bovine serum albumin (BSA) nitration.


The mean (± SD) PN levels in EBC of COPD patients (7.9 ± 3.0 nmol/L; n = 10) were significantly higher than those of healthy volunteers (2.0 ± 1.1 nmol/L; p < 0.0001; n = 8) and smokers (2.8 ± 0.9 nmol/L; p = 0.0017; n = 6). There was a good correlation between PN level and disease severity (FEV1) in COPD (p = 0.0016). Fudosteine (FDS), a unique mucolytic antioxidant, showed a stronger scavenging effect of PN than N-acetyl-cysteine on DCDHF oxidation in vitro and in sputum macrophages, and also on PN-induced BSA nitration. FDS (0.1 mmol/L) reduced PN-enhanced interleukin (IL)-1β-induced IL-8 release and restored corticosteroid sensitivity defected by PN more potently than those induced by H2O2 in A549 airway epithelial cells.


This noninvasive PN measurement in EBC may be useful for monitoring airway nitrative stress in COPD. Furthermore, FDS has the potential to inhibit PN-induced events in lung by its scavenging effect.

  T Hamaguchi , T Ito , Y Inoue , T Limpaseni , P Pongsawasdi and K. Ito

Endo-β-N-acetylglucosaminidases are thought to be key enzymes in the catabolism of asparagine-linked oligosaccharides. However, little is known about the enzymes of this type in basidiomycetes. We investigated endo-β-N-acetylglucosaminidases in basidiomycetes using fluorescence-labeled glycoasparagines as substrates. Flammulina velutipes showed high activity and its enzyme was named endo-β-N-acetylglucosaminidase FV (Endo FV). The enzyme purified from the fruiting bodies of F. velutipes was separated into two forms. Endo FV was specific for high mannose and hybrid-type oligosaccharides. The enzyme was remarkably less active against asparagine-linked oligosaccharides attached to glycoproteins. It transferred an asparagine-linked oligosaccharide to Glc, but not to Gal. cDNA of Endo FV was cloned. It was composed of a 996-bp open reading frame encoding 331 amino acid residues. A recombinant Endo FV expressed in Escherichia coli showed enzymatic activity. The Endo FV gene in the genome of F. velutipes had no introns. The gene encoding Endo FV showed little homology with genes of known endo-β-N-acetylglucosaminidases. A chitinase active site motif existed in the deduced primary structure, indicating that Endo FV belongs to glycoside hydrolase family 18. The deduced amino acid sequence of Endo FV had regions conserved in class III chitinases from fungi though it showed little homology with the sequence of any other endo-β-N-acetylglucosaminidases. A folding model of Endo FV indicated it to be homologous with the tertiary structure of Endo H which is quite similar in specificity for asparagine-linked oligosaccharides. This study suggests that Endo FV may become similar to Endo H in substrate specificity as a result of evolutionary convergence.

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