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Articles by K. Gopinath
Total Records ( 2 ) for K. Gopinath
  A. Arumugam and K. Gopinath
  An efficient protocol for in vitro propagation of Terminalia arjuna is described by callus regeneration. Four different explants were used to establish callus to develop shoot and root regeneration method. MS (Murashiege and Skoog), LS (Linsmaier and Skoog) and B5 (Gamborg) basal medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D, 0.1-20.0 mg L-1), with combination of Naphthalene acetic acid (NAA 0.1-20 mg L-1), Indole-3-acetic acid (IAA 0.1-20 mg L-1) and Benzylaminopurin (0.1-20 mg L-1). Ms medium was found to be the most favorable for callus induction compare with LS and B5 media. Maximum number of callus regeneration was obtained on MS medium containing 2,4-D 3.0 mg L-1. The callus culture to develop the shoot and root initiation in MS basal medium substituted with 5 mg L-1 2, 4-D+0.01 mg L-1 Kinetin and 1.0 mg L-1 Gibberellic acid (GA3). The rooted shoot plantlet was transferred in to small plastic cups containing sterile vermiculate, sand and red soil in the ratio of 1:2:2 and were kept in a mist house. The regenerated plantlets were hardened in the greenhouse and successfully transferred in soil with 87% survival rate. This in vitro micro-propagation method with possibility of developing a new protocol was standardized the T. arjuna plant.
  A. Arumugam and K. Gopinath
  An efficient protocol was developed in vitro micropropagation of Gloriosa superba by using corm bud explant. MS basal medium supplemented with different concentration and combination of 2,4-D (2,4-dichlorophenoxy acetic acid), IAA (Indole-3-acetic acid), BAP (6-benzyl aminopurine), GA3 (Gibberellic acid), Zen (Zeatin), NAA (α-naphthaleneacetic acid), AC (Activated Charcoals) and CW (Coconut Water) were used. The 98.30±0.84% of yellowish Callus initiation was observed in MS media with (2, 4-D 1.0 mg L-1, IAA 0.5 mg L-1) after 4 week culture. This corm bud callus transferred to the shoots initiation medium. The maximum number (94.00±2.92) of multiple shoots was obtained on half strength of MS medium with (Kn (Kinetin) 1.0+BAP1.5+20% CW). The shoot lets transferred to the roots initiation medium. The 96.20±2.59% of multiple roots was obtained at the concentration of MS medium with BAP(8.0)+GA3(1.0)+Zen(0.5)+NAA(1.0)+2 g L-1 AC. In other case without addition of activated charcoal in the MS medium, only 92.20±1.92% of root initiation was occurred, 16% of root induction depends on the addition of activated charcoal present in the MS medium. The rooted plantlets were transferred into small plastic nursery tray which was containing vermi compost, sand and red soil in the ratio of 1:2:2 and kept in a mist house. After acclimatization in the mist house for 2-months, the regenerated plantlets were hardened in the greenhouse and successfully transferred into soil which shows 90% survival rate. This new protocol was standardized for easy mass propagation of such an endangered medicinal plant G. superba by using corm bud explants.
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