Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by K. Choi
Total Records ( 5 ) for K. Choi
  P.K. Mandal , A.K. Biswas , K. Choi and U.K. Pal
  Food borne pathogens are a growing concern for human illness and death. There is increasing demands to ensure safe food supply. There is continuous development of methods for the rapid and relible detection of food borne pathogens. Advent of biotechnology has greatly altered food testing methods. Improvements in the field of immunology, molecular biology, automation and computer technology continue to have a positive effect on the development of faster, more sensitive and more convenient methods in food microbiology. Further, development of on-line microbiology, including ATP bioluminescence and cell counting methods, is important for rapid monitoring of cleanliness in HACCP programs. One of the most challenging problems is sample preparation. More research is needed on techniques for separating microorganisms from the food matrix and for concentrating them before detection to ensure food safety, by immunological or nucleic acid-based assays. The possibilities of combining different rapid methods, including immunological and DNA based methods should be further exploited. Further developments in immunoassays and PCR protocols should result in quantitative detection of microorganisms and the simultaneous detection of more than one pathogen or toxin. Lastly, technology continuing to advance at a great pace, the next generation of assays currently being developed potentially has the capability for near real time and online monitoring of multiple pathogens. Modern methods are based on molecular biology techniques like PCR, RFLP, DNA microarray assay, immunological techniques like ELISA, biophysical and biochemical principles with the application of biosensers like bioluminescence sensor, bio-analytical sensors utilizing enzymes, electrical impedometry and flow cytometry. In this review we have tried to summarize the conventional methods and newly developed rapid pathogen detection techniques and the need for newer and rapid methods are discussed.
  H.K. Lim , K. Choi , P.K. Mandal , O. Baatartsogt , C.H. Lee , J.H. Lee and H.B. Kim
  This study was carried out to investigate the differentially expressed genome between S. gallinarum infected and uninfected control in the spleen lymphocytes of Ross broiler chicks using microarray analysis. GeneChip Chicken Genome Array containing 32,773 transcripts corresponding to over 28,000 chicken genes for simultaneous expression was used. The signal intensity of each gene was normalized and expressed in fold change. A large numbers of genes were found with differential expression majority of which are still unknown in chicken genome. Thirty one known genes were found to have differential expression of which, 25 were up-regulated and 5 were down regulated. Majority of the up-regulated genes belong to immune response system viz., IL8, IL1B, IL10, IL18, IL17A, IL15, transferrin, IFNg, TLR2, TNFRSF1b, TNFRSF15 and the down regulated genes were B-FIV, B-LA, SDF1, B-LBI, belonging to MHC-I and II and CD1d. To validate the expression of these genes RT-PCR was done using primers of 12 selected genes′ with total mRNA isolated from spleen lymphocytes which has confirmed the similar pattern of expression of all the genes as in microarray. The findings in this study have lead to the identification of novel genes which may be useful in further studies to understand the patho-physiology of fowl typhoid towards development of diagnostics and therapeutics.
  Go-Eun Hong , P.K. Mandal , Chang-Won Pyun , K. Choi , Soo-Ki Kim , Kyu-Ho Han , M. Fukushima , Ho-Chul Shin and Chi-Ho Lee
  This study was done to investigate the effects of fermented soy pulp on the osteoporosis in ovariectomized rats. Sprague-Dawley female rats were randomly assigned to four groups as Sham Control (SC), Ovariectomized Control (OC), Ovariectomized and Soy Pulp (OSP) fed and Ovariectomized and Fermented Soy Pulp (OFSP) fed. All rats were fed on purified diets, supplemented with non-fermented and fermented soy pulp on basic diet for 7 weeks. It was observed that isoflavone aglycone was very high in soy pulp fermented for 12 h in comparison to non-fermented soy pulp. Body weight of the rats increased significantly (p<0.05) in comparison to other groups. Atrophy of uterus in OFSP group was significantly (p<0.05) prevented in comparison to OC group. The concentration of estradiol in OFSP group was higher than those of OC and OSP groups. The bone density in OFSP group was significantly (p<0.05) higher than those of OC and OSP groups. The histopathology indicated that OFSP group has better retarded the progress of osteoporosis than other groups. The results showed that isoflavone from the fermented soy pulp has prevented the osteoporosis in ovariectomized rats must be due to its estradiol like function. It is expected that the fermented soy pulp might serve as a functional food in osteoporosis of postmenopausal women.
  O. Baatartsogt , K. Choi , P.K. Mandal , H.K. Lim , J.D. Oh and S.H. Chang
  This research was done to study the protein expression profile of synovial cells of knee using proteome analysis during the development of experimental Post-Traumatic Arthritis (PTA) in swine. PTA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in three piglets. Articular cartilage and synovial tissues were obtained after 0, 2, 5 and 8 weeks for histopathologic examination. After sacrificing the piglets at 8 weeks, synovial tissues were collected for 2-dimensional electrophoresis and mass spectrometric analysis. Histopathologic examination at 8 weeks showed overt chronic inflammation indicating the development of ETA. Through proteome analyses more than 1,500 protein spots were identified in which 7 differentially expressed protein spots were observed in ACL-transacted synovial tissue. Five proteins were down-regulated (cytoskeletal β actin, cofilin-1, destrin, Rho GDP dissociation inhibitor α and an unnamed protein product) and two proteins were up-regulated (α-B crystalline and Smooth Muscle Protein (SMP) 22α). These results showed that proteins that are related to cellular organization and signal transduction are down-regulated and those that are related to cell rescue, defense and stress are up-regulated. Therefore, the proteome analysis of synovial tissue provided us new candidate molecules which may be useful to understand the pathogenesis for diagnostic and therapeutic studies of Post Traumatic Arthritis (PTA).
  O. Baatartsogt , K. Choi , P.K. Mandal , Hee-kyong Lim , Guan-Hao Li , Hong-Sik Kong , Dae-Hyun Hahm , Chi-Ho Lee and Jun-Heon Lee
  To get insights into pathological pathways of Post Traumatic Arthritis (PTA) this study was done on gene expression profile of synovial cells of knee using gene chip analysis developing experimental post traumatic arthritis (ETA) in swine. ETA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in 3 piglets. Articular cartilage and synovial tissues were obtained after 0, 2, 5 and 8 weeks for histopathologic examination. Synovial cells collected after sacrificing the piglet at 8 weeks, were used at 5 passage for gene expression profiling using Affimetrix Gene Chip. Histopathologic examination showed overt chronic inflammation indicating the development of ETA. Through genome analyses it was observed that 87 known genes were up-regulated and 76 known genes were down regulated. By analyzing, it was found that many genes with differential expression are related to inflammation, immune response, lipid binding, cell adhesion, growth activity and muscle development. The present study provided an insight into the TA related gene expression pattern. The genome analysis of synovial cells provided us new candidate molecules which may be useful to understand the pathogenesis of Post Traumatic Arthritis (PTA). The established porcine model may serve as in vivo disease model for further research on traumatic arthritis to elucidate molecular pathogenesis.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility