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Articles by K. Sushil
Total Records ( 3 ) for K. Sushil
  W.H. Himratul-Aznita , T.B. Taiyeb Ali , K. Sushil , S.L.A. Zainuddin , A.A. Mahmood and A. Ansary
  The study was undertaken with the aims of identifying strains providing virulent toxin and to observe the histological effect of the toxin in mice. Plaque samples were collected from adult periodontitis patients. P. intermedia were recovered from subgingival periodontal pocket with depths of 5 mm or greater. Ninety P. intermedia isolates were identified based on its bacteriological properties, gram staining and biochemical characteristics. The clinical isolates of P. intermedia were assessed for their potential and ability to produce toxin and form skin lesion in balb/c mice. 108, 1010 and 1012 cells mL 1 of bacterial suspension were used in the study. No lesion was observed in mice injected with 108 cells mL 1 and only three P. intermedia with concentration 1010 cells mL 1 were able to induce localized skin lesion in balb/c mice. However, all isolates causes balb/c mice to develop localized skin lesion when 1012 cells mL 1 was used. Infected mice appeared cachectic and the histological effect of the skin lesion showed that all lesions were localised at the injection site and causes tissue damage with skin necrosis and hair loss.
  S. H. Lim , I. Salmah , W. L. Ooi , K. Sushil , A. M. Sahilah , R. Son , Moushumi Ghosh , Sunita Bansal and Abhijit Ganguli
  One of the uniqueness of Escherichia coli O157:H7 is it inability to ferment sorbitol in 24 h and its negative test with the MUG assay, although this organism carried the UidA gene which encodes for -glucuronidase in its chromosome. Primers were designed based on the sequence of the -glucuronidase gene and were evaluated in a polymerase chain reaction assays as a marker to detect E. coli O157:H7. Of the three pairs of primers tested, which produces the estimated product size of 352, 271 and 353 bp respectively, one primer pairs (UidA2F & UidA2R) was found to be more specific and useful as a marker in combination with a published primer for the H7 gene for the detection of Escherichia coli O157:H7.
  S. H. Lim , I. Salmah , W. L. Ooi , K. Sushil , A. M. Sahilah and R. Son
  One of the uniqueness of Escherichia coli O157:H7 is it inability to ferment sorbitol in 24 h and its negative test with the MUG assay, although this organism carried the UidA gene which encodes for -glucuronidase in its chromosome. Primers were designed based on the sequence of the -glucuronidase gene and were evaluated in a polymerase chain reaction assays as a marker to detect E. coli O157:H7. Of the three pairs of primers tested, which produces the estimated product size of 352, 271 and 353 bp respectively, one primer pairs (UidA2F & UidA2R) was found to be more specific and useful as a marker in combination with a published primer for the H7 gene for the detection of Escherichia coli O157:H7.
 
 
 
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