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Articles by Jutti Levita
Total Records ( 8 ) for Jutti Levita
  Hanny Nugrahani , Arif S.W. Kusuma , Jutti Levita and Rizky Abdulah
  Background and Objective: Selenium has been reported to improve the anticancer activity of broccoli sprouts and florets. However, the anticancer properties of the combination of selenium and sulforaphane, the active anticancer compound in Broccoli have not been well investigated. This study was conducted to investigate the role of the combination of selenium and sulforaphane in inhibiting the proliferation of MCF-7 cells. Materials and Methods: Methylseleninic acid was used in this study as it can directly convert to monomethylated selenium for exhibiting its anticancer activity. The anti-proliferative properties of both sulforaphane and methylseleninic acid were analyzed separately and in combination with MCF-7 cells using WST assays. The combinations of sulforaphane and methylseleninic acid in ratios of 1:2 and 1:3 were analyzed using the Chou-Talalay method. Results: The 24 h IC50 values of sulforaphane and methylseleninic acid alone against MCF-7 cells were 24.55 and 182.35 μM, respectively. Furthermore, the results of the Chou-Talalay equation suggested that the combinations of sulforaphane and methylseleninic acid in ratios of 1:2 and 1:3 showed combination index values <1 at all tested concentrations. Conclusion: This study suggested that the combinations of sulforaphane and methylseleninic acid exert synergistic antiproliferative properties against the MCF-7 breast cancer cell line.
  Jutti Levita , As`ari Nawawi , Abdul Mutalib and Slamet Ibrahim
  This study focuses on the anti-inflammatory activity of andrographolide, a diterpenoid compound from Andrographis paniculata, that have been observed in animals and in vitro in different cells of human and animals. Studies included activities of andrographolide and the features of the target, especially limited to transcription factors NF-kappaB. The active site of NF-kappaB, called DNA binding region is found to have mainly positive potential. In order to have an electrostatic complementarity specific inhibitor should have negative potential. Andrographolide has three hydrogen bond donors (H atoms in hydroxyl groups attached to C-3, C-19 and C-14), five hydrogen bond acceptors (O atoms in hydroxyl groups attached to C-3, C-19 and C-14, carbonyl and lactone) and log P-value 2.9. It fulfills Lipinski’s rules of five criteria of drug properties. This compound has different mechanisms of anti-inflammatory activity. It can inhibit the activation of NF-kappaB, suppresses inducible nitric oxide synthase (iNOS) expression. It also prevents oxygen radical production by human neutrophils and inhibits COX-2 expression in human fibroblast cells. This compound also exerts anticancer and antitumour activities, hepatoprotective against various inducers, immunomodulator, antioxidant, antidiabetic, antimicrobe and antivirus activities. Synthetic analogues of the compound which have been created and analyzed also showed similar activities.
  Jutti Levita , As `ari Nawawi , Abdul Mutholib and Slamet Ibrahim
  The aim of this study was to identify and analyze the interaction of andrographolide with COX-2, followed by in vitro study of the effect of this compound on COX-2 expression in human fibroblast cells. The molecular modeling study was performed by docking andrographolide to COX-2 enzyme at the site where SC-558, a selective inhibitor of this enzyme, was co-crystallized and compared its interaction to the enzyme with SC-558’s. The inhibition of COX-2 expression was determined by measuring PGE2 production in human fibroblast cells stimulated with LPS with and without andrographolide preincubations. Andrographolide interacted with Arg513 and His90 in the cyclooxygenase site of COX-2 and inhibited PGE2 production in human fibroblast cells (IC50 = 4 μM). These data confirm that andrographolide’s anti-inflammatory activity occurs via inhibition of COX-2 expression.
  Jutti Levita , Laura K. Wijaya , Sharren Celcilia and Mutakin Mutakin
  Cyclooxygenase (COX) or Prostaglandin H2 Synthase (PGHS) is the enzyme that catalyzes the first two steps in the biosynthesis of prostaglandins from the substrate, arachidonic acid. The NSAIDs work by inhibiting both COX isoforms, thus the conversion of arachidonic acid into prostaglandin is disturbed. In our country, kaempferia rhizome (Kaempferia galanga) and roselle calyx (Hibiscus sabdariffa) are used to reduce inflammation by consuming herb tea of these plants. This work studied the inhibitory activity of kaempferia rhizome and roselle calyx on the rate of prostaglandin formation by measuring the absorbance of TMPD (tetramethyl-p-phenyldiamine) oxidized by the extracts, against time. The plants were collected from Manoko plantation in West Java. Phytochemical screening showed that the rhizome contains quinones and terpenes, while the calyx contains polyphenol, flavonoid, quinone and saponin. 100 g of dried rhizome and calyx were separately boiled in 1 L of distilled water for 15 min at 90°C, freeze-dried and dissolved in ethanol 96%. Both Kaempferia galanga and Hibiscus sabdariffa showed inhibition on PGH2 formations. The rate of PGH2 formations on COX-1 was lower than on COX-2. These plants could be further developed for anti-inflammatory drug.
  Tanti Juwita , Irma Melyani Puspitasari and Jutti Levita
  In order to propose a prospective candidate for novel complementary phytopharmaceuticals, one of Zingiberaceae family plant, Etlingeraelatior or torch ginger, was being evaluated. The aim of this review was to provide a comprehensive literature research focused on the botanical aspects, nutritional quality, phytoconstituents and pharmacological activities of E. elatior. Researches on this particular plant were conducted in Malaysia (55.5%), Indonesia (33.3%), Thailand (8.3%) and Singapore (2.7%). This review article has revealed that the most prominent pharmacological activities were anti-microbial, anti-oxidant and anti-tumor activities in consistent with the dominated levels of flavonoids, terpenoids and phenols. However, extended and integrated research should be converged towards intensive investigations concerning to isolated phytoconstituents and its bioactivities, pharmacokinetics, bioavailability, molecular mechanism of its specific pharmacological activities, safety and efficacy studies for further development.
  Aziiz Mardanarian Rosdianto , Irma Melyani Puspitasari , Ronny Lesmana and Jutti Levita
  Background and Objective: Flavonols in plants are catalyzed by flavonol synthase (FLS) enzyme. FLS was reported expressed in flowers and fruits, i.e., Dianthus caryophyllus L. (Caryophyllaceae), Petunia hybrida Hort. (Solanaceae), Arabidopsis thaliana L. (Brassicaceae), Citrus unshiu Marc. (Rutaceae). However, none reported about FLS in medicinal plants, particularly those which possess anti-inflammatory activity. This study was aimed to extract and identify FLS in the rhizome of Boesenbergia rotunda (Zingiberaceae) and to determine quercetin in the ethanol extract of the rhizome. Materials and Methods: The protein extraction of the rhizome was carried out by employing Laing and Christeller’s (2004) and Wang’s (2014) methods. The extracted-proteins were separated by using SDS-PAGE, followed by the measurement of FLS intensity by using Gel Analyzer. The FLS-1 of recombinant A. thaliana was employed as the standard. The determination of quercetin in the rhizome was carried out using LC-MS. Results: The FLS occurred as a thick band at 38 kDa with intensity 116-158. The LC chromatogram of the extract indicated a small peak at 7.94 min similar to that of quercetin standard. The MS spectra at 7.94 min indicated that quercetin is present in the B. rotunda rhizome (m/z = 303.0549). The concentration of quercetin in the extract is 0.022% w/v. Conclusion: The FLS, an enzyme which plays an important role in producing quercetin, was detected in B. rotunda rhizome planted in Indonesia. As a consequence, quercetin in a small amount, was also quantified in the rhizome of this plant. This report will add a scientific insight of B. rotunda for biological sciences.
  Tanti Juwita , Wenni H.P. Pakpahan , Irma M. Puspitasari , Nyi Mekar Saptarini and Jutti Levita
  Background and Objective: Etlingera elatior (Jack) R.M. Smith rhizome, which has been traditionally used to reduce stomach discomfort, was reported to possess anti-inflammatory activity, however, there is a lack of such a study on the flower. Materials and Methods: To investigate the anti-inflammatory activity of the E. elatior flower extract on gastric ulceration-induced Wistar rats. The Wistar rats were divided into 6 groups. Group 1 was the normal control, group 2 was the negative control (Arabic gum suspension 2%), group 3 was the positive control (quercetin), group 4-6 were treated with E. elatior flower extract dose of 500, 1000 and 2000 mg kg1 of b.wt., respectively. The rats were conditioned to gastric ulceration. The stomach weight, microscopic and macroscopic evaluation of gastric mucosal damage was examined. Subsequently, the nuclear factor-kappaB-p65 (NF-kappaB-p65) expression in the fundus was Western-blotted by employing β-actin and GAPDH as the loading controls. Results: Etlingera elatior flower extract dose of 1000 mg kg1 b.wt., reduces the ulceration index and the infiltration of inflammatory cells. Western blot analysis showed inhibition of NF-kappaB-p65 expression by E. elatior flower extract dose of 1000 mg kg1 of b.wt. Conclusion: Etlingera elatior flower might possess anti-inflammatory activity by downregulating the expression of NF-kappaB-p65 in the fundus of gastric ulceration-induced Wistar rats.
  Jutti Levita , Aulia Alfiana , Melissa Bernadette , Rina Fajri Nuwarda , Sri Adi Sumiwi , Nyi Mekar Saptarini and Suseno Amien
  Background and Objective: Inflammation occurs via several mechanisms, one of which includes the production of Nitric Oxide (NO) catalyzed by inducible nitric oxide synthase (iNOS), which is inhibited selectively by isothioureas. Ageratum conyzoides L. has shown activity in reducing pain and inflammation, although the molecular mechanism had not been undertaken. The objectives of this work were (1) to study the mechanism of anti-inflammatory activity of A. conyzoides through inhibition of iNOS, (2) to correlate the iNOS inhibitory activity of the plant with the total flavonoid content of the plants and (3) to identify the flavonol synthase (FLS), an enzyme that catalyzes the production of quercetin. Materials and Methods: The inhibitory activity against iNOS was assayed by in vitro method. The total flavonoids (calculated as quercetin) of A. conyzoides were determined by fluorometry. The protein extraction of the leaves was carried out by employing Laing and Christeller’s (2004) method, followed with SDS-PAGE. Results: The inhibitory activity (IC50) of ethanol extract and ethyl acetate fraction of A. conyzoides against iNOS was 92.05 and 4.78 μg mL1, respectively. Pearson correlation analysis resulted in 0.548 (ethanol extract) and 0.696 (ethyl acetate fraction). The total flavonoids (calculated as quercetin) contained in the ethanol extract and ethyl acetate fraction of A. conyzoides were 0.71 and 7.65%, respectively. The FLS in A. conyzoides leaves was identified at 31 kDa. Conclusion: A. conyzoides L. is potential in inhibiting iNOS due to quercetin contained in the leaves. This report will add a scientific insight of A. conyzoides for biological sciences.
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