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Articles by Justus M. Onguso
Total Records ( 3 ) for Justus M. Onguso
  A.B.M. Sharif Hossain , F. Mizutani and Justus M. Onguso
  Effects of partial and complete ringing in the trunk on overall nutrient status of peach (Prunus persica Batsch. cv. `Hikawahakuho`) trees were examined by using one-year-old trees. The partial ringing comprised removal of 2 cm length bark and remaining 2 mm strip whereas complete ringing no remaining strip. Organic (sugar and starch) and inorganic nutrient content in leaves, stems and roots were monitored for eight weeks after ringing. The treatment reduced overall reduction in sugar and starch content in all plant parts. Each sugar component also showed similar trends. The N and Ca content in the above ground parts were lower in the ringed plants than control but the reverse was true in the roots. On the other hand, P, K and Mg content in all plant parts were declined by the treatment. Partial ringing prevented the translocation of 13C-photosynthates from the above ground parts to roots although entire inhibition was apparent in the complete ringing. Such reduction in energy sources of the roots seemed to affect overall plant nutrition which leads to small sized trees.
  Justus M. Onguso , Esther M. Kahangi , Douglas W. Ndiritu and Fusao Mizutani
  This study was carried out with the objective of determining the optimum reaction conditions for the polymerase chain reaction (PCR) in Musa germplasm. DNA was extracted from young leaves and purified. Amplification reactions were carried out with different concentrations of taq DNA polymerase, MgCl2 as well as template DNA for the PCR. For the RAPD analysis of Musa germplasm. The ideal concentration of the thermostable polymerases, MgCl2 as well as the template DNA for the PCR was defined. As a result, the optimum concentrations were 3 mM for MgCl2 , 20 ng for template DNA and 0.70u for taq DNA polymerase. These were the concentrations that yielded reproduceable amplification products in different replications with Kenyan Musa germplasm.
  Justus M. Onguso , Fusao Mizutani , A.B.M. Sharif Hossain and Ali R. El-Shereif
  Effects of net and cheesecloth cover on the growth, yield and fruit quality of peach (Prunus persica [L.] Batsch) trees were investigated in summer pruned trees after fruit harvest. Trees were covered with white cheesecloths (giving 30% shade) or nets. The length of regenerated shoots from the branches covered with cheesecloths and nets was one third and half as great as the control, respectively. Covering with cheesecloth reduced flower bud formation. It also led to increased Bix but decreased acidity to a slightly greater degree than nets. Both cheesecloth and net treatments increased ethylene production and ACC content in the shoot tips. Earlier flowering was noted in the following spring in the trees that had been covered with cheesecloths. Such early flower opening, which corresponded to early bud break, seems to be associated with ethylene biosynthesis caused by thigmomorphogenesis.
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