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Articles by Junbo Zhang
Total Records ( 3 ) for Junbo Zhang
  JunBo Zhang , ZhiXin Song , Chu Jinfeng and XueBin Wang
  The [99mTcN(PNP5)(DMCHDTC)]+(DMCHDTC: 2,3-dimethyl cyclohexyl dithiocarbamate, PNP5:bis(dimethoxypropylphosphinoethyl)ethoxyethylamine) complex was synthesized through a ligand-exchange reaction. The two-step procedure involved the initial reaction of 99mTcO4 with succinic dihydrazide (SDH) as a donor of nitride nitrogen atom (N3−) in the presence of stannous chloride dihydrate as reducing agent and propylenediamine tetraacetic acid (PDTA) as complexant, followed by the addition of the PNP5 ligand and the DMCHDTC ligand. The radiochemical purity (RCP) of the product was over 90% as measured by thin layer chromatography (TLC). No decomposition of the complex at room temperature was observed over a period of 6 h. Its partition coefficient indicated that it was a lipophilic complex. The electrophoresis results showed the complex was cationic. The biodistribution results in mice indicated that [99mTcN(PNP5)(DMCHDTC)]+ was significantly retained into the heart. The heart uptake (ID%/g) was 14.47, 12.23 and 8.76 at 5, 30 and 60 min post-injection, respectively. The heart/liver, heart/lung and heart/blood ratios of the complex were 1.24, 3.62 and 23.05 at 60 min post-injection, suggesting it will be a potential myocardial imaging agent.
  Fei Guo , Yuanzhi Wang , Chuangfu Chen , Hui Zhang , Jun Qiao , Yan Ren , Junbo Zhang and Zhiqiang Li
  TFSS is an important virulence factor of Brucella, organized as one operon containing 12 different across the cell wall bacterial proteins among which VirB5 regulates the host phagocytosis of Brucella and the transportation of Brucella in the host cells. This study has constructed cDNA library from Brucella melitensis 16M-infected murine macrophage Raw264.7, identified and confirmed the interaction between Brucella VirB5 and FTH1 of RAW264.7 using yeast two-hybrid and Co-Immunoprecipitation (Co-IP) technologies. Subsequently, the morphological changes and the expression of apoptosis-related genes in Brucella-infected RAW264.7 cells have been investigated with Electron Microscope (EM) and real-time quantitative RT-PCR, respectively. The present study has demonstrated that VirB5 and FTH1 play important roles in intracellular parasitism of Brucella and inhibition of FHT1 expression accelerates the apoptosis of macrophage.
  Fei Guo , Junbo Zhang , Chuangfu Chen , Yuanzhi Wang and Hui Zhang
  Brucellosis is an infectious disease that brings great economic burdens for developing countries. The vaccine S2 which is an attenuated Brucella suis (B. suis) strain has been used on a large scale in China. However, the immunity induced by S2 declined relative to those vaccinated with Rev-1 and S19 vaccines. Moreover, the vaccine S2 cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent B. suis vaccine is needed. In this study, a vjbR mutant of B. suis (B. suisΔvjbR) was constructed overcome these drawbacks. The B. suisΔvjbR strain showed reduced survival capability in RAW264.7 macrophage and mice and induced high protective immunity in mice. In addition, B. suisΔvjbR induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of gamma interferon (INFγ). Further, the vjbR antigen allowed serological differentiation between natural and vaccinated infection in mice. Therefore, B. suisΔvjbR was suggested as a safe and efficacious live vaccine candidate against virulent Brucella suis (B. suis) infection.
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