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Articles by Junaidi
Total Records ( 2 ) for Junaidi
  Ocky Karna Radjasa , Agus Sabdono , Junaidi and Elena Zocchi
  A total of 8 bacterial isolates associated with sponge Haliclona sp. collected from Bandengan water, Jepara, North Java Sea, Indonesia, was successfully screened for antibacterial activity against pathogenic bacteria Vibrio parahaemolyticus, Aeromonas hydrophila and Staphylococcus aureus. Active bacterial isolates were rapidly grouped by using rep-PCR and a dendrogram was constructed. Five isolates were selected based on the constructed dendrogram for subsequent DNA sequencings resulted in the richness of secondary metabolite-producing sponge associated-bacteria having closest similarity to Vibrio parahaemolyticus, Pseudovibrio denitrificans, Pseudoalteromonas sp., alpha proteobacterium and uncultured bacterium clone. The present study highlighted the repetitive-PCR method as a powerful tool for estimating the richness of secondary metabolite-producing parts among sponge colonizers.
  Hafrijal Syandri , Azrita , Junaidi and Ainul Mardiah
  Background and Objective: Maninjau lake is one of the important locations for aquaculture activity for many local people in Indonesia. The objective of the current research was to estimate the Nitrogen (N), Phosphorus (P) and Total Organic Matter (TOM) before and after fish mass mortality in Maninjau lake. Materials and Methods: This research was conducted in February, 2017, four months after mass mortality occurred. Nile tilapia (Oreochromis niloticus) and Common carp (Cyprinus carpio) mortality were occurred in August and September, 2016. Data were collected from four stations on Maninjau lake (Muko-Muko, Pasa, Pandan and Sungai Tampang). Water samples were taken from the surface (depth 0.1 m) and under floating net cages (depth 30 m) at each station and analyzed for N, P and TOM content. Water quality data taken in February, 2016 was used as a comparison. The differences between N, P, TOM, Particulate Organic Matter (POM) and Dissolved Organic Matter (DOM) levels before and after fish mass mortality were analyzed using a student t-test. Any differences between stations were analyzed using one-way ANOVA was performed using SPSS computer software. Results: The levels of N and P before and after fish mass mortality were significantly different (p<0.05). The N levels at surface ranged from 1.83-2.30 mg L–1. At 30 m, N levels ranged from 2.11-2.60 mg L–1. The P levels ranged from 0.50-0.91 mg L–1 and 0.81-0.92 mg L–1 at 0.1 and 30 m depths, respectively. The N level tended to the limiting factor for algae growth at each station (all N/P<16). The TOM levels before and after fish mass mortality ranged from (Mean±SD) 4.55±0.02-16.33±0.01 mg L–1 and 6.97±0.72-19.04±0.04 mg L–1 at 0.10 and 30 m depths, respectively. Conclusion: The availability of N, P and TOM in the water was significantly higher (p<0.05) after fish mass mortality and had a negative effect on the water quality of Maninjau lake.
 
 
 
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