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Articles
by
Jun-Heon Lee |
Total Records (
3 ) for
Jun-Heon Lee |
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Hyun-Kyung So
,
P.K. Mandal
,
O. Baatartsogt
,
Hee-Kyong Lim
,
Chi-Ho Lee
,
Jun-Heon Lee
and
Kangduk Choi
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To find the alternative for antibiotic this study was carried out to investigate the differentially expressed proteome between Salmonella gallinarum infected and uninfected control in the spleen lymphocytes of ROS broiler chicks fed with Korean mistletoe using proteomic approach. Total four protein spots were detected with differential expression from the chicken spleen lymphocyte in 2DE gels after silver staining. These proteins were characterized by MALDI-TOF MS and MS/MS. Two known proteins were up-regulated viz., Fatty Acid Binding Protein (FABP) and MRP-126 and 2 proteins were down regulated viz., ribosomal protein12, pyruvate kinase. In this experimental fowl typhoid infection in broilers fed with Korean mistletoe through proteomics approach significant differential expression of four proteins were found which appears to be candidate molecules for fowl typhoid. |
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Oyungerel Baatartsogt
,
Prabhat Kumar Mandal
,
Hee-kyong Lim
,
Chi-Ho Lee
,
Jun-heon Lee
and
Kangduk Choi
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To understand the pathogenesis of PTA we studied the gene expression profiles of synovial cells of knee using gene chip analysis. PTA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in 3 piglets. After sacrificing the piglet at 8 weeks, synovial cells were used for gene expression profiling using Affimetrix GeneChip. Affimetrix GeneChip Operating Software and Robust Multi-array Analysis (RMA) were used to identify differentially expressed genes. Expressions of selected genes were verified by RT-PCR. Histopathologic examination at 8 weeks showed overt chronic inflammation indicating the development of PTA. Through genome analyses it was observed that 87 known genes were up-regulated and 76 known genes were down regulated. By analyzing gene expression, we found differential expression of genes related to inflammatory and immune response, lipid binding, cell adhesion, growth activity and muscle development. Some of the important genes related to inflammatory and immune response are FAS, MCP-1, IL-18, IL-1α, IL-6, IL-7, OAS1, TLR4, MX2. RT-PCR result has confirmed the expression of selected genes (IL 6, IL 18, FABP 4, LPL and PLTP) in total RNA isolated from synovial cells. The genome analysis of synovial cells provided us new candidate molecules related to inflammation and immunity which may be useful to understand the pathogenesis of PTA. |
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O. Baatartsogt
,
K. Choi
,
P.K. Mandal
,
Hee-kyong Lim
,
Guan-Hao Li
,
Hong-Sik Kong
,
Dae-Hyun Hahm
,
Chi-Ho Lee
and
Jun-Heon Lee
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To get insights into pathological pathways of Post Traumatic
Arthritis (PTA) this study was done on gene expression profile of synovial
cells of knee using gene chip analysis developing experimental post traumatic
arthritis (ETA) in swine. ETA was induced by transection of the Anterior
Cruciate Ligament (ACL) of left knee in 3 piglets. Articular cartilage
and synovial tissues were obtained after 0, 2, 5 and 8 weeks for histopathologic
examination. Synovial cells collected after sacrificing the piglet at
8 weeks, were used at 5 passage for gene expression profiling using Affimetrix
Gene Chip. Histopathologic examination showed overt chronic inflammation
indicating the development of ETA. Through genome analyses it was observed
that 87 known genes were up-regulated and 76 known genes were down regulated.
By analyzing, it was found that many genes with differential expression
are related to inflammation, immune response, lipid binding, cell adhesion,
growth activity and muscle development. The present study provided an
insight into the TA related gene expression pattern. The genome analysis
of synovial cells provided us new candidate molecules which may be useful
to understand the pathogenesis of Post Traumatic Arthritis (PTA). The
established porcine model may serve as in vivo disease model for
further research on traumatic arthritis to elucidate molecular pathogenesis. |
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