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Articles by Jun Yang
Total Records ( 7 ) for Jun Yang
  Gongping Sun , Xuefeng Xu , Yingshuo Wang , Xiaoyun Shen , Zhimin Chen and Jun Yang
  Mycoplasma pneumoniae is a frequent cause of community-acquired bacterial respiratory infections in children and adults. In the present study, using a proteomic approach, we studied the effects of M. pneumoniae infection on the protein expression profile of A549 human lung carcinoma cells. M. pneumoniae infection induced changes in the expression of cellular proteins, in particular a group of proteins involved in the oxidative stress response, such as glucose-6-phosphate 1-dehydrogenase, NADH dehydrogenase (ubiquinone) Fe-S protein 2, and ubiquinol-cytochrome c reductase complex core protein I mitochondrial precursor. The oxidative status of M. pneumoniae-infected cells was evaluated, and the results revealed that M. pneumoniae infection indeed caused generation of reactive oxygen species (ROS). It was further shown that M. pneumoniae infection also induced DNA double-strand breaks, as demonstrated by the formation of γH2AX foci. On the other hand, an ROS scavenger, N-acetylcysteine, could inhibit the ROS generation, as well as decrease γH2AX focus formation. This is the first report showing that M. pneumoniae infection can directly induce DNA damage, at least partially, through the generation of ROS, and thus this report strengthens the powerful application of proteomics in the study of the pathogenesis of M. pneumoniae.
  Fahim Ullah , Mansoor Khan Khattak , Min Kang , Ninghui Li , Jun Yang and Xingsheng Wang
  Background and Objectives: In this study, the numerical simulation of the steady-state thermal performance of flat plate solar collector with double glass cover, physical model, validation and its grid independence test were performed with the finite volume method. Moreover, through comparison with the conventional plate heat collector, the effects of ambient temperature, the inlet water temperature, solar radiated intensity and lower glass cover position on thermal performance of the heat collector were investigated. Materials and Methods: For the simulation of FPSC with double glass covers different physical model, geometric and boundary condition were used and also regression analysis was applied on the data. Results: From the investigated results, it was cleared that, when the ambient temperature is below 18°C, the collector with double glass covers shows advantages, while when the ambient temperature is -10°C, the instantaneous efficiency of the flat plate solar collector with double glass covers was noted 0.26 higher than that of the conventional plate heat collector. Similarly, increase in the inlet water temperature and solar radiate intensity both results in an increase in the temperature inside the collector, which enhances the insulation effect of the double glass covers. Conclusion: When the inlet water temperature was 50°C, the instantaneous efficiency difference between the flat-plate solar collector with the double glass covers and the conventional one increases to 0.27. Furthermore, concluded from the results of the experiment that, when the lower glass cover is located 18 mm over the absorber plate and the solar radiated intensity is to be 1000 W m–2, the instantaneous efficiency of the flat-plate solar collector with the double glass covers can reach up to 0.678.
  Jun Chen , Jihong Liu , Tao Wang , Hengjun Xiao , Chunping Yin , Jun Yang , Xiaowen Chen and Zhangqun Ye
  The relaxation mechanisms of tetrandrine (Tet) on the rabbit corpus cavernosum tissue in vitro were investigated. Strips of rabbit corpus cavernosum were mounted in organ chambers. The effects of Tet were examined on isolated muscle strips pre-contracted with phenylephrine (PE) alone, in the presence of NW-nitro-L-arginine (LNNA, a nitric oxide synthase inhibitor), 1-H-[1,2,4]oxadiazolo[4,3-α]quinoxalin-1-one(ODQ, a guanylyl cyclase inhibitor), indomethacin (cyclooxygenase inhibitor), tetraethylammonium (TEA, Ca2+-activated K+ channel blocker), 4-aminopiridine (4-AP, voltage dependent K+ channel blocker) and glibenclamide (ATP sensitive K+channel blocker). The effects of Tet on KCl-induced contraction of isolated muscle strips were also investigated. The procedure of calcium absence-calcium addition was designed to observe the effect of Tet on the two components of the contractile responses to PE based on the source of Ca2+ (extracellular vs. intracellular). Corpus cavernosum strips showed relaxation in response to Tet (10-8 ~ 10-3 mol L-1) in a concentration-dependent manner with an IC50 of 3.73 x 10-5 mol L-1. However, they were not affected by LNNA, ODQ, indomethacin and K+-channel blockers. Tet (10 μmol L-1, 30 μmol L-1) concentration dependently reduced the maximal contraction response of isolated strips induced by KCl to (73.0 ± 3.8) and (41.5 ± 3.4)%, respectively (p < 0.01). In the procedure of calcium absence-calcium addition, Tet 100 μmol L-1 inhibited both intracellular calcium-dependent and extracellular calcium-dependent contraction induced by PE (20 μmol L-1) (p < 0.05). The inhibition ratios were (23.8 ± 7.1) and (40.7 ± 11.2)%, respectively. The results of the present study suggest that Tet possesses a relaxant effect on rabbit corpus cavernosum tissues, which is attributable to the inhibition of extracellular Ca2+ influx and the inhibition of release of intracellular-stored Ca2+, but not mediated by the release of nitric oxide, prostaglandins or by the activation of potassium channels.
  Jun Yang , Yu Yang , Cheng-Hai Wang , Gen Wang , Hongtao Xu , Wen-Yan Liu and Bao-Cheng Lin
  Arginine vasopressin (AVP) has been proven to be involved in the process of pain regulation. This communication was designed to investigate the effect of AVP on acupuncture analgesia in the rat model. The results showed that intraventricular injection (icv) of AVP could enhance acupuncture analgesia in a dose-dependent manner, whereas icv of anti-AVP serum decreased acupuncture analgesia. However, neither intrathecal (ith) nor intravenous injection (iv) of AVP or anti-AVP serum could influence acupuncture analgesia. Electrical acupuncture of “Zusanli” points (St. 36) decreased AVP concentration in the hypothalamic paraventricular nucleus (PVN), and increased AVP concentration in the hypothalamic supraoptic nucleus (SON), periaqueductial gray (PAG), caudate nucleus (CdN) and raphe magnus nucleus (RMN), but did not change AVP concentration in the pituitary, spinal cord and plasma. The effect of AVP on acupuncture analgesia was partly reversed by pretreatment with naloxone, an opiate receptor antagonist. These data suggested that AVP in the brain played a role in the process of acupuncture analgesia in combination with the endogenous opiate peptide system.
  Jun Yang , Huifeng Yuan , Jiegen Chu , Yu Yang , Hongtao Xu , Gen Wang , Wen-Yan Liu and Bao-Cheng Lin
 

Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuropeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), β-endorphin (β-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA1–13 (DynA1–13), oxytocin, achetylcholine, choline, γ-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, β-Ep, DynA1–13, 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous opiate peptide and 5-HT system.

  Arkadiusz G. Klopocki , Tadayuki Yago , Padmaja Mehta , Jun Yang , Tao Wu , Anne Leppanen , Nicolai V. Bovin , Richard D. Cummings , Cheng Zhu and Rodger P. McEver
  Selectin-ligand interactions (bonds) mediate leukocyte rolling on vascular surfaces. The molecular basis for differential ligand recognition by selectins is poorly understood. Here, we show that substituting one residue (A108H) in the lectin domain of L-selectin increased its force-free affinity for a glycosulfopeptide binding site (2-GSP-6) on P-selectin glycoprotein ligand-1 (PSGL-1) but not for a sulfated-glycan binding site (6-sulfo-sialyl Lewis x) on peripheral node addressin. The increased affinity of L-selectinA108H for 2-GSP-6 was due to a faster on-rate and to a slower off-rate that increased bond lifetimes in the absence of force. Rather than first prolonging (catching) and then shortening (slipping) bond lifetimes, increasing force monotonically shortened lifetimes of L-selectinA108H bonds with 2-GSP-6. When compared with microspheres bearing L-selectin, L-selectinA108H microspheres rolled more slowly and regularly on 2-GSP-6 at low flow rates. A reciprocal substitution in P-selectin (H108A) caused faster microsphere rolling on 2-GSP-6. These results distinguish molecular mechanisms for L-selectin to bind to PSGL-1 and peripheral node addressin and explain in part the shorter lifetimes of PSGL-1 bonds with L-selectin than P-selectin.
  Willis K. Samson , Jian V. Zhang , Orna Avsian-Kretchmer , Kai Cui , Gina L. C. Yosten , Cindy Klein , Rong-Ming Lyu , Yong Xiong Wang , Xiang Qun Chen , Jun Yang , Christopher J. Price , Ted D. Hoyda , Alastair V. Ferguson , Xiao-bin Yuan , Jaw Kang Chang and Aaron J. W. Hsueh
  Somatostatin is important in the regulation of diverse neuroendocrine functions. Based on bioinformatic analyses of evolutionarily conserved sequences, we predicted another peptide hormone in pro-somatostatin and named it neuronostatin. Immuno-affinity purification allowed the sequencing of an amidated neuronostatin peptide of 13 residues from porcine tissues. In vivo treatment with neuronostatin induced c-Fos expression in gastrointestinal tissues, anterior pituitary, cerebellum, and hippocampus. In vitro treatment with neuronostatin promoted the migration of cerebellar granule cells and elicited direct depolarizing actions on paraventricular neurons in hypothalamic slices. In a gastric tumor cell line, neuronostatin induced c-Fos expression, stimulated SRE reporter activity, and promoted cell proliferation. Furthermore, intracerebroventricular treatment with neuronostatin increased blood pressure but suppressed food intake and water drinking. Our findings demonstrate diverse neuronal, neuroendocrine, and cardiovascular actions of a somatostatin gene-encoded hormone and provide the basis to investigate the physiological roles of this endogenously produced brain/gut peptide.
 
 
 
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