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Articles by Jun Wang
Total Records ( 14 ) for Jun Wang
  Jun Wang , Rui Wang and Xiao-Ye Yang
  For oral application biocontrol of animal parasitic nematode in ruminants, ion beam implantation nematode-trapping fungi must has the capacity to survive the passage through the digestive tract and be efficient in reducing infective larvae of nematodes in the faeces. Ion beam induced mutation the spores of Arthrobotrys oligospora of nematode-trapping. The mutant of the genetic stability is breeded. The fungi were cultured in bottles with corn kernels as a growth media and spores of different doses was respectively administered orally to each group of sheep naturally infected with gastrointestinal nematodes. The control group did not receive fungi. The faeces of these experimental animals were colleted and fecal cultivations carried out. Tested the fungal germination, growth, reproduction and predation livestock parasitic nematode larvae in laboratory. Test the efficacy of a Arthrobotrys oligospora N mutant in nematode-trapping larvae after passage through the digestive tract of sheep. In this research, these capacities were evaluated. The results indicated that ion beam implantation nematode-trapping fungi is a positive mutation. Mutant spores through the digestive tract of sheep can kill livestock parasite nematode larvae in vitro. These results indicate the potential of Arthrobotrys oligospora N mutant as a biological control agent for sheep nematodes. This study showed that such biotechnology can be explored for improving the effectiveness of the use of fungal infections to control livestock parasitic nematodes. This research represents the first application of nematode-trapping fungi in eukaryotic microorganisms.
  Guo-qiang Li , Shan-shan Li , Ming-lu Zhang , Jun Wang , Lin Zhu , Feng-lai Liang , Ru-lin Liu and Ting Ma
  Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and translation of dszA, dszB, and dszC decreased according to the positions of the genes in the dsz operon. Furthermore, the translation of dszB was repressed by an overlapping structure in the dsz operon. In order to get better and steady expression of the Dsz enzymes and optimize the metabolic flux of DBT, we rearranged the dsz operon according to the catalytic capabilities of the Dsz enzymes and expressed the rearranged dsz operon, dszBCA, in Rhodococcus erythropolis. After rearrangement, the ratio of dszA, dszB, and dszC mRNAs in the cells was changed, from 11:3.3:1 to 1:16:5. Western blot analysis revealed that the levels of expression of dszB and dszC had been enhanced but that the expression of dszA had decreased. The desulfurization activity of resting cells prepared from R. erythropolis DRB, which carried the rearranged dsz operon, was about 12-fold higher than that of resting cells of R. erythropolis DRA, which carried the original operon in a similarly constructed vector.
  Zhengping Mao , Jing Ma , Jun Wang and Baode Sun
  TiN-based composite coatings with and without the addition of Cr were deposited by reactive plasma spraying (RPS) in air. Both sintered and mixed powder of Ti and B4C were used for the RPS process. A thermodynamic model was firstly used to estimate the complicated phase composition of composite coatings prepared by RPS. The phase composition, structures and properties of TiN-based coatings were investigated using XRD, SEM and a Vickers microhardness tester. The results show that the phases in TiN-based coatings do not generate according to priority of Gibbs free energy value due to non-equilibrium reactive course during thermal spraying. The coating deposited using sintered Ti and B4C powder is composed of two main phases (TiN and TiN0.3), two minor phases (Ti2O3 and TiB2), and a small fraction of TiC phase. The composition of the coating deposited using the mixed powder with Cr added is predominantly in the TiN and TiB2 phases, a smaller phase fraction of Ti2O3 and TiO2, and some unreacted Cr. The Vickers microhardness of the coating deposited using sintered powder is higher than that of using mixed powder. The composite coating deposited using mixed powder with the addition of Cr shows superior corrosion resistant to that using sintered powder when tested in 3.5wt.% NaCl electrolytic solution.
  Kai-Yu Wang , Hai Lian , De-Fang Chen , Jin-Lu Huang , Jun Wang and Qiao-Feng Mou
  The p1 gene of Yersinia ruckeri (yrp1) which was isolated from channel catfish was amplified by PCR with specific primers and inserted into pMD19-T vector. The positive recombinant plasmid was selected and sequenced. Molecular characteristics analysis of the yrp1 gene and the protein which is encoded by it was performed. The results showed that the yrp1 gene is 1434 bp in length with G+C content of 44.35%. The analysis of codon bias indicated that the codon usage frequency of the yrp1 gene is distinctly different and it is preferable to perform in E. coli and yeast. The theoretical relative molecular mass and iso-electric point of the Yrp1 amino acid sequence are about 51.5 kDa and 4.48 separately. The polypeptide has some important sites related to post-translational modification, including 35 potential phosphorylation sites and 4 potential N-glycosylation sites. The polypeptide analyzed in this study contains a ZnMc superfamily conserved domains and does not contain a signal peptide, even though it is a secretory protein.
  Mamadou Samake , Zhonghua Tang , Win Hlaing and Jun Wang
  Bamako capital of Mali is the most densely populated and urbanized city of this developing country. Its annual growth rate of the population during the last decade was 3.7%. Bamako is also an administrative and commercial centre, providing many facilities that contribute to attract many people in the city. Thus, the amount of wastes, mainly municipal solid waste, generated is increasing rapidly in parallel with the increasing size of population. Bamako produced about 325,807 m3 in 2007 and this amount is supposed to increase crescendo each year. Municipal solid waste management, notwithstanding the great part of local government budget remains a sector with a large gap. The MSW is disposed in uncontrolled open discharge at the outskirt of the city or even stays in the city in the form of anarchic or illegal rubbish dumps. Moreover, there is no appropriate infrastructure for household wastes treatment. This study points out and evaluates the state of MSW management in Bamako. We summarize the policy and legislative framework; establish a link between rapid population growth and the increasing of MSW generation. Finally, the study gives some details about MSW collection system in Bamako.
  Ting Long , Zhi-Jun Yu , Jun Wang , Jia Liu and Bing-Shu He
  Background and Objective: The alterations in the gut microbiota composition are gaining increasing attention in view of their influence on the development of ulcerative colitis. The anti colitis effect of orally administered chitooligosaccharides (COS) had been reported in the animal models of ulcerative colitis but the mechanism is still uncertain. Interestingly, COS have long been proposed as potential natural prebiotics based on in vitro experiments. The aim of this study is to confirm the prebiotic property of COS in vivo and further clarify the mechanisms of their anti colitis effect. Materials and Methods: The COS at the dose of 500 mg kg–1 were orally given normal mice and colitis mice treated by 3.5% dextran sulfate sodium (DSS). The colon microbial composition in mice was evaluated by qualitative analysis of 16S ribosomal DNA in colonic content samples using real-time PCR. Results: The COS could function as prebiotics by increasing the levels of Bacteroidetes and Actinobacteria phyla, the relative ratio of Bacteroidetes to Firmicutes, as well as common probiotics such as Lactobacillus and Bifidobacterium and inhibiting the growth of Firmicutes and Proteobacteria phyla, as well as potential pathogens such as Enterococcus, in both normal and colitis mice. In addition, oral intake of COS were found to enhance the colonic concentrations of short-chain fatty acids (SCFAs), the dominating fermentation end-products of bacteria in the large bowel having abilities to support the transport processes, energy metabolism, cellular growth and differentiation of colonocytes. Conclusion: The data suggested that COS administration might had beneficial effects on the health of the intestinal tract and more importantly, tended to protect mice from dysbiosis of native gut microbiome and against the suppression of SCFA production, which might be a potential mechanism for their anti colitis effect.
  Hai Lian , Kai-Yu Wang , De-Fang Chen , Jun Wang , Ling-Yuan Huang and Cheng-Wei Li
  The p1 gene of Yersinia ruckeri (yrp1) which was isolated from channel catfish was amplified by PCR with specific primers and inserted into pMD19-T vector. The positive recombinant plasmid was selected and sequenced. Then, the yrp1 gene was subcloned into pET-32a (+) vector and transformed into BL21 (DE3) followed by induction with IPTG and detection with SDS-PAGE. Optimization of the induction conditions were conducted. The results showed that the recombinant protein with a molecular mass of about 72 kDa was mostly packaged into inclusion bodies. The optimization of induction process conditions led us to perform the fusion protein induction at 37°C for 4 h with 0.8 mM IPTG.
  Jun Wang , Dan Li , Jingqun Gao , Bin Liu , Baoxin Wang , Dan Wang , Tingting Fan and Xiangdong Zhang
  Two complexes, (enH2)3[TbIII(ttha)]2 · 11H2O (1) (en = ethylenediamine and H6ttha = triethylenetetramine-N, N, N ', N '', N ''', N '''-hexaacetic acid) and (enH2)[TbIII(pdta)(H2O)]2 · 8H2O (2) (H4pdta = propylenediamine-N, N, N ', N '-tetraacetic acid), were synthesized and characterized by elemental analysis, infrared spectrum, UV-Vis spectrum, fluorescence spectrum, and single-crystal X-ray diffraction. The central TbIII of 1 is nine-coordinate, pseudo-monocapped square antiprism with four nitrogens and five oxygens from one ttha, and crystallizing in the monoclinic crystal system with P21/n space group. There is a free (non-coordinate) carboxylate (-CH2COO-) in the [TbIII(ttha)]3-. The central TbIII of 2 is eight-coordinate in a standard square antiprism with two nitrogens and four oxygens of one pdta, one oxygen from a carboxylate of an adjacent pdta, and one oxygen from water, crystallizing in the monoclinic crystal system with C2/c space group. Binding between the enH22+ with [TbIII(ttha)]3- or [TbIII(pdta)(H2O)]- is reviewed, providing the basis for interaction of TbIII complexes with biomolecules.
  Jingqun Gao , Dan Li , Jun Wang , Xudong Jin , Pingli Kang , Tong Wu , Kai Li and Xiangdong Zhang
  A 2-D binuclear coordination polymer, {[YIII(Hpdta)(H2O)]2 ·6H2O} n (H4pdta = propylenediamine-N,N,N′,N′-tetraacetic acid), has been synthesized through direct hydrothermal reaction and characterized by infrared spectrum and thermal analysis. Single-crystal X-ray diffraction reveals that Y(III) is eight-coordinate of almost standard square antiprismatic polyhedron.
  Jingqun Gao , Dan Li , Jun Wang , Xudong Jin , Tong Wu , Kai Li , Pingli Kang and Xiangdong zhang
  Two lanthanide complexes, (enH2)[SmIII(egta)(H2O)]2 • 6H2O (1) (en = ethylenediamine and H4egta = ethyleneglycol−bis−(2−aminoethylether)−N,N,N′,N′−tetraacetic acid) and (enH2)1.5[SmIII(ttha)] • 4.5H2O (2) (H6ttha = triethylenetetramine−N,N,N′,N″,N′″,N′″−hexaacetic acid), have been synthesized and characterized by IR spectroscopy, thermal analysis, and single-crystal X−ray diffraction. The (enH2)[SmIII(egta)(H2O)]2 • 6H2O is mononuclear nine−coordinate and crystallizes in the monoclinic crystal space group P21/n with the cell dimensions a = 13.0563(13) Å, b = 12.6895(11) Å, c = 14.9497(15) Å, β = 105.782(2)°. The polymeric (enH2)1.5[SmIII(ttha)] • 4.5H2O is also nine−coordinate crystallizing in the monoclinic crystal space group P21/n, but with cell dimensions a = 17.7800(16) Å, b = 9.7035(10) Å, c = 22.096(2) Å, β = 118.8740(10)°. Each ethylenediammonium (en ) cation in (enH2)[SmIII(egta)(H2O)]2 • 6H2O connects three adjacent [SmIII(egta)(H2O)] anions through hydrogen bonds, while in (enH2)1.5[SmIII(ttha)] • 4.5H2O, there are two types of en cations, which form hydrogen bonds with the neighboring [SmIII(ttha)]3− anions, leading to the formation of a 2−D ladder-like layer structure.
  Nathan Topham , Jun Wang , Mark Kalivoda , Joyce Huang , Kuei-Min Yu , Yu-Mei Hsu , Chang-Yu Wu , Sewon Oh , Kuk Cho and Kathleen Paulson
  Hexavalent chromium (Cr6+) emitted from welding poses serious health risks to workers exposed to welding fumes. In this study, tetramethylsilane (TMS) was added to shielding gas to control hazardous air pollutants produced during stainless steel welding. The silica precursor acted as an oxidation inhibitor when it decomposed in the high-temperature welding arc, limiting Cr6+ formation. Additionally, a film of amorphous SiO2 was deposited on fume particles to insulate them from oxidation. Experiments were conducted following the American Welding Society (AWS) method for fume generation and sampling in an AWS fume hood. The results showed that total shielding gas flow rate impacted the effectiveness of the TMS process. Increasing shielding gas flow rate led to increased reductions in Cr6+ concentration when TMS was used. When 4.2% of a 30-lpm shielding gas flow was used as TMS carrier gas, Cr6+ concentration in gas metal arc welding (GMAW) fumes was reduced to below the 2006 Occupational Safety and Health Administration standard (5 μg m-3) and the efficiency was >90%. The process also increased fume particle size from a mode size of 20 nm under baseline conditions to 180-300 nm when TMS was added in all shielding gas flow rates tested. SiO2 particles formed in the process scavenged nanosized fume particles through intercoagulation. Transmission electron microscopy imagery provided visual evidence of an amorphous film of SiO2 on some fume particles along with the presence of amorphous SiO2 agglomerates. These results demonstrate the ability of vapor phase silica precursors to increase welding fume particle size and minimize chromium oxidation, thereby preventing the formation of hexavalent chromium.
  Rachel Jurd , Claire Thornton , Jun Wang , Ken Luong , Khanhky Phamluong , Viktor Kharazia , Stuart L. Gibb and Dorit Ron
  The N-methyl-D-aspartate receptor (NMDAR) plays a critical role in synaptic plasticity. Post-translational modifications of NMDARs, such as phosphorylation, alter both the activity and trafficking properties of NMDARs. Ubiquitination is increasingly being recognized as another post-translational modification that can alter synaptic protein composition and function. We identified Mind bomb-2 as an E3 ubiquitin ligase that interacts with and ubiquitinates the NR2B subunit of the NMDAR in mammalian cells. The protein-protein interaction and the ubiquitination of the NR2B subunit were found to be enhanced in a Fyn phosphorylation-dependent manner. Immunocytochemical studies reveal that Mind bomb-2 is localized to postsynaptic sites and colocalizes with the NMDAR in apical dendrites of hippocampal neurons. Furthermore, we show that NMDAR activity is down-regulated by Mind bomb-2. These results identify a specific E3 ubiquitin ligase as a novel interactant with the NR2B subunit and suggest a possible mechanism for the regulation of NMDAR function involving both phosphorylation and ubiquitination.
  Jun Wang , Hua Zhang , Dinakar Iyer , Xin-Hua Feng and Robert J. Schwartz
  The cardiac specific homeobox gene nkx2.5, a member of the nk-2 class family, plays a central role in cardiogenesis and is a target of the small ubiquitin-like modifier (SUMO). Nkx2.5 was modified by SUMO on its 51st amino acid, a lysine residue conserved across species but absent in other nk-2 members. Conversion of this lysine to an arginine (K51R) substantially reduced Nkx2.5 DNA binding and also its transcriptional activity. Unexpectedly, mutant K51R was targeted by ubiquitin. E3 ligase PIAS proteins PIAS1, PIASx, and PIASy, but not PIAS3, enhanced SUMO-1 attachment to Nkx2.5 on the primary SUMO acceptor site. SUMO-2 linkage to Nkx2.5 was catalyzed only by PIASx and not by other PIAS proteins. SUMO conjugation stabilized the formation of Nkx2.5-containing complexes that led to robust transcriptional activation. Thus, SUMO modification serves as a positive regulator for Nkx2.5 transcriptional activity.
  Kenjiro Ono , Margaret M. Condron , Lap Ho , Jun Wang , Wei Zhao , Giulio M. Pasinetti and David B. Teplow
  Epidemiological evidence suggests that moderate consumption of red wine reduces the incidence of Alzheimer disease (AD). To study the protective effects of red wine, experiments recently were executed in the Tg2576 mouse model of AD. These studies showed that a commercially available grape seed polyphenolic extract, MegaNatural-AZ (MN), significantly attenuated AD-type cognitive deterioration and reduced cerebral amyloid deposition (Wang, J., Ho, L., Zhao, W., Ono, K., Rosensweig, C., Chen, L., Humala, N., Teplow, D. B., and Pasinetti, G. M. (2008) J. Neurosci. 28, 6388–6392). To elucidate the mechanistic bases for these observations, here we used CD spectroscopy, photo-induced cross-linking of unmodified proteins, thioflavin T fluorescence, size exclusion chromatography, and electron microscopy to examine the effects of MN on the assembly of the two predominant disease-related amyloid β-protein alloforms, Aβ40 and Aβ42. We also examined the effects of MN on Aβ-induced cytotoxicity by assaying 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide metabolism and lactate dehydrogenase activity in Aβ-treated, differentiated pheochromocytoma (PC12) cells. Initial studies revealed that MN blocked Aβ fibril formation. Subsequent evaluation of the assembly stage specificity of the effect showed that MN was able to inhibit protofibril formation, pre-protofibrillar oligomerization, and initial coil → α-helix/β-sheet secondary structure transitions. Importantly, MN had protective effects in assays of cytotoxicity in which MN was mixed with Aβ prior to peptide assembly or following assembly and just prior to peptide addition to cells. These data suggest that MN is worthy of consideration as a therapeutic agent for AD.
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