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Articles by Jun Fang
Total Records ( 4 ) for Jun Fang
  Jun Fang , Meihu Ma , Yongguo Jin , Ning Qiu , Chan Wang , Guodong Ren and Xin Huang
  Egg white was considered to play an important role in bacterial-resistance nature of developing embryo especially during early incubation. Previous works were not quite informative in the antimicrobial action of egg white. This study was performed to investigate the survival ability of Salmonella enteritidis in egg white during early incubation and to tentatively reveal underline anti-Salmonella enteritidis mechanisms of egg white using the combination of flow cytometric analysis and fluorescent strategy. Such properties were also tested at different incubation temperature and pH. The traditional plate count method was also included in this study and the results were in accordance with those of flow cytometric assessment. Following egg white treatment, Salmonella enteritidis was discriminated into three subpopulations by flow cytometric assessment: viable, dead and membrane-compromised cells. Furthermore, the results showed that with the extension of incubation time, the population giving green fluorescence increased steadily while red fluorescence emittance didn’t change a lot. The incubation temperature and pH were both significant factors in anti-Salmonella enteritidis properties of egg white. Besides, the main target for antimicrobial factors in egg white on Salmonella enteritidis cells was membrane integrity and the action mechanism of egg white was bacteriostatic rather than bactericidal effect during early incubation.
  Xiaohong Yuan , Pisheng Qu , Jun Fang , Shunv Cai and Shuang Fu
  Background and Objective: Epidural anesthesia is reduced opioid and anesthetic consumption, facilitates pain control and ameliorates surgical stress. Propofol and sevoflurane have also made changes in immune responses. The objective of the study was to evaluate the effect of pre-operational epidural sevoflurane and propofol anesthesia on cellular immunity of patients following lung cancer surgery. Materials and Methods: Total 246 patients with lung cancer who had planned for thoracotomy subjected to randomize (1:1 ratio) and divided into two groups of 123 patients each. Anesthesia was maintained by sevoflurane at 2 μg mL–1 alveolar concentration (group I) or a continuous infusion of propofol at 2-4 μg mL–1 steady-state plasma concentrations (group II). The killing activity of natural killer T-cells, western blot, T-helper cells assay, phagocytosis assay and cell proliferation assay had been evaluated. Wilcoxon test/Tukey’s post hoc tests were performed between pre-operational and post-operative data at 95% of confidence interval. Results: Sevoflurane (p = 0.6217) had no effect and propofol (p<0.0001, q = 10.835) had depressed CD 4+/CD 8+ ratio. Sevoflurane (p<0.0001, q = 40.473) had decreased but propofol (p = 0.058) had no effect on interferon-γ; Interleukin-4 ratio. Sevoflurane had decreased and propofol had boosted the counts of T-cells, T-helper cells and killing activity natural killer T-cells. Unlike, sevoflurane, propofol had increased interleukin-5 counts. Propofol and sevoflurane both had a significant cell proliferation and phagocytosis effects. Conclusion: Type of anesthetic used modulates the immunity of patients undergoing lung cancer resection.
  Wang Shengping , Jun Fang , Tang Xiangshan , Wang Jie , Zhichun Zhan , Ying Zhou , Xiaosong Wu , Dingding Su , Gang Liu , Jiannan Ding and Lichuang Cai
  NSP is the major factor introducing a low digestibility of cottonseed meal in piglets. The present study was conducted to determine the effect of NSP enzyme on the nutrient digestibility of weaned piglets fed a cottonseed meal-based diets. About 15 weaned piglets (14±1.4 kg) were selected and ileal fistula surgery was done to determine the DE, CP, DM and NSP’s apparent digestibility. The results indicated that NSP enzyme improved piglet’s apparent digestibility (p<0.05) in varying degrees; pectinase resulted in obvious degradation of the cell wall and increased piglets’ DE (26.69%) and CP of ileal digesta (20.33%) and compound enzyme was better in improving cottonseed meal based diets than single enzyme.
  Gang Liu , Wenkai Ren , Dingding Su , Miaomiao Wu , Yinghui Li , Wei Luo , Xinglong Yu , Tiejun Li and Jun Fang
  This study was conducted to test the hypotheses that dietary L-arginine supplementation may enhance the immune responses and resulting in the clearance against PCV2 in experimentally infected mice. The measured variables include: the PCV2 virus load in liver, spleen, heart, lung, kidney, ovary and serum on 3rd, 5th, 7th, 9th and 11th day post infection (dpi); serum Interleukin-2 (IL-2), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Interferon alpha (IFN-α), Interferon gamma (IFN-γ) and C-Reactive Protein (CRP) levels on 3rd, 5th, 7th, 9th and 11th dpi; serum Total Superoxide Dismutase (T-SOD) activity on 3rd, 5th, 7th, 9th and 11th dpi. Results showed that arginine supplementation could significantly increase the serum IL-2 levels on the 9th and 11th dpi; significantly increase the serum IFN-α and CRP levels on the 11th dpi; significantly increase the serum IFN-r levels on the 7th dpi and significantly decrease the serum IL-6 levels on the 9th dpi. Meanwhile, the PCV2 virus genome was detected sporadically. Collectively, dietary L-arginine supplementation had beneficial effects on the cytokines profile in the PCV2 infected mouse and maybe could delay the PCV2 replication and/or clear the PCV2 in mouse model.
 
 
 
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